The Cell Counting Kit −8 (CCK-8) was obtained from BioVision (Exton, PA). Cells were seeded into 96-well plates and incubated for 24 hours in a humidified incubator. Afterwards, CCK-8 working solution plus serum-free culture medium (1:10) was added to the plates (100 µL/well) and incubated for 0, 12, 24, and 48 h. The plate was incubated for 1 hour in the incubator. The optical density 450 nm (OD 450) was measured using a microplate reader (Molecular Devices, San Jose, CA, USA).
Cell counting kit 8 (cck8)
Manufactured by Abcam
Sourced in United Kingdom, United States, China
The Cell Counting Kit 8 is a colorimetric assay for the quantification of cell viability and cytotoxicity. It uses the water-soluble tetrazolium salt WST-8, which is reduced by dehydrogenases in living cells to produce a yellow-colored formazan dye. The amount of the formazan dye generated is directly proportional to the number of living cells.
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Lab products found in correlation
Microplate reader, by Thermo Fisher Scientific (6 mentions)
Elx800 microplate reader, by Agilent Technologies (3 mentions)
Nse assay kit, by Jiangsu Meimian (3 mentions)
Microplate reader, by Agilent Technologies (2 mentions)
96 well plate, by Corning (2 mentions)
Spectramax i3x, by Molecular Devices (2 mentions)
Lsm 710, by Zeiss (2 mentions)
Microplate reader, by Molecular Devices (1 mentions)
Rat t stim, by BD (1 mentions)
2 mercaptoethanol, by Merck Group (1 mentions)
Microplate reader, by Bio-Rad (1 mentions)
Edu assay, by Abcam (1 mentions)
Synergy 2, by Agilent Technologies (1 mentions)
Mission predesigned sirna, by Merck Group (1 mentions)
Countess 2, by Thermo Fisher Scientific (1 mentions)
Victor x4, by PerkinElmer (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
6 well plate, by Corning (1 mentions)
Synergy 2 multi mode microplate reader, by Agilent Technologies (1 mentions)
Cytotoxicity detection kit, by Roche (1 mentions)
68 protocols using cell counting kit 8 (cck8)
1
Cell Viability Assay using CCK-8
2
Mast Cell Histamine Release Assay
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The MC/9 mouse mast cells were gained from the American Type Culture Collection (Manassas, VA, USA). The cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 0.05 mM 2-mercaptoethanol (Sigma, Seoul, Korea), 10% rat T-STIM (Becton Dickinson, Franklin Lakes, NJ, USA), 2 mM L-glutamine, and 10% fetal bovine serum (FBS). The viability from the cells was evaluated using a Cell Counting Kit-8 (BioVision, Milpitas, CA, USA). Histamine concentrations in the cell supernatant were measured using a histamine immunoassay kit (Oxford Biomedical Research, Oxford, MI, USA). The cells were pretreated with various concentration of extracts before 30 min. Then, compound 48/80 (25 µg/mL) was treated and the cells were incubated for an additional 30 min. After the reaction was stopped, the optical density was measured at 650 nm using a microplate reader (BioRad, Hercules, CA, USA).
3
Evaluating Galangin Cytotoxicity with CCK8
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We evaluated the galangin cytotoxicity using the Cell Counting Kit 8 (CCK8, Abcam) as previously described [19 (link)]. In brief, the cells were seeded into 96-well plates in the presence of various galangin concentrations for 24, 48, and 72 h. Ten μL of CCK-8 reagent was added to each well before incubation for two hours. The absorbance was measured at 450 nm. The EdU assay was performed following the manufacturer’s protocol (Abcam).
4
Cell Viability Assay with CCK-8
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Cell viability was assessed using a CCK-8 (Cell Counting Kit 8, Abcam, Cambridge, UK) assay. After transfection for 48 h, PAAD cells (1.5 × 103 per well) were harvested and cultured in a 96-well plate for an additional 5 days. Then, 10 μL of CCK-8 test reagent was added each day and the number of living cells was measured by absorbance at 460 nm after 2 h.
5
Cell Growth Kinetics Assay
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Two thousand to 3,000 tGBM cells per well were seeded on a 96-well plate. Cell growth at different time points was measured using the Cell Counting Kit 8 (Abcam, catalog # ab228554) following the product protocol. Absorbance at 460 nm was measured using a Synergy 2 multi-mode microplate reader (BioTek).
6
Cell Proliferation Assay Using WST-8
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Cell proliferation was performed by using Cell Counting Kit 8 (WST-8, Abcam) as per the manufacturer's instructions. Briefly, 0.03 × 106 cells were seeded on tissue-culture clear bottom microplates (Corning) in their corresponding media (100 μL). When indicated, cells were treated with the indicated compounds and for the indicated time points. 10 μL/well of WST-8 solution was added and incubated for 2 hours at 37°C before measuring absorbance at 460 nm. For each experiment, the absorbance of the blank wells (growth media and vehicle/treatment) was subtracted from the values for those wells with cells.
7
Cell Viability and Colony Formation
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Cells were transfected with the indicated oligonucleotide or plasmid as described above, followed by the incubation for 24 h at 37°C. The Cell Counting Kit-8 (CCK-8), a sensitive colorimetric assay, was used to determine cell viability as per the suggestion of manufacturers (Abcam, Cambridge, UK). For colony formation experiments, the transfected cells were incubated for 15 days at 37°C under 5% CO2, and then the colonies (at least 50 cells) were scored after being visualized by staining with 1% crystal violet.
8
Magnolol Cytotoxicity Evaluation
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Approximately 2,000 cells were inoculated in each well of 96-well plates and incubated with a concentration range of Magnolol. A Cell Counting Kit-8 (ab228554, abcam, shanghai China) was applied to test the cellular viability. The experiments were performed five times independently.
9
Cell Viability Assay with WST-8
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Cell Counting Kit 8 (WST-8) (Abcam, Shanghai, China) was purchased to test the cell viability for target cells. We have cultured target cells for 24, 48 and 72 hours respectively for the cell viability test. After culture, 100 μL 1×105 cells were added to each well and 10 μL WST solution was added to each well and incubated for 4 hours at 37 °C. Then OD results were measured at 460 nm.
10
Cell Viability Assay using CCK-8
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Cell viability was assessed using Cell Counting Kit 8 (Abcam, Cambridge, UK). The method is based on the reduction of tetrazolium salt to an orange formazan, which can be detected colorimetrically. The amount of produced formazan is proportional to the number of cells alive. Briefly, cells were seeded in a 384-well plate with a density of 500 cells/well/100 µL and treated 24 h afterwards. After 6 days, 5 µL of the premixed water-soluble tetrazolium salt solution was added into each well. After incubation for 4 h, the absorbance was measured at 460 nm using a microplate reader (Tecan infiinite M200, Zurich, Switzerland).
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