Cd19 clone ebio1d3

Manufactured by Thermo Fisher Scientific

CD19 (clone eBio1D3) is a mouse monoclonal antibody that recognizes the human CD19 cell surface antigen. CD19 is a B-lymphocyte-restricted cell surface protein that is expressed from the pro-B phase of B-cell development through early plasma cell differentiation.

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EBio1D3 (13 citations) CD19 (eBio1D3, (7 citations) Anti-CD19–PE-Cy7 (clone eBio1D3 (1D3), (2 citations)

3 protocols using cd19 clone ebio1d3

Characterization of Tumor-Infiltrating Lymphocytes

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Single cell suspensions from spleens and lymph nodes were prepared as described.6 (link) TIL were isolated from pooled tumors as described.26 (link) All flow cytometry experiments were performed at least 3 times. Single cell suspensions were incubated with mouse Fc receptor binding inhibitor for 10 minutes before staining with antibodies to CD45 (clone 30-F11), CD3 (clone 145-2C11), CD4 (clone GK1.5), CD8 (clone 53-6.7), CD19 (clone eBio1D3), CD86 (clone GL1), CD11b (clone M1/70), Gr-1 (clone RB6-8C5), CD69 (clone H1.2F3), CD44 (clone IM9), CD62L (clone MEL-14), and CD11c (clone N418; all from eBioscience, San Diego, CA) for 30 minutes. Flow cytometry was performed using FACS Calibur (BD Biosciences) and the lymphocyte population was selected by gating CD45⁺ cells. The data were analyzed using Flow Jo software (Tree Star, Ashland, OR). For tetramer staining, 10 μL of PE labeled HLA-A*02:01 Human HPV16 E7 tetramer (NIH Tetramer Core Facility) was added to 200 μL mouse lymphocyte suspension (1×106 cells per tube). After incubation for 30 minutes, cells were centrifuged and resuspended in phosphate-buffered saline with 1% paraformaldehyde and then analyzed by flow cytometry. PE labeled HLA-A*02:01 human mesothelin tetramer (NIH Tetramer Core Facility) was used as control.

Dai M., Hellstrom I., Yip Y.Y., Sjögren H.O, & Hellstrom K.E. (2018). Tumor Regression and Cure Depends on Sustained Th1 Responses. Journal of Immunotherapy (Hagerstown, Md. : 1997), 41(8), 369-378.

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Multiparameter Flow Cytometry Immunophenotyping

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CD45R/B220 (clone RA3-6B2, 1:100, eBioscience), CD3 (145-2c11, 1:100, eBioscience), CD45.2 (clone 104, 1:100, eBioscience), CD4 (clone GK1.5, 1:100, eBioscience), CD8 (clone 53-6.7, 1:100, eBioscience), CD19 (clone eBio1D3, 1:100, eBioscience), CD5 (clone 53-7.3, 1:100, eBioscience), CD21 (clone eBio4E3, 1:100, eBioscience), CD43 (clone eBioR2/60, 1:100, eBioscience), IgD (clone 11–26, 1:100, eBioscience), Gr1 (clone RB6-8C5, 1:100, eBioscience), IgM (clone 11f41, 1:100, eBioscience), GL-7 (clone GL-7, 1:100, eBioscience), Mac1 (clone M1/70, 1:100, eBioscience).

Souroullas G.P., Fedoriw Y., Staudt L.M, & Sharpless N.E. (2017). Lkb1 deletion in murine B lymphocytes promotes cell death and cancer. Experimental hematology, 51, 63-70.e1.

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Immunofluorescence Staining of Salivary Glands

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Immunofluorescence (IF) staining was performed as previously described on formalin-fixed, paraffin-embedded (FFPE) labial salivary gland biopsies from patients with SS10 29 30 (link) and on murine SGs obtained from virus cannulated and control mice.30 (link)
The following antibodies were used: for mouse CD45 clone 30-F11, CD19 clone eBio1D3 and CD3e clone ebio500A2 (from eBiosciences) and for humans CD3 polyclonal rabbit or monoclonal mouse (Dako), CD20 clone L26 (Dako), CD138 and CD68 (Abd Serotech) and pS6 polyclonal rabbit (Cell signalling).

Nayar S., Campos J., Smith C.G., Iannizzotto V., Gardner D.H., Colafrancesco S., Pipi E., Kollert F., Hunter K.J., Brewer C., Buckley C.D., Bowman S.J., Priori R., Valesini G., Juarez M., Fahy W.A., Fisher B.A., Payne A., Allen R.A, & Barone F. (2018). Phosphatidylinositol 3-kinase delta pathway: a novel therapeutic target for Sjögren’s syndrome. Annals of the Rheumatic Diseases, 78(2), 249-260.

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