Tamoxifen metabolite 4 hydroxytamoxifen 4 oht

Manufactured by Merck Group

Tamoxifen metabolite 4-hydroxytamoxifen (4-OHT) is a chemical compound used in laboratory research. It is a major active metabolite of the drug tamoxifen. 4-OHT is commonly used as a research tool to study the effects of tamoxifen and its mechanisms of action.

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Lab products found in correlation

5 aza 2 deoxycytidine 5 aza, by Merck Group (1 mentions) Estradiol e2, by Merck Group (1 mentions) Anti gapdh, by Santa Cruz Biotechnology (1 mentions) Anti h3k27me3, by Cell Signaling Technology (1 mentions) Anti cbp, by Abcam (1 mentions) Anti ncor, by Abcam (1 mentions) Anti ezh2, by Active Motif (1 mentions) Anti p300, by Fortis Life Sciences (1 mentions) Anti p300, by Santa Cruz Biotechnology (1 mentions) Anti h3, by Abcam (1 mentions) Anti erα, by Santa Cruz Biotechnology (1 mentions) Anti ha, by BioLegend (1 mentions) Anti ezh2, by BD (1 mentions) Abemaciclib mesylate, by Selleck Chemicals (1 mentions) Ab64883, by Abcam (1 mentions) Fulvestrant, by Selleck Chemicals (1 mentions) Anti β actin 60008 1 lg, by Proteintech (1 mentions) Normal igg peroxidase conjugated affinipure goat anti rabbit mouse igg h l, by Jackson ImmunoResearch (1 mentions) Palbociclib hcl, by Selleck Chemicals (1 mentions)

Close spelling variants of this product

Tamoxifen (3381 citations) 4-hydroxytamoxifen (577 citations) 4-OHT (320 citations) 4-hydroxytamoxifen (4-OHT) (282 citations) 4-hydroxytamoxifen (OHT, (20 citations) Hydroxytamoxifen (17 citations) 4-OHT Tamoxifen (15 citations)

2 protocols using tamoxifen metabolite 4 hydroxytamoxifen 4 oht

Antibody-based Protein Analysis in Cancer

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Antibodies being used in this study for Western blots include: anti-GREB1 (#ab72999; Abcam), anti-EZH2 (#612666; BD Biosciences), anti-ERα (#sc-543; Santa Cruz Biotechnology), anti-p300 (#A300-358A; Bethyl Laboratories), anti-CBP (#NB100-382; Novus Biologicals), anti-NCoR (#ab24552; Abcam), anti-HA (#901501; Biolegend), anti-H3K27me3 (#9733; Cell Signaling Technology), anti-GAPDH (#sc-365062; Santa Cruz Biotechnology), and anti-H3 (#ab1791; Abcam). Antibodies for ChIP assays include: anti-ERα (#sc-543; Santa Cruz Biotechnology and #MS-315; Thermo Scientific), anti-EZH2 (#39933; Active Motif), anti-H3K27me3 (#9733; Cell Signaling Technology), anti-p300 (#sc-48343X; Santa Cruz Biotechnology), anti-CBP (#ab2832; Abcam and #sc-7300; Santa Cruz Biotechnology), and anti-NCoR (#ab24552; Abcam). Antibodies used for IHC staining are mentioned below in the “IHC and quantification of IHC analysis” section. EZH2 inhibitors were purchased from Xcess Biosciences Inc. (GSK126, GSK343, and EPZ-6438). Estradiol (E2), tamoxifen metabolite 4-hydroxytamoxifen (4-OHT), and 5-Aza-2′-deoxycytidine (5-Aza) were obtained from Sigma, unless otherwise indicated.

Wu Y., Zhang Z., Cenciarini M.E., Proietti C.J., Amasino M., Hong T., Yang M., Liao Y., Chiang H.C., Kaklamani V.G., Jeselsohn R., Vadlamudi R.K., Huang T.H., Li R., De Angelis C., Fu X., Elizalde P.V., Schiff R., Brown M, & Xu K. (2017). Tamoxifen Resistance in Breast Cancer Is Regulated by the EZH2–ERα–GREB1 Transcriptional Axis. Cancer research, 78(3), 671-684.

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Western Blot Antibody Validation

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Antibodies used in immunoblotting: COL11A1 (ab64883, Abcam, 1:1000 dilution), ERα (No.8644, CST, 1:1000 dilution), anti-β-actin (60008-1-lg, Proteintech, 1:5000 dilution). Normal IgG/Peroxidase-conjugated AffiniPure Goat Anti-Rabbit/Mouse IgG (H + L) was purchased from Jackson Immuno Research. Tamoxifen metabolite 4-hydroxytamoxifen (4-OHT) was purchased from Sigma. Palbociclib HCl (#S1116), abemaciclib mesylate (#S7158), ribociclib HCl (#S5187) and fulvestrant (#S1191) were obtained from Selleck (Shanghai, China).

Fu C., Duan S., Zhou X., Meng Y, & Chen X. (2024). Overexpression of COL11A1 confers tamoxifen resistance in breast cancer. NPJ Breast Cancer, 10, 38.

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