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Alt activity assay kit

Manufactured by Merck Group
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The ALT activity assay kit is a laboratory tool designed to measure the activity of the enzyme alanine aminotransferase (ALT) in biological samples. ALT is an enzyme found in various tissues, and its measurement can provide information about liver function and health. The kit includes the necessary reagents and protocols to perform the ALT activity assay.

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Close spelling variants of this product

ALT Activity Assay (16 citations) ALT kit (7 citations) ALT activity kit (3 citations) Activity Assay Kits (2 citations)

31 protocols using alt activity assay kit

1

Whole Blood Analysis and Liver Function in Mice

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For whole blood analysis, mice were bled from the facial vein using a lancet. Blood was collected in EDTA tubes to prevent clotting (BD Biosciences), briefly vortexed and whole blood analysis was carried out using an ACT diff analyzer (Beckman Coulter) immediately after blood collection. For each blood sample, two analytical readings were taken and averaged. ALT Activity Assay kit (MAK052, Sigma-Aldrich) and AST Activity Assay kit (MAK055, Sigma-Aldrich) were used to perform liver function tests.
Rathore A.P., Mantri C.K., Tan M.W., Shirazi R., Nishida A., Aman S.A., Morrison J, & St. John A.L. (2021). Immunological and Pathological Landscape of Dengue Serotypes 1-4 Infections in Immune-Competent Mice. Frontiers in Immunology, 12, 681950.
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2

Liver and Muscle ALT and LDH Assay

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Fifty milligrams of liver and gastrocnemius muscle were homogenized in 500 μL of ALT or LDH Assay buffer and centrifuged at 10000 × g for 15 min at 4°C. ALT and LDH activities were determined using an ALT Activity Assay Kit (Sigma, Madrid, Spain) and LDH Activity Assay Kit (Sigma, Madrid, Spain), respectively.
Mariné-Casadó R., Domenech-Coca C., del Bas J.M., Bladé C., Arola L, & Caimari A. (2018). The Exposure to Different Photoperiods Strongly Modulates the Glucose and Lipid Metabolisms of Normoweight Fischer 344 Rats. Frontiers in Physiology, 9, 416.
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3

Quantifying Plasma Lipids and Enzymes

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Total plasma cholesterol and triacylglycerol were measured with commercially available colorimetric assays (Fluitest, Analyticon, Lichtenfels, Germany) according to the manufacturer’s protocol. The activity of hepatic transaminases alanine aminotransferase (ALT) in the serum was determined with a commercially available fluorometric assay kit (ALT Activity Assay Kit, Sigma-Aldrich) according to the manufacturer’s instructions.
Wüpper S., Fischer A., Lüersen K., Lucius R., Okamoto H., Ishida Y., Terao K, & Rimbach G. (2019). High Dietary Kuding Tea Extract Supplementation Induces Hepatic Xenobiotic-Metabolizing Enzymes—A 6-Week Feeding Study in Mice. Nutrients, 12(1), 40.
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4

Serum AST and ALT Quantification

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Serum was separated from HR-1 mouse blood samples and measured using a commercial enzyme-linked immunosorbent assay (ELISA) kit to measure the activity of AST and ALT. The AST activity assay kit (MAK055, Sigma Aldrich, St. Louis, MO, United States) and ALT activity assay kit (MAK052, Sigma Aldrich) were used according to the manufacturer’s instructions.
Gu M.J., Lee H.W., Yoo G., Kim D., Choi I.W., Kim Y, & Ha S.K. (2023). Protective effect of Schizonepeta tenuifolia Briq. ethanolic extract against UVB-induced skin aging and photodamage in hairless mice. Frontiers in Pharmacology, 14, 1176073.
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5

ZIKV Infection Treatment Protocol

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3- to 4-week-old female BALB/c orA129 mice were treated with 25HC (50 mg/kg) or the HβCD vehicle intraperitoneally (i.p.) 12 hr prior to infection i.p. with ZIKV strain GZ01/2016. The treatment was continued daily for seven days. Mice were observed daily for signs of illness or morbidity until 21 dpi. The survival rate was evaluated using the Log-rank (Mantel-Cox) test. Viral RNA in serum at 4 and 7 dpi and that in brain at 7 dpi were determined by qRT-PCR. The alanine aminotransferase (ALT) activity in serum at 7 dpi was detected using ALT activity assay kit (Sigma).
Li C., Deng Y.Q., Wang S., Ma F., Aliyari R., Huang X.Y., Zhang N.N., Watanabe M., Dong H.L., Liu P., Li X.F., Ye Q., Tian M., Hong S., Fan J., Zhao H., Li L., Vishlaghi N., Buth J.E., Au C., Liu Y., Lu N., Du P., Qin F.X., Zhang B., Gong D., Dai X., Sun R., Novitch B.G., Xu Z., Qin C.F, & Cheng G. (2017). 25-Hydroxycholesterol Protects Host against Zika Virus Infection and Its Associated Microcephaly in a Mouse Model. Immunity, 46(3), 446-456.
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6

Serum Biomarkers for Health Assessment

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Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH) activities, and high mobility group box 1 (HMGB1) concentration were measured using the ALT activity assay kit (MAK052; Sigma–Aldrich, St. Louis, MO, USA), AST activity assay kit (MAK055; Sigma–Aldrich), LDH activity assay kit (MAK066; Sigma–Aldrich), and mouse HMGB1 ELISA kit (NBP2-62767; Novus Biologicals, Littleton, CO, USA) according to the manufacturer's protocols, respectively.
Zhu Y., Lei L., Wang X., Chen L., Li W., Li J., Zhao C., Du X., Song Y., Gao W., Liu G, & Li X. (2023). The E3 ubiquitin ligase NEDD4-1 protects against acetaminophen-induced liver injury by targeting VDAC1 for degradation. Acta Pharmaceutica Sinica. B, 13(4), 1616-1630.
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7

Metabolic Biomarker Analysis in Liver Samples

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Serum glucose and triglyceride levels were measured using the Accutrend Plus meter (Roche Diagnostics, Indianapolis, IN). Serum β-hydroxybutyrate and liver ALT levels were measured using the β-hydroxybutyrate Assay Kit and ALT Activity Assay Kit, respectively (Sigma-Aldrich, Saint Louis, MO). Lipids were extracted from the liver samples by the Bligh/Dyer method and analyzed by gas-liquid chromatography as previously described (Zadravec et al., 2010 (link)).
Rando G., Tan C.K., Khaled N., Montagner A., Leuenberger N., Bertrand-Michel J., Paramalingam E., Guillou H, & Wahli W. (2016). Glucocorticoid receptor-PPARα axis in fetal mouse liver prepares neonates for milk lipid catabolism. eLife, 5, e11853.
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8

Serum Analysis for Liver Function

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Serums were collected and stored at −80°C until measured. Serum ALT activity was measured using an ALT Activity Assay kit (MAK052, Sigma-Aldrich, USA) according to the manufacturer’s instructions. The plasma concentrations of insulin were determined using an insulin enzyme-linked immunosorbent assay (ELISA) commercial kit (RayBiotech, USA) according to the manufacturer’s instructions. The hepatic TG content was measured using the Triglyceride Assay Kit (Abcam, Cambridge, UK) according to the manufacturer’s instructions.
Zhou Y., Liu Z., Lynch E.C., He L., Cheng H., Liu L., Li Z., Li J., Lawless L., Zhang K.K, & Xie L. (2020). Osr1 regulates hepatic inflammation and cell survival in the progression of non-alcoholic fatty liver disease. Laboratory investigation; a journal of technical methods and pathology, 101(4), 477-489.
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9

Biochemical Marker Evaluation Protocol

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Urea (Sigma Catalog # MAK179), creatinine (Sigma Catalog # MAK080), Alanine Transaminase (ALT) Activity Assay Kit (Sigma Catalog # MAK052), Aspartate Aminotransferase (AST) Activity Assay Kit (Sigma Catalog #MAK055), alkaline phosphatase (ALP) Activity Assay Kit (Sigma Catalog #MAK089) and γ-Glutamyltransferase (GGT) Activity Assay Kit (Sigma Catalog #MAK089), were used according to the manufacturer's instructions.
, & Abd El-Kaream S.A. (2019). Biochemical and biophysical study of chemopreventive and chemotherapeutic anti-tumor potential of some Egyptian plant extracts. Biochemistry and Biophysics Reports, 18, 100637.
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10

Colorimetric ALT Activity Assay

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Cells were grown to 80% confluency and 1 × 106 cells were collected as pellets in duplicates for the assay. Colorimetric assay to measure alanine aminotransferase activity in the cell lines was performed using the ALT activity assay kit from Sigma (MAK052-1KT) according to the manufacturer’s instructions. Zebrafish Liver cell line was used as positive control.
Krishnan J., Wang Y., Kenzior O., Hassan H., Olsen L., Tsuchiya D., Kenzior A., Peuß R., Xiong S., Wang Y., Zhao C, & Rohner N. (2022). Liver-derived cell lines from cavefish Astyanax mexicanus as an in vitro model for studying metabolic adaptation. Scientific Reports, 12, 10115.
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