VEGF, IGF-1, bFGF and SDF-1 were quantitatively evaluated by ELISA using the Quantikine system (R&D Systems Inc., Minneapolis, Minnesota). According to the manufacturer’s protocol, standards and pretreated samples were pipetted into the 96 wells. After washing away any unbound substances, an enzyme-linked polyclonal antibody specific for each growth factor was added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution was added to the wells and colour developed in proportion to the amount of growth factor bound in the initial step. The colour development was stopped and the intensity of the colour was measured using a microplate reader set to 450 nm. The obtained values were used to calculate the target protein concentration from a standard curve.
Quantikine system
Manufactured by R&D Systems
Sourced in United States
The Quantikine system is a quantitative sandwich immunoassay designed for the measurement of specific protein concentrations in biological samples. It utilizes pre-coated microplates and provides a convenient format for the analysis of multiple samples.
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Lab products found in correlation
2 protocols using quantikine system
1
Quantitative Growth Factor Analysis
2
Quantifying Inflammatory Cytokines in Mice
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Mouse adiponectin, IL-1β, IL-6, leptin, and TNFα were measured by a Quantikine system from R&D systems (MN, USA) according to the manufacturer's instructions. BMDM culture media was collected at the end of 24 h of LPS stimulation, and serum of mice fed the assigned experimental diet was collected by cardiac puncture. All samples and standards were measured in duplicate.
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