A 6-week-old female C57BL/6 (B6) and athymic nude mice (CrTac:NCr-Foxn1nu) were purchased from Taconic Biosciences (Derwood, Maryland, USA). NSG mice (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) were purchased from the Jackson Laboratory (Bar Harbor, Maine, USA). All mice were maintained under specific pathogen-free conditions at the Johns Hopkins University School of Medicine Animal Facility (Baltimore, Maryland, USA).
Nod cg prkdcscid il2rgtm1wjl szj nsg mice
Manufactured by Jackson ImmunoResearch
Sourced in United States, Montenegro
The NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice are a strain of immunodeficient mice developed for use in biomedical research. They exhibit severe combined immunodeficiency and lack of mature T cells, B cells, and functional natural killer cells.
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Lab products found in correlation
C57bl 6 tg tcratcrb 1100mjb j ot 1 mice, by Jackson ImmunoResearch (5 mentions)
C57bl 6 mice, by Jackson ImmunoResearch (5 mentions)
Living image software, by PerkinElmer (4 mentions)
B6.129s7 rag1tm1mom j, by Jackson ImmunoResearch (3 mentions)
Matrigel, by BD (3 mentions)
Nsg mice, by Jackson ImmunoResearch (3 mentions)
Nod cb17 prkdcscid ncrcrl nod scid mice, by Charles River Laboratories (3 mentions)
Ivis imaging system, by PerkinElmer (3 mentions)
Tryple express, by Thermo Fisher Scientific (3 mentions)
Pbs matrigel, by BD (3 mentions)
C57bl 6j mice, by Jackson ImmunoResearch (2 mentions)
Ivis spectrum in vivo imaging system, by PerkinElmer (2 mentions)
Pth 1 34, by R&D Systems (2 mentions)
Angiogenesis assay kit, by Merck Group (2 mentions)
Matrigel, by Corning (2 mentions)
D luciferin, by Biosynth (2 mentions)
Ivis spectrum, by PerkinElmer (2 mentions)
Crl cd1 icr mice, by Charles River Laboratories (2 mentions)
C57bl 6 crbntm1.1ble j, by Jackson ImmunoResearch (2 mentions)
Anti mouse cd45, by Thermo Fisher Scientific (2 mentions)
221 protocols using nod cg prkdcscid il2rgtm1wjl szj nsg mice
1
Mouse Strains for In Vivo Studies
2
Mouse Experiments Ethics and Protocols
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All mouse experiments were performed according to experimental protocols approved by the Animal Ethics Committee of City University of Hong Kong and the Animal Care and Use Committees of Boston Children’s Hospital . BALB/c mice were purchased from Chinese University of Hong Kong. BALB/c SCID mice, nude mice and NSG mice (NOD.Cg-Prkdcscid Il2rg tm1Wjl /SzJ) were purchased from the Jackson Laboratory (USA). All mice were monitored and bred in our facilities.
3
Genetic Manipulation of Vascular Endothelial Cells
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Vascular endothelial cadherin (VE-cadherin)-Cre transgenic mice (Stock No. 006137) [40 (link)] and Fbw7-floxed (Fbw7flox/flox) mice (Stock No. 017563) [24 (link)] were purchased from The Jackson Laboratory. VE-cadherin-Cre mice were intercrossed with Fbw7flox/flox mice to generate Fbw7flox/flox;VE-cadherin-Cre mice. Fbw7flox/flox;VE-cadherin-Cre mice were further bred with ROSAmT/mG mice (The Jackson Laboratory, Stock No. 007576) to generate triple-transgenic Fbw7flox/flox;VE-Cadherin-Cre;ROSAmT/mG mice. NSG mice (NOD.Cg-Prkdcscid;Il2rgtm1Wjl/SzJ, Stock No. 005557) were purchased from The Jackson Laboratory. Mice were housed in a controlled environment (22 °C, 12-h/12-h light/dark cycle) with free access to food and water. All mouse experimental protocols were approved by the Institutional Animal Care and Use Committee at Georgia State University and were in compliance with relevant ethical regulations. Genotyping for each strain was performed as described on The Jackson Laboratory website.
4
Generation of Genetically Modified Mice
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C57BL/6J (B6, H-2b) mice, CD45.1 mice and β2M-deficient mice were obtained from the Jackson Laboratory. Rag1−/−γc−/− mice were obtained by intercrossing B6.129S7-Rag1tm1Mom/J with NSG mice (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ; the Jackson Laboratory) and then backcrossing with B6 mice for at least eight generations. Mice lacking various genes were generated by injecting synthetic guide RNAs (gRNAs) with the Cas9 enzyme into pure C57BL/6J fertilized ovules and then transferring them into surrogate female mice. Ly49-deficient mice were screened by genomic PCR. The oligonucleotide sequences of primers were summarized in Supplementary Table 1 . The products were electrophoretically separated on gels and visualized using a ChampGel 5000 Gel Imaging System (Beijing Sage Creation, China). Guide RNAs for generating various mutant mice were summarized in Supplementary Table 2 . All mice were housed and bred in specific pathogen-free animal facilities at Tsinghua University. All experiments using mice were approved by the Animal Ethics Committee of Tsinghua University.
5
Mouse Models for Cancer Research
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C57BL/6 mice (C57BL/6NCrl; female, 6 to 8 weeks old) were purchased from Charles River Laboratories (USA). Athymic Nu mice [Crl:NU(NCr)-Foxn1nu; origin: Charles River Laboratories; female, 6 to 8 weeks old] and NSG mice (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ; origin: the Jackson Laboratory; female, 6 to 8 weeks old) were purchased from the Animal Study Core at the UNC Lineberger Comprehensive Cancer Center. These outbred Nu mice originated from Charles River Laboratories. Animals were maintained in the Division of Comparative Medicine [an Association for Assessment and Accreditation of Laboratory Animal Care International (AAALAC)–accredited experimental animal facility] under sterile environments at the UNC at Chapel Hill. All procedures involving experimental animals were performed by the protocols approved by the UNC Institutional Animal Care and Use Committee. All cesium-137 irradiation experiments were performed in a Gammacell 40 extractor cesium-137 research irradiator in collaboration with approved personnel from the Animal Study Core at the UNC Lineberger Comprehensive Cancer Center. In vivo studies were performed in collaboration with the Animal Study Core at the UNC Lineberger Comprehensive Cancer Center.
6
Xenograft of Mouse Kidney Capsule
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NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ mice (NSG) were obtained from Jackson Laboratories. Mice used in this study were maintained according to protocols approved by the University of California, San Francisco Committee on Laboratory Animal Resource Center. Mouse kidney capsule grafts have been described previously (Russ et al., 2015 (link)).
7
Breeding and Maintaining NSG Mice
8
Preclinical Evaluation of CD19-Targeted CAR T Cells
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Animal studies were performed in strict accordance with the regulations of the Johns Hopkins Animal Care and Use Committee and Omeros’ Animal Care and Use Committee. NOD.Cg-PrkdcscidIL-2rgtm1Wjl/SzJ mice (NSG) (the Jackson Laboratory, Bar Harbor, ME, USA) mice were injected either intravenously or subcutaneously with CD19+ Nalm6 tumor cells (DSMZ, Braunschweig, Germany) that were engineered as per the manufacturer’s protocol to express enhanced green fluorescent protein (eGFP) and fLuc (PerkinElmer, Hopkinton, MA, USA) (Nalm6-eGFP-fLuc). In the Nalm6 intravenously administered model, tumor engraftment was allowed to occur for 4 days and verified using BLI. On day 4, mice received a single intravenous injection of mock, CD19-EGFRt, or CD19-tPSMA(N9del) CAR T cells. In the Nalm6 subcutaneously disseminated model, spontaneous metastatic lesions develop. Tumor engraftment was verified using BLI. On day 21 after tumor implantation, mice received a single intravenous injection of mock, CD19-EGFRt, or CD19-tPSMA(N9del) CAR T cells.
9
Transplantation of hESC-Derived Cells
10
Xenograft Establishment in NSG Mice
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NOD.Cg-Prkdcscid Il2rgtm1wjl /SzJ mice (NSG mice) were obtained from The Jackson Laboratory (Bar Harbor, ME, USA). All animal experiments were approved by the Seoul National University Institutional Animal Care and Use Committee (IACUC, SNU 15112–3-4). A total of 10 female NSG mice were used. Orthotropic xenografts were established via injection of 1 × 106 shNSDHL MDA-MB-231 cells (n = 5) or shControl cells (n = 5) mixed with Matrigel (BD Biosciences) into the fat pad of the 4th mammary gland of 5-week old mice. After injection of tumor cells, primary tumor volume was measured weekly using digital calipers and a modified ellipsoidal formula (volume = 1/2(length×width2)) [17 (link)].
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