IHC was used to analyze the expression ofα-SMA, LN, FN, Type I and III collagen in cells. The primary antibody as follows were used in the present study: Anti-alpha smooth muscle Actin antibody (Abcam, USA, ab5694), Anti-Low-density lipoprotein receptor-related protein 10 antibody (Abcam, USA, Ab111288), Anti-Collagen I antibody (Abcam, USA, Ab34710), Anti-Collagen III antibody (Abcam, USA, Ab7778), Anti-Fibronectin antibody (Abcam, USA, Ab2413), Anti-Laminin antibody (Abcam, USA, Ab128053). The secondary antibody labeled with fluorochrome diluted in 10% NGS (Alexa Fluor 488-labeled Goat Anti-Rabbit IgG (H+L) (Biyuntian Biotechnology Co., Ltd., Shanghai, China) was used. All of the images were obtained by an Eclipse TE2000-S electron fluorescence microscope (Nikon, Japan) and analyzed by Image J software as described previously (Vayrynen et al., 2012 (link)).
Ab128053
Manufactured by Abcam
Sourced in United States
Ab128053 is a primary antibody that targets a specific protein. It can be used in various laboratory techniques to detect the presence and/or quantify the target protein.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ab128053
1
Immunohistochemical Analysis of ECM Proteins
2
ECM Composition Analysis of Decellularized Tissues
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Native thymi, decellularized tissues, and 3D scaffolds were fixed with 4% paraformaldehyde overnight at 4 °C, embedded in OCT (Tissue‐Tek) and cross‐sectioned (10 µm thick). Sections were rinsed with PBS and blocked with PBS/2% BSA. Tissues were then incubated overnight with monoclonal anti‐Collagen IV (ab6586, Abcam), Laminin (ab128053, Abcam) or fibronectin (ab2413, Abcam) primary antibodies. After rinsing in PBS, samples were incubated for 40 min at RT with Alexa Fluor 488, 546, or 647 secondary antibodies (Abcam). After rinsing in PBS, nuclei were stained with DAPI. Imaging was performed with a confocal microscope (Zeiss LSM 710).
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