Initially, the entrance orifice of the canal and the cervical and middle thirds were prepared using Largo No. 2 (Dentsply Maillefer, Rio de Janeiro, RJ, Brazil) and No. 4, 3, and 2
Gates Glidden drills (Dentsply-Maillefer, Ballaigues, Switzerland), respectively, according to the crown-down technique. The working length was determined by inserting a size 15
Flexo-File (Dentsply-Maillefer) up to the apical foramen, and subtracting 1 mm from this measurement.
The K3 system (SybronEndo, Scafati, Italy) was used for instrumentation, with a file of size 55/0.04 as the final instrument. An amount of 2.5 mL of 2.5% NaOCl (Formula e Ação, São Paulo, SP, Brazil) was used at change of each file, using disposable syringes and 30 G
NaviTip needles (Ultradent, South Jordan, UT, USA) calibrated at 1 mm short of the working length. The root canals were dried with absorbent paper cones (Tanari, São Paulo, SP, Brazil), and filled using the lateral condensation technique and Fillapex sealer (Angelus, Londrina, PR, Brazil). Next, the specimens were stored at 37°C and 100% humidity for 7 days to allow the sealer to set.
Poletto D., Poletto A.C., Cavalaro A., Machado R., Cosme-Silva L., Garbelini C.C, & Hoeppner M.G. (2017). Smear layer removal by different chemical solutions used with or without ultrasonic activation after post preparation. Restorative Dentistry & Endodontics, 42(4), 324-331.