The concentrations of CX3CL1 (Cloud-Clone Corp., Katy, TX, USA), CX3CR1, CD200, and CD200R (all from Cusabio, Houston, TX, USA) in the frontal cortices and hippocampi of male rats at PND120 were measured using commercially available ELISA kits according to the manufacturer’s instructions. The detection limits were as follows: CX3CL1: 0.055 ng/mL, CX3CR1: 5.8 pg/mL, CD200: 11.75 pg/mL, and CD200R: 4.67 pg/mL, and the intra- and interassay precision values were CX3CL1: <10%, <12%, CX3CR1, CD200, and CD200R: <8%, <10%.
Cd200
Manufactured by Cusabio
Sourced in United States
The CD200 is a laboratory instrument designed for cell analysis. It is used to detect and quantify the expression of the CD200 surface protein on cells.
Automatically generated - may contain errors
Lab products found in correlation
Cx3cl1, by Cloud-Clone (3 mentions)
Cd200r, by Cusabio (3 mentions)
Cx3cr1, by Cusabio (3 mentions)
Interleukin 4 (il 4), by Cusabio (2 mentions)
Il 10, by Cusabio (1 mentions)
Interleukin 6 (il 6), by FineTest (1 mentions)
Tnf α, by Thermo Fisher Scientific (1 mentions)
Il 1β, by Cusabio (1 mentions)
Interleukin 6 (il 6), by Cusabio (1 mentions)
3 protocols using cd200
1
Quantifying Neuroinflammatory Markers in Rat Brains
2
Neuroimmune Markers in Rat Hippocampus and Cortex
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The protein levels of CX3CL1 (Cloud-Clone Corp., Katy, TX, USA); CX3CR1, CD200, CD200R, IL-1β, IL-4, IL-10, IBA1 (all from Cusabio, Houston, TX, USA); IL-6, TGF-β (Fine Test, Wuhan, Hubei, China); and TNF-α (Thermo Fisher Scientific, Waltham, MA, USA) in the hippocampi and frontal cortices (n = 6–8 in each group) of male rats at PND7 were measured using commercially available ELISA kits. The procedures were performed in accordance with the manufacturer’s instructions, and the minimum detectable doses were CX3CL1 0.055 ng/ml, CX3CR1 7.8 pg/ml, CD200 11.75 pg/ml, CD200R 4.67 pg/ml, IL-1β 15.6 pg/ml, TNF-α 16 pg/ml, IL-6 37.5 pg/ml, TGF-β 18.75 pg/ml, IL-4 3.9 pg/ml, IL-10 0.78 pg/ml and IBA1 6.25 pg/ml. Intra- and inter-assay precision values were CX3CL1 < 10%, <12%, and CX3CR1, CD200, CD200R, IL-1β, TNF-α, IL-6, TGF-β, IL-4, IL-10 and IBA1 < 8%, < 10%, respectively. The protein levels of CX3CL1, CX3CR1, CD200, CD200R and IBA1 were also assessed in the hippocampi and frontal cortices (n = 6–9 in each group) of adult male offspring (PND93).
3
Quantification of Neuroinflammatory Markers in Rat Brain
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The protein levels of CX3CL1 (Cloud-Clone Corp., Katy, TX, USA), CX3CR1, CD200, CD200R, IL-6, and IL-4 (all from Cusabio, Houston, TX, USA) in the frontal cortices and hippocampi of the male rats were measured using commercially available ELISA kits. The procedures were performed in accordance with the manufacturer’s instructions and the minimum detectable doses were: CX3CL1: 0.055 ng/mL, CX3CR1: 5.8 pg/mL, CD200: 11.75 pg/mL, CD200R: 4.67 pg/mL, IL-6: 0.78 pg/mL, IL-4: 3.9 pg/mL. Intra- and inter-assay precision values were: CX3CL1: <10%, <12%, CX3CR1, CD200, CD200R, IL-6, IL-4: <8%, and <10%, respectively.
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