Chromatographic conditions: HPLC ultraviolet detector (Shimadzu LC-2010AHT with LC Solution workstation); chromatographic column (Synergi™ 4 µm Hydro-RP 80 Å 250×4.6 mm, Phenomenex); mobile phase: acetonitrile: 0.1% formic acid aqueous solution = 14.5: 85.5 (v/v) (0–15 mins), acetonitrile: 0.1% formic acid aqueous solution = 40: 60 (v/v) (15.1–22 mins), acetonitrile: 0.1% formic acid aqueous solution = 14.5: 85.5 (v/v) (22.1–26.2 min); flow rate 1.0 mL/min; wavelength 310nm; injection volume 10µL.
Lc 2010aht
Manufactured by Shimadzu
Sourced in Japan, United States, France
The LC-2010AHT is a high-performance liquid chromatography (HPLC) system from Shimadzu. It is designed to perform automated liquid chromatography analysis with high precision and reliability. The system includes a high-pressure pump, an auto-sampler, a column oven, and a UV-Vis detector. It is capable of handling a wide range of analytical applications in various industries.
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Lab products found in correlation
Lc solution workstation, by Phenomenex (2 mentions)
Luna c8 column, by Phenomenex (2 mentions)
Uv vis dectector, by Shimadzu (2 mentions)
K alpha, by Thermo Fisher Scientific (2 mentions)
Synergi 4 m hydro rp 80 250 4.6 mm, by Phenomenex (1 mentions)
Gcms qp2010, by Shimadzu (1 mentions)
Spd 10a, by Shimadzu (1 mentions)
Eclipse xdb c18, by Agilent Technologies (1 mentions)
Zorbax, by Agilent Technologies (1 mentions)
Spd m10avp, by Shimadzu (1 mentions)
C18 guard column, by Phenomenex (1 mentions)
Visionht c18 column, by Shimadzu (1 mentions)
Hplc system, by Shimadzu (1 mentions)
Nano zs90, by Malvern Panalytical (1 mentions)
Fv1000, by Olympus (1 mentions)
Zetasizer nano zs90, by Malvern Panalytical (1 mentions)
Jem 1200ex, by JEOL (1 mentions)
Q tof hplc ms, by Agilent Technologies (1 mentions)
Isolera prime system, by Biotage (1 mentions)
Acme 9000, by Younglin (1 mentions)
54 protocols using lc 2010aht
1
HPLC Quantification of Compound X
2
HPLC Analysis of Phytochemicals
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Chromatographic conditions: HPLC ultraviolet detector (Shimadzu LC-2010AHT with LC Solution workstation); chromatographic column (SynergiTM 4 µm Hydro-RP 80 Å 250x4.6 mm, Phenomenex); mobile phase: acetonitrile 0.1% formic acid aqueous solution = 14.5: 85.5(v/v) (0–21 mins), acetonitrile : 0.1% formic acid aqueous solution = 35: 65 (v/v) (21.1–28 mins), acetonitrile : 0.1% formic acid aqueous solution = 14.5: 85.5 (v/v) (28.1–34 mins); flow rate 1.0 mL/min; wavelength 310 nm; injection volume 10 µL.
3
Formic Acid Analysis by HPLC
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A LC2010AHT liquid chromatograph (SHIMADZU, Japan) was used to analyze the formic acid. The chromatographic column was Hypersil BDS C18 (250 mm × 4.6 mm × 5 m), and the UV detector was set at 210 nm. The mobile phase was 0.02 mol/LKH2PO4 methanol with a volume ratio of 95:5, and the phosphoric acid was used to adjust the pH to approximately 2. The flow rate was set to 1.0 mL/min, and the column temperature was 30 °C.
4
Chiral Separation of Ketoprofen Esters
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Quantitative analysis of the samples was performed via HPLC through a CHIRALPAK column (Chiral AD-H, 5 μm, 4.6 mm × 250 mm; Daicel Chemical) using a Shimadzu LC-2010A HT apparatus equipped with a 254 nm UV detector. Hexane with 10% (v/v) isopropanol was employed as the mobile phase with a split flow rate of 0.5 mL/min. Retention times were as follows: (R)-ketoprofen vinyl ester, 12.24 min; (S)-ketoprofen vinyl ester, 13.00 min; (R)-ketoprofen, 25.18 min; and (S)-ketoprofen, 29.14 min.
5
GC/MS and Chiral GC Analysis Protocol
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GC/MS analyses
were performed on a Shimadzu GCMS-QP2010 equipped with a RTX-XLB column
(30 m × 0.25 mm × 0.28 μm) and a quadrupole mass analyzer.
Separation method: 1 μL injection, inj. temp.: 250 °C,
detector temp.: 220 °C. Gradient: column temperature set at 50
°C for 1 min, then to 280 °C at 30 °C/min, then to280
°C for 4 min. Total run time was 13.67 min. Enantiomeric excess
was determined by chiral gas chromatography (GC) using a Shimadzu
GC-2010 gas chromatograph equipped with a FID detector and a Agilent
CYCLOSIL-B column (30 m × 0.25 mm × 0.25 μm film).
Separation method for 4-methyl-4-(p-tolyl)oxazolidin-2-one (15 ): 1 μL injection, injector temp.: 200 °C, detector
temp: 300 °C. Gradient: column temperature set at 150 °C
for 0 min, then to 200 °C at 4.5 °C/min, then to 230 °C
at 1.5 °C/min, then to 245 °C at 15 °C/min for 8 min.
Separation method for 4-phenyloxazolidin-2-one (20 ):
1 μL injection, injector temp.: 200 °C, detector temp:
300 °C. Gradient: column temperature set at 150 °C for 0
min, then to 200 °C at 4.5 °C/min, then to 220 °C at
1 °C/min, then to 245 °C at 15 °C/min for 8 min. HPLC
analyses were performed using a Shimadzu LC-2010A-HT equipped with
a VisionHT-C18 reverse-phase column and a multidiode UV–VIS
detector. Injection volume: 10 uL. Flow rate: 1 mL/min. Gradient:
10% acetonitrile in water (0.1% TFA) for 3 min, then increased to
90% over 24 min.
were performed on a Shimadzu GCMS-QP2010 equipped with a RTX-XLB column
(30 m × 0.25 mm × 0.28 μm) and a quadrupole mass analyzer.
Separation method: 1 μL injection, inj. temp.: 250 °C,
detector temp.: 220 °C. Gradient: column temperature set at 50
°C for 1 min, then to 280 °C at 30 °C/min, then to280
°C for 4 min. Total run time was 13.67 min. Enantiomeric excess
was determined by chiral gas chromatography (GC) using a Shimadzu
GC-2010 gas chromatograph equipped with a FID detector and a Agilent
CYCLOSIL-B column (30 m × 0.25 mm × 0.25 μm film).
Separation method for 4-methyl-4-(p-tolyl)oxazolidin-2-one (
temp: 300 °C. Gradient: column temperature set at 150 °C
for 0 min, then to 200 °C at 4.5 °C/min, then to 230 °C
at 1.5 °C/min, then to 245 °C at 15 °C/min for 8 min.
Separation method for 4-phenyloxazolidin-2-one (
1 μL injection, injector temp.: 200 °C, detector temp:
300 °C. Gradient: column temperature set at 150 °C for 0
min, then to 200 °C at 4.5 °C/min, then to 220 °C at
1 °C/min, then to 245 °C at 15 °C/min for 8 min. HPLC
analyses were performed using a Shimadzu LC-2010A-HT equipped with
a VisionHT-C18 reverse-phase column and a multidiode UV–VIS
detector. Injection volume: 10 uL. Flow rate: 1 mL/min. Gradient:
10% acetonitrile in water (0.1% TFA) for 3 min, then increased to
90% over 24 min.
6
Quantifying Drug Encapsulation in Polymeric Nanoparticles
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For the purpose of evaluating the drug-loading material, following the disintegration of free drug, PTX-A10-3.2-PLGA NBs were completely dissolved in methanol using sonication. The PTX density of the solution was evaluated using high-performance liquid chromatography (HPLC, LC-2010AHt; Shimadzu, Kyoto, Japan). The drug encapsulation efficiency and the drug-loading were calculated via the following equations:
W1 represents the total drug amount in the PTX-A10-3.2-PLGA NBs, W2 represents the weight of the provided drug, and W3 represents the weight of the provided drug and NBs mixture. Each process was performed in triplicate.
W1 represents the total drug amount in the PTX-A10-3.2-PLGA NBs, W2 represents the weight of the provided drug, and W3 represents the weight of the provided drug and NBs mixture. Each process was performed in triplicate.
7
Catalytic Conversion of Corn Stalk Biomass
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The compositional analysis had been summarized in previous articles by our research group.17 (link) There are mainly 20.5% xylan, 31.6% glucan and 22.8% lignin in raw corn stalk and some other minor components such as glycan, extractive, ash etc. The catalytic reaction was performed in a 25 mL stainless-steel autoclave. In a typical process, 75 mg corn stalk was dispersed 10 mL GVL and mixed with 35 mg catalyst. The reactor was heated up to 190 °C for 50 min at 600 rpm. At the end of the reaction, the reactor was immediately cooled with cold water, and the reaction mixture was filtered, collected, and stored in refrigerator for subsequent analysis.
The samples were analyzed by high performance liquid chromatography (HPLC, LC-2010AHT, SHIMADZU) equipped with a C18 column (ZORBAX, Eclipse XDB-C18, Agilent) and an UV detector (SPD-10A, SHIMADZU) at 280 nm. A mixture solution of methanol and water (2/3 v/v) was used as the mobile phase (0.4 mL min−1), and the column temperature was maintained at 30 °C. Furfural and HMF yields for the various products have been calculated on a molar basis as follows:
The samples were analyzed by high performance liquid chromatography (HPLC, LC-2010AHT, SHIMADZU) equipped with a C18 column (ZORBAX, Eclipse XDB-C18, Agilent) and an UV detector (SPD-10A, SHIMADZU) at 280 nm. A mixture solution of methanol and water (2/3 v/v) was used as the mobile phase (0.4 mL min−1), and the column temperature was maintained at 30 °C. Furfural and HMF yields for the various products have been calculated on a molar basis as follows:
8
HPLC Enantiomeric Resolution of Hydroxychloroquine
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HPLC analyses were performed on a LC-2010A HT (Shimadzu, Kyoto Japan) consisting of an UV–Vis detector, automatic sampler and thermostatic column oven compartment. The resolution of HCQ enantiomers was achieved on a Chiralpak AD-H column (4.6 mm × 150 mm, particle size 5 μm) with column oven maintained at 20 °C. The mobile phase consisted: (A) n-hexane in the presence of 0.5% DEA, and (B) isopropanol. Two portions of mobile phases were mixed online (93:7, v/v) with an isocratic elution at a flow rate of 0.8 ml/min. The UV wavelength at 343 nm was set for detection with an injection volume of 10 μl.
9
HPLC Characterization of PRPE
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PRPE from Nebbiolo was characterized by using high performance liquid chromatography (HPLC, LC 2010 AHT equipped with Diode array SPD-M10AVP, Shimadzu corporation, Kyoto, Japan) technique. PRPE was filtered through 0.2 µm cellulose acetate filters (Target2TM, Thermo Scientific, Waltham, MA, USA) and analyzed using a C8 Luna column (150 × 4.6 mm; 5 µm particle size) from Phenomenex (Torrance, CA, USA) and operated at 25 °C. The mobile phases consisted of 2% (v/v) acetic acid in water, Mobile Phase A (MPA) and 0.5% acetic acid in water and acetonitrile (50:50 v/v), Mobile Phase B (MPB), by using the gradient program reported in Table 4 , at a flow rate of 0.8 mL/min and a total run time of 123 min [73 (link)].
The injection volume was 10 µL and the diode array operated in the wavelength range from 200 to 600 nm. Main Pph were identified through comparison with reference compounds. The quantitation of individual Pph was performed by using calibration curves of the corresponding reference compounds. Gallic acid (280 nm), quercetin (370 nm), rutin (355 nm), and malvidin-3-glucoside (520 nm) were dissolved in ethanol: water solution at the concentration of 1, 5, 10, 50 100, 150, and 200 µg/mL and analyzed with the same method reported above. The quantitation was performed by applying the standard calibration curve.
The injection volume was 10 µL and the diode array operated in the wavelength range from 200 to 600 nm. Main Pph were identified through comparison with reference compounds. The quantitation of individual Pph was performed by using calibration curves of the corresponding reference compounds. Gallic acid (280 nm), quercetin (370 nm), rutin (355 nm), and malvidin-3-glucoside (520 nm) were dissolved in ethanol: water solution at the concentration of 1, 5, 10, 50 100, 150, and 200 µg/mL and analyzed with the same method reported above. The quantitation was performed by applying the standard calibration curve.
10
Measurement of Plant Biochemical Markers
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SAMS activity in fresh samples of the basal 2 cm of cuttings was measured using an HPLC method, as described by Lindroth et al. [20] (link). Thermo-BioBasic SCX color spectrum column (4.6 mm×250 mm, 5 um), and ammonium formate solution was used for the mobile phase (pH = 4.0), with other parameters set as follows: flow velocity 1 ml/min, detection wavelength 254 nm, column temperature 25°C, sample amount 10 µl. ACS activity was assayed according to the spectrophotometry method described by Boller et al. [34] (link). Polyamines contents were assayed by HPLC (LC-2010AHT, Shimadzu, Japan), as described by Guan et al. [35] . A Kromasil reversed-phase C18 column (250 mm×4.6 mm) was used with a mobile phase consisting of 64% methanol, a sample amount of 10 µl, a flow velocity of 0.8 ml/min, a column temperature 25°C, and a detection wavelength of 254 nm. Ethylene production was measured using gas chromatography (Trace GC Ultra, America), as described by Li et al. [36] . A 2 M stainless steel packed column was used, with a hydrogen ion flame detector, a detector temperature of 150°C, an injection port temperature of 70°C, N2 at 40 kPa as the carrier gas, H2 gas at a flow velocity of 35 ml/min, and an air flow rate of 350 ml/min. Each 1 ml sample was injected using a syringe.
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