Perchloric acid pca

Manufactured by Avantor

Sourced in United Kingdom, Canada, United States

Perchloric acid (PCA) is a strong inorganic acid with the chemical formula HClO4. It is a colorless, fuming liquid that is soluble in water and organic solvents. Perchloric acid is used as a laboratory reagent and in various industrial applications.

Automatically generated - may contain errors

Lab products found in correlation

4 protocols using perchloric acid pca

HPLC Analysis of Glutathione Redox Status

Check if the same lab product or an alternative is used in the 5 most similar protocols

GSH, GSSG, O-pthaldialdehyde (OPA), N-ethylmaleimide (NEM), ethylenediaminetetraacetic acid (EDTA) and sodium hydrogen phosphate (NaH₂PHO₄) were purchased from (Sigma-Aldrich, Poole, UK). HLPC-grade acetonitrile and methanol were obtained from Fisher Scientific (Leicester, UK) and used without further purification. Perchloric acid (PCA) was obtained from VWR International (Loughborough, England). Chromacol autosampler vials (300 µL) were obtained from Thermo Scientific (Langerwehe, Germany). Chromatographic-grade water was generated from Milli-Q Advantage System^TM (Millipore, Watford, UK) and filtered through Whatman Filter Paper (Fisher Scientific, Loughborough, UK). The NaH₂PHO₄ mobile phase solvent was degassed and filtered through a 0.45-µm Whatman Filter Paper. GSH and GSSG standard solutions were prepared fresh each day. O-pthaldialdehyde (OPA) solution in methanol was prepared every 4 hrs and NEM was prepared just before use. A summary of the whole procedure is detailed in Figure 11.

Nuhu F., Gordon A., Sturmey R., Seymour A.M, & Bhandari S. (2020). Measurement of Glutathione as a Tool for Oxidative Stress Studies by High Performance Liquid Chromatography. Molecules, 25(18), 4196.

+ Open protocol

+ Expand

Quantification of Thymidine and 2'-Deoxyuridine

Check if the same lab product or an alternative is used in the 5 most similar protocols

Thymidine, 2’-deoxyuridine and formic acid (LC-MS grade) were obtained from Sigma Aldrich (Poole, UK). Internal standards (IS) Thymidine-1’,2’,3’,4’,5’-¹³C₅ and 2’-deoxyuridine-1’,2’,3’,4’,5’-¹³C₅ were from Toronto Research Chemicals (Toronto ON, Canada) and 70% perchloric acid (PCA) was obtained from VWR (Lutterworth, UK). Methanol (HPLC grade) was supplied by Rathburn (Walkerburn, Scotland). Deionized water was prepared in-house (<1.0 µS/cm). Analyte-free dialyzed human plasma (with K₂EDTA) was obtained from TCS Biosciences Ltd (Buckingham, UK). Analyte-free human plasma containing K₃EDTA, lithium heparin, analyte-free human serum from individual donors and whole blood (for the preparation of hemolyzed plasma) were obtained from Biological Speciality Corporation (Colmar, PA, USA). Hyperlipidaemic plasma containing 247 mg/dL cholesterol was supplied by Seralab (Haywards Heath, UK). Urine was obtained from in-house healthy volunteers.

Kipper K., Hecht M., Antunes N.J., Fairbanks L.D., Levene M., Kalkan Uçar S., Schaefer A., Blakely E.L, & Bax B.E. (2020). Quantification of Plasma and Urine Thymidine and 2’-Deoxyuridine by LC-MS/MS for the Pharmacodynamic Evaluation of Erythrocyte Encapsulated Thymidine Phosphorylase in Patients with Mitochondrial Neurogastrointestinal Encephalomyopathy. Journal of Clinical Medicine, 9(3), 788.

+ Open protocol

+ Expand

Determination of Aminothiol Disulfides

Check if the same lab product or an alternative is used in the 5 most similar protocols

In general, chemicals used in this study were commercially available and at least of analytical reagent grade. Sodium hydrogen phosphate dihydrate, sodium dihydrogen phosphate dihydrate, tris(2-carboxyethyl)phosphine (TCEP), HPLC-gradient grade acetonitrile (ACN), trichloroacetic acid (TCA), Cys-Gly, symmetrical disulfides of particular aminothiols, namely homocystine (Hcy₂), cystine (Cys₂) and oxidized GSH (Glu₂) were from Sigma-Aldrich, (St. Louis, MO, USA). Hydrochloric acid, sodium hydroxide and perchloric acid (PCA) were from J.T. Baker (Deventer, The Netherlands). 2-Chloro-1-methylquinolinium tetrafluoroborate (CMQT)^{17 (link)} and HPPTCA¹⁶ were prepared in our laboratory as previously described. Deionized water was produced in our laboratory. Commercially available 50 mg vitamin B6 tablets from Teva Pharmaceuticals (Cracow, Poland), containing active substance pyridoxine hydrochloride, were used.

Piechocka J., Wyszczelska-Rokiel M, & Głowacki R. (2023). Simultaneous determination of 2-(3-hydroxy-5-phosphonooxymethyl-2-methyl-4-pyridyl)-1,3-thiazolidine-4-carboxylic acid and main plasma aminothiols by HPLC–UV based method. Scientific Reports, 13, 9294.

+ Open protocol

+ Expand

Acetyl-CoA Synthesis and Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

All solutions were prepared in Millipore water (Milli-Q system, Billerica, MA, USA). Aqueous solutions (72%) of perchloric acid (PCA) and HPLC grade acetonitrile were obtained from JT Baker (Phillipsburg, NJ, USA); the trilithium salt of acetyl-CoA, the sodium salt hydrate of CoA, sodium acetate, monosodium phosphate, 85% aqueous phosphoric acid, oxaloacetic acid, citrate synthase (from porcine heart; ammonium sulfate suspension; >1000 U/mg), acetyl phosphate, Tris HCl, and dithiothreitol (DTT) were obtained from Sigma Aldrich (Saint Louis, MO, USA); phosphotransacetylase (lyophilized preparation from Bacillus stearothermophilus, ~1000 U/mg solid) was obtained from Boehringer Mannheim (Ridgefield, CT, USA); Dulbecco Modified Eagle’s Medium (DMEM) was obtained from VWR Life Sciences (Radnor, PA, USA); fetal bovine serum (FBS) was obtained from Gemini Bio-Products (West Sacramento, CA, USA); and, the Bio-Rad DC™ protein assay kit was obtained from Bio-Rad (Hercules, CA, USA). Nylon membrane filters with a diameter of 47 mm and a pore size of 0.2 µm that were used for mobile phase filtering and degassing were obtained from Pall Life Science (Port Washington, NY, USA).

Shurubor Y.I., D’Aurelio M., Clark-Matott J., Isakova E.P., Deryabina Y.I., Beal M.F., Cooper A.J, & Krasnikov B.F. (2017). Determination of Coenzyme A and Acetyl-Coenzyme A in Biological Samples Using HPLC with UV Detection. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(9), 1388.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!