Eclipse ti2 e microscope

Manufactured by Yokogawa

The Eclipse Ti2-E microscope is a high-performance imaging system designed for advanced microscopy applications. It features a modular and configurable platform that supports a wide range of objectives, illumination sources, and detection systems. The core function of the Eclipse Ti2-E is to provide researchers with a versatile and powerful tool for high-resolution imaging and analysis of biological samples.

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Lab products found in correlation

Orca flash 4.0 v3 scmos camera, by Hamamatsu Photonics (2 mentions) Multilaser bed, by Toptica (2 mentions) Csu w1 spinning disk system, by Oxford Instruments (2 mentions) Du 888 camera, by Toptica (2 mentions) Csu w1, by Hamamatsu Photonics (1 mentions) Microamp optical adhesive film, by Thermo Fisher Scientific (1 mentions) P96 1.5h n, by Cellvis (1 mentions) Ixon ultra 888 emccd camera, by Oxford Instruments (1 mentions)

Close spelling variants of this product

Eclipse Ti2-E inverted microscope (5 citations) Eclipse Ti2-E (4 citations) Eclipse Ti2 microscope (7 citations) Eclipse Ti2 (4 citations) Ti2-E (6 citations) Ti2 microscope (5 citations)

7 protocols using eclipse ti2 e microscope

Live Cell Imaging Using Nikon Microscope

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Live cell imaging in Figures 1–3 was performed using a Nikon Eclipse Ti-2E microscope with a Yokogawa CSU-WI spinning disk system and a Photometrics Prime BSI sCMOS camera. A 100× 1.45-NA CFI Plan Apochromat Lambda objective was used.

Opalko H., Geng S., Hall A.R., Vavylonis D, & Moseley J.B. (2023). Design principles of Cdr2 node patterns in fission yeast cells. bioRxiv.

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Imaging Cells with Confocal Microscopy

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Cells fixed and stained with DAPI, FOXO1 (Alexa 488) and Phalloidin (Alexa 568) were imaged using a Nikon Eclipse Ti2-E microscope equipped with a Yokogawa confocal scanner unit (CSU-W1), solid state diode lasers (405, 488 and 568 nm) and a Hamamatsu ORCA-Flash4.0 V3 sCMOS camera. Cells were imaged for mean intensity analysis using a 40x objective (CFI Super FLUOR; 0.9 NA) and the higher resolution images were captured with the 60x objective (Plan Apo; 1.40 NA oil). DIC images were collected for a single plane, while fluorescence images were collected every micron for 10 microns (the bottom slices including those below the nucleus and several out of focus planes were excluded from downstream mean intensity analysis; 7 slices were used for the analysis).

AbuEid M., McAllister D.M., McOlash L., Harwig M.C., Cheng G., Drouillard D., Boyle K.A., Hardy M., Zielonka J., Johnson B.D., Hill R.B., Kalyanaraman B, & Dwinell M.B. (2021). Synchronous effects of targeted mitochondrial complex I inhibitors on tumor and immune cells abrogate melanoma progression. iScience, 24(6), 102653.

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Differential Interference Contrast Microscopy of Nucleosomes

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Differential interference contrast (DIC) microscopy was carried out on select NCP samples following 30 min acquisition of turbidity data. For H3(1–44) or H4(1–25)L22Y with 601 DNA or NCP, DIC microscopy was carried out immediately following sample preparation and mixing rather than following turbidity assay due to the timescale of turbidity loss. Imaging was carried out at room temperature in volumes of 75 μL per well. Samples were in black polystyrene with #1.5 cover glass 96-well plates (P96–1.5H-N, Cellvis, Mountain View, CA) that were pre-treated as described above in “Turbidity assay” and sealed with MicroAmp Optical Adhesive Film (ThermoFisher Scientific, Waltham, MA).
Microscopy was performed on a Nikon Eclipse Ti2-E microscope equipped with a Yokogawa confocal scanner unit (CSU-W1) with 20x (Plan Apo; 0.75 NA), 40x (CFI Super Fluor; 0.9 NA), and 100x (Plan Apo; 1.45 NA oil) objectives, as noted in the associated figure legends. The system has a Hamamatsu ORCA-Flash4.0 V3 sCMOS Camera with an 82% quantum efficiency chip and runs the NIS-Elements Advanced Research package software. The field of view for the camera is 2048 × 2044 pixels, with resolution of 326.07, 162.02, and 65.24 nm/pixel for 20x, 40x, and 100x objectives, respectively. Images were processed using Fiji and ImageJ version 2.1.0/1.53c [61 (link),62 (link)] with the BioFormats plugin [63 (link)].

Hammonds E.F., Harwig M.C., Paintsil E.A., Tillison E.A., Hill R.B, & Morrison E.A. (2022). Histone H3 and H4 tails play an important role in nucleosome phase separation. Biophysical chemistry, 283, 106767.

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Spinning Disk Microscopy Imaging

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Samples were imaged at 1-μm-thick Z displacements through 20× and 40× air and 60× oil objectives of a Nikon Eclipse Ti2-E microscope with a Yokogawa CSU-W1 spinning disk system coupled to an Andor DU-888 camera, and a Toptica multilaser bed. Settings remained unchanged between conditions.

Pardo-Pastor C, & Rosenblatt J. (2023). Piezo1 activates noncanonical EGFR endocytosis and signaling. Science Advances, 9(39), eadi1328.

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Microscopy Protocol for Detailed Imaging

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Samples were imaged using 20x air and 60x oil objectives of a Nikon
Eclipse Ti2-E microscope equipped with a Yokogawa CSU-W1 spinning disk system,
an Andor DU-888 camera, and a Toptica multi-laser bed. All settings were kept
constant between conditions.

Dwivedi V.K., Pardo-Pastor C., Droste R., Kong J.N., Tucker N., Denning D.P., Rosenblatt J, & Horvitz H.R. (2021). Replication stress promotes cell elimination by extrusion. Nature, 593(7860), 591-596.

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Imaging Microtubule Depolymerization in Worm Embryos

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Embryos from adult worms were dissected on a 22 × 50 mm coverslip (Catalog # 2975-225; Coring) in 10-µl of egg salts buffer (ESB) containing 15-µm polystyrene beads (Sigma-Aldrich) using two 22-gauge needles. For microtubule depolymerization assays, ESB was mixed with nocodazole to make a 20 µM nocodazole solution or 1% DMSO. Samples were then mounted onto plain 25 × 75 × 1 mm microscope slides (# 12-544-4; Fisher Scientific). Time-lapse images were acquired with an inverted Nikon Eclipse Ti2-E microscope with a Yokogawa confocal scanner unit (CSU-W1), piezo Z stage, and an iXon Ultra 888 EMCCD camera (Andor), controlled by Nikon Elements software. We used a 60×1.2 NA Plan Apochromat water-immersion objective to acquire 41 × 0.5-µm Z-stacks with 100 ms exposures every min for 10 min starting slightly prior to or during pronuclear meeting. 488-nm excitation (15% laser power) was recorded using 2 × 2 binning followed by DIC imaging (92.3% iris intensity) using 1 × 1 binning.

Rios M.U., Bagnucka M.A., Ryder B.D., Ferreira Gomes B., Familiari N.E., Yaguchi K., Amato M., Stachera W.E., Joachimiak Ł.A, & Woodruff J.B. (2024). Multivalent coiled-coil interactions enable full-scale centrosome assembly and strength. The Journal of Cell Biology, 223(4), e202306142.

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Immunofluorescence Imaging of DNA Damage Markers

Cited 1 time

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Immunofluorescence was performed as previously described (68 (link)). Images were taken using Nikon i90 confocal microscope or Nikon Eclipse Ti2-E microscope equipped with a Yokogawa confocal scanner (CSU-W1). Primary antibody dilutions were as follows: 1:50 for phospho-Chk1 (Ser345); 1:500 for ATR; and 1:1000 for γH2AX (Ser139) and insulin. All secondary antibody dilutions were used at 1:1000.

Yeo C.T., Stancill J.S., Oleson B.J., Schnuck J.K., Stafford J.D., Naatz A., Hansen P.A, & Corbett J.A. (2021). Regulation of ATR-dependent DNA damage response by nitric oxide. The Journal of Biological Chemistry, 296, 100388.

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