Optical measurement, including the confocal imaging, the fluorescence spectrum, and antibunching experiments, was based on our home-built confocal microscope, as shown in Fig. S15. A continuous-wave 488 nm laser was used for excitation. The laser was focused onto the sample using a high-numerical-aperture (NA = 0.95, Olympus) objective lens. The FWHM of the focal spot is 339 nm (Fig. S16). A polariser combined with a half-wave plate was used to control excitation power. For PL mapping and position, an X-Y-Z piezoelectric stage (PI instrument) was used. The collected fluorescence was filtered using a 500 nm dichroic mirror and an additional long-pass filter (Thorlabs FELH0500 or FELH0550). The signal was split by a beam splitter in the ratio of 30:70, and coupled into a grade-index fiber. One part of the signal was directed into a spectrometer (Princeton instruments) for collecting PL spectra, while the other part was directed into the two avalanche photodiodes (Excelitas, Dark count rate: around 80 Hz; Quantum efficiency: around 70% at 650 nm) for autocorrelation measurements. The fibre aperture serves as a confocal pinhole. Antibunching measurements were done using a time-correlated single-photon counting module (PicoHarp 300, PicoQuantum). The g2(t) data were not corrected for background luminescence.
Felh0500
Manufactured by Thorlabs
Sourced in Germany
The FELH0500 is a Fiber-Coupled External-Cavity Laser Head produced by Thorlabs. It is designed to provide a focused output beam from a single-mode optical fiber.
Automatically generated - may contain errors
Lab products found in correlation
La4148, by Thorlabs (2 mentions)
La4052, by Thorlabs (2 mentions)
Lmu15x, by Thorlabs (2 mentions)
Spectrometer, by Teledyne (1 mentions)
Felh0550, by Thorlabs (1 mentions)
Ixon ultra 897, by Oxford Instruments (1 mentions)
Ti2 a inverted microscope, by Nikon (1 mentions)
Fesh0450, by Thorlabs (1 mentions)
Ldh d c 375, by PicoQuant (1 mentions)
Lc100, by Thorlabs (1 mentions)
Acl25416u a, by Thorlabs (1 mentions)
Fes0500, by Thorlabs (1 mentions)
La1422 a, by Thorlabs (1 mentions)
Md499, by Thorlabs (1 mentions)
Cs2100m, by Thorlabs (1 mentions)
Apd430a2, by Thorlabs (1 mentions)
Nf533 17, by Thorlabs (1 mentions)
Ff01 607 70, by IDEX Corporation (1 mentions)
7 protocols using felh0500
1
Multimodal Optical Characterization of Samples
2
Fluorescence Microscopy Experimental Setup
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Fluorescence
microscopy experiments were performed using a Nikon Ti2-A inverted
microscope. The samples are placed on the microscope’s table,
which was applied with a custom-made box to allow measurements under
inert conditions. The excitation light was from a 373 nm collimated
free-beam laser diode (LDH-D-C-375, PicoQuant), passing a clean-up
filter (370/36 BrightLine HC, Semrock) and a lambda fourth plate (355
nm, Edmund Optics). The beam was expanded using a 10× UV beam
expander (BE10-UVB, Thorlabs, Inc.) and then focused on the back-focal
plane of the objective to enable far-field microscopy. It entered
the microscope through the backside port and was mirrored to the sample
stage via a dichroic mirror (zt 375 RDC, Chroma). Emitted light from
the sample was collected by the objective and passed the dichroic
mirror to be led to a side port of the microscope. Here, it was spectrally
separated into two parts using color filters (FESH0450 and FELH0500,
Thorlabs) and a dichroic mirror (zt 514 RDC, Chroma) mounted on an
Optosplit II (Acal BFi Germany GmbH). The two resulting images represented
the wavelength regimes. The image detection was done using a back-illuminated
CCD camera (iXon Ultra 897, Andor). Time-resolved measurements were
realized by taking a series of images and subsequent post-procession
of the data with a self-written evaluation script.
microscopy experiments were performed using a Nikon Ti2-A inverted
microscope. The samples are placed on the microscope’s table,
which was applied with a custom-made box to allow measurements under
inert conditions. The excitation light was from a 373 nm collimated
free-beam laser diode (LDH-D-C-375, PicoQuant), passing a clean-up
filter (370/36 BrightLine HC, Semrock) and a lambda fourth plate (355
nm, Edmund Optics). The beam was expanded using a 10× UV beam
expander (BE10-UVB, Thorlabs, Inc.) and then focused on the back-focal
plane of the objective to enable far-field microscopy. It entered
the microscope through the backside port and was mirrored to the sample
stage via a dichroic mirror (zt 375 RDC, Chroma). Emitted light from
the sample was collected by the objective and passed the dichroic
mirror to be led to a side port of the microscope. Here, it was spectrally
separated into two parts using color filters (FESH0450 and FELH0500,
Thorlabs) and a dichroic mirror (zt 514 RDC, Chroma) mounted on an
Optosplit II (Acal BFi Germany GmbH). The two resulting images represented
the wavelength regimes. The image detection was done using a back-illuminated
CCD camera (iXon Ultra 897, Andor). Time-resolved measurements were
realized by taking a series of images and subsequent post-procession
of the data with a self-written evaluation script.
3
Automated Paper-Based Fluorescence Detection
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On-field experiments were realized thanks to carry-on detection equipment. It consists in two blue LEDs (Thorlabs – M490L3–490 nm) combined with a first lense (Thorlabs ACL25416U-A), two filters (Thorlabs FES0500 – Thorlabs M497-16) and a second lense (Thorlabs LA1422-A) to light the paper. The signal emitted by the biological reaction on paper is collected by a linear camera (Thorlabs LC-100) through a first lense (Thorlabs ACL3026-A), a FITC dichroic filter (Thorlabs MD499), a filter (Thorlabs FELH0500) and a second lense (Thorlabs ACL25416U-A). The paper device is positioned on a chip holder heated by a PTC heater (DBK HP05), controlled by a platine thermic element (RS Components PT 1000ohms) and a temperature controller (Carel IR33). A home-made Microsoft Office 2007 macro program enables to extract data from the linear camera recording software (Splicco), to detect the position of the maximum fluorescence intensity for each paper rectangular area and to monitor the mean intensity around each maximum over time.
4
Ultraviolet Light Communication Setup
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A UVC LED (LG Innotek, LEUVA66H70HF00) was modulated through a bias tee (Mini-Circuits, ZFBT-4R2GW-FT+) and connected to a BERT transmitter (Anritsu, ME522A). Two plano-convex lenses (Thorlabs, LA4148 and LA4052) and an objective lens (Thorlabs, LMU15X) were used to guide the incoming light onto the Si-based APD. In the case of CsPbBr3 perovskite NCs, a 500-nm LP filter (Thorlabs, FELH0500) was added. For the UVC LED only, UV ND filters (Thorlabs, NDUV10A and NDUV06A) were added to reduce the light power and maintain it below the saturation power of the APD. The signal received from the APD was analysed by the BERT receiver (Anritsu, ME522A). The eye diagrams were simultaneously captured using a digital communication analyser (Agilent, 86100C Infiniium DCA-J Wideband Oscilloscope).
5
Intrinsic Optical Imaging of Olfactory Bulb
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Intrinsic optical imaging of the olfactory bulb18 (link) was done using a pair of back-to-back SLR lenses with a 50 mm f/1.4 lens used as objective and a second lens Tamron AF 90 mm f/2.8 Di SP AF/MF 1:1 Macro Lens coupled to an sCMOS camera (CS2100M, Thorlabs). The camera was fitted with a long pass filter with a cut-on wavelength of 500 nm (FELH0500, Thorlabs). This setup resulted in a resolution of 3.3 µm per pixel. White light from a flashlight was used to find the surface of the olfactory bulb. The imaging plane was set between 200 and 250 µm below the vasculature on the surface of the bulb. Single odors and odor mixtures were presented for 9 s randomly interleaved.
6
Ultrafast Optical Characterization of CsPbBr3 Nanocrystals
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The setup consisted of a 375-nm LD (Thorlabs, L375P70MLD) mounted on a thermoelectric cooler (TEC). The output signal from a vector network analyser (Agilent, E5061B) was connected to the LD. The light was guided through UV plano-convex lenses (Thorlabs, LA1951A & Edmund Optics, No. 36–689) into the integrating sphere. The light re-emitted from CsPbBr3 NCs was filtered by a 500-nm LP filter (Thorlabs, FELH0500) and passed through two plano-convex lenses (Thorlabs, LA4148 and LA4052) and an objective lens (Thorlabs, LMU15X) before being collected by a Si-based APD (Thorlabs, APD430A2). The APD was connected to the vector network analyser. The vector network analyser was pre-calibrated with an E-calibration module (Agilent, 85093–60010) before the experiment.
7
Laser-Based Stimulation and Imaging of HEK293 Cells
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For HEK293 stimulation, the quasi-cw OPO output at 1,440 nm was loosely focused with a 1-m-focal-length lens into a spot 670 μm in diameter (Fig. 2b ), providing a uniform irradiation of a large number of TRPA-expressing cells close to the beam centre. Glass-bottom Petri dishes with cultured cells were placed on a stage of an upright multiphoton microscope (Thorlabs) equipped with XLUMPLFLN objective (NA1.05, Olympus). Continuous-wave laser sources with wavelengths of 473 and 532 nm and an average power up to 50 mW were used for R-GECO1, EGFP, GCaMP6s and tdTomato visualization. Fluorescence from EGFP and GCaMP6s was filtered with an FELH0500 (Thorlabs) low-frequency filter and an FF01-510/42 (Semrock) bandpass filter. The signal from R-GECO1.1 and tdTomato was filtered with a NF533-17 (Thorlabs) notch filter and an FF01-607/70 (Semrock) bandpass filter. Fluorescence images were recorded using a cooled CCD camera 4070M-GE-TE (Thorlabs) with 4 × 4 binning and a 900-ms exposure.
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