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Human usp13 sirna

Manufactured by Merck Group
Sourced in United States

Human USP13 siRNA is a laboratory reagent designed for use in RNA interference (RNAi) research. It is a short, double-stranded RNA molecule that targets the USP13 gene, which encodes the deubiquitinating enzyme Ubiquitin Specific Peptidase 13. This product is intended for research use only and its specific applications should be determined by the user.

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2 protocols using human usp13 sirna

1

Transfecting TXNIP Plasmids and siRNA

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Human USP13 siRNA and control siRNA were purchased from Sigma Aldrich (St Louis, MO, USA). Cells were cultured on 60-mm dishes to 70–90% confluence. Plasmids encoding HA-tagged human TXNIP and TXNIP-S308A, empty vector, and siRNAs were transfected into IMR90 cells using PepMute siRNA transfection reagent or GenJet DNA transfection reagents (SignaGen, Rockville, MD) according to the transfection reagent manufacturer’s instructions.
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2

USP13 siRNA and Plasmid Transfection

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GenMute siRNA transfection reagent and LipoJet DNA transfection reagents were purchased from SignaGen (Rockville, MD, USA). Human Usp13 siRNA and control siRNA were purchased from Sigma Aldrich (St Louis, MO, USA). Cells were cultured on 6-well plates or 10-mm dishes to 70%−90% confluence. Plasmid encoding V5-tagged human USP13,22 (link) empty vector, and siRNAs were transfected into Mrc5 cells according to the transfection reagent manufacturer’s instructions.
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