Hydrochloric acid (hcl)

Manufactured by Solarbio

Sourced in China

HCl is a chemical compound consisting of one hydrogen atom and one chlorine atom. It is a colorless, corrosive, and highly reactive gas that is commonly used in various scientific and industrial applications.

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Lab products found in correlation

7 protocols using hydrochloric acid (hcl)

Fucoidan Extraction and Characterization

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Fucoidan standard extracted from U. pinnatifida (Fstd) was purchased from Sigma-Aldrich (Buchs, Switzerland). Sugar standards including _L-fucose, _D-galactose, and _D-glucose were purchased from Macklin (Shanghai, China). Synthetic antioxidants BHA, sodium hydroxide (≥99.0%), and ascorbic acid were also purchased from Macklin (Shanghai, China). Anhydrous ethanol (≥99.0%), hydrochloric acid (4 M), trifluoroacetic acid (≥99.0%), ferrous sulfate (9 mM), hydrogen peroxide (0.3%), ethanolic solution of salicylic acid (5 mM), potassium ferricyanide, 1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-hydrazyl (DPPH) and dialysis bags were purchased from Solarbio (Beijing, China). All other chemicals used in this study were of analytical grade.

Yu J., Li Q., Wu J., Yang X., Yang S., Zhu W., Liu Y., Tang W., Nie S., Hassouna A., White W.L., Zhao Y, & Lu J. (2021). Fucoidan Extracted From Sporophyll of Undaria pinnatifida Grown in Weihai, China – Chemical Composition and Comparison of Antioxidant Activity of Different Molecular Weight Fractions. Frontiers in Nutrition, 8, 636930.

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Histopathological Analysis of Renal Cell Carcinoma

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The tissue specimens were fixed in 10% neutral formaldehyde for 16-18 h at room temperature, dehydrated through graded ethanol (50, 70, 80, 90 and 100%), transparentized in xylene at 35°C for 30 min and embedded in paraffin at 60°C for 30 min. Subsequently, the specimens were sliced into serial sections at a thickness of 5 µm and allowed to stand at 45°C. Next, the sections were extracted, baked at 60°C for 1 h, deparaffinized by washing with xylene and rehydrated. Then, H&E staining was performed at a temperature of 35°C as follows: the sections were stained by hematoxylin for 10 min, washed with water for 15 min, differentiated with 1% hydrochloric acid-ethanol for 3 sec, washed with water for 10 sec and stained with 0.5% eosin for 3 min (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China). Then, the sections were dehydrated with graded ethanol, transparentized with xylene and sealed with neutral gum. The pathological changes of the RCC tissues and adjacent normal tissues were observed under an optical microscope (magnification, ×200) (CX31-LV320; Olympus Corporation, Tokyo, Japan).

Hong X, & Yu J.J. (2019). Silencing of lysyl oxidase-like 2 inhibits the migration, invasion and epithelial-to-mesenchymal transition of renal cell carcinoma cells through the Src/FAK signaling pathway. International Journal of Oncology, 54(5), 1676-1690.

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Bioactive Compound Extraction from Hemerocallis citrina

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Hemerocallis citrina Borani was obtained from Qingyang, Gansu, China. Standards of glucose, mannose, fucose, rhamnose, xylose, galactose, arabinose, glucuronic acid, ribose, and galacturonic acid were purchased from Shanghai Yuanye Biotechnology (Shanghai, China). Absolute ethanol was purchased from Fuyu Fine Chemical (Tianjin, China). Trifluoroacetic acid, methanol, sodium hydroxide, hydrochloric acid, carbazole, 1-phenyl-3-methyl-5-pyrazolone, 3,5-dinitrosalicylic acid, sulfuric acid, Coomash bright blue, chloroform, potassium bromide, sodium azide, sodium acetate, anthranone, and other reagents were purchased from Solarbio Biotechnology (Beijing, China). ABTS and FRAP assay kits were purchased from Suzhou Keming Biotechnology (Suzhou, China). Distilled water was used in these experiments.

Ti Y., Zhang Y., Ban Y., Wang X., Hou Y, & Song Z. (2022). Polysaccharide from Hemerocallis citrina Borani by subcritical water with different temperatures and investigation of its physicochemical properties and antioxidant activity. Frontiers in Nutrition, 9, 982695.

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Tilapia Protein Extraction Protocol

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Fresh tilapia (500 g ± 100 g) was purchased from the Yonghui supermarket (a local supermarket in Fuzhou, China). Curdlan was purchased from VWR Life Science Amresco Products, Avantor Performance Materials, Inc. Tris, HCl, KCl, NaN₃, β‐mercapto ethanol, Mg (CH₃COO)₂, ethylene glycol‐bis‐(2‐chloroethyl) tetraacetic acid (EGTA), ATPNa₂, KHCO₃, MgCl₂, formaldehyde, glutaraldehyde, phosphoric acid salt buffer, ethanol, and acetone were obtained from Solarbio Science & Technology Co., Ltd. The above reagents were all of analytical grade.

Li Q., Wang P., Miao S., Zhang L, & Zheng B. (2019). Curdlan enhances the structure of myosin gel model. Food Science & Nutrition, 7(6), 2123-2130.

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Biomimetic Hydroxyapatite-Based Composites

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PVA (DS = 0.61 and number-average polymerization degree of 1799, 98~99%), hydroxyapatite (HA) > 12 nm particle size and α-d-lactose were analytical grade and received from Aladdin Chemistry Co. (Shanghai, China). Tris (hydroxymethyl) amino-methane (Tris) (99.9%) and HCl were analytical grade reagents purchased from Solarbio (Beijing, China) and used as supplied. Glutaraldehyde was obtained from Aladdin Chemistry Co. (Shanghai, China). Deionized water was used in all experiments.

Wang Y., Xue Y., Wang J., Zhu Y., Zhu Y., Zhang X., Liao J., Li X., Wu X., Qin Y.X, & Chen W. (2019). A Composite Hydrogel with High Mechanical Strength, Fluorescence, and Degradable Behavior for Bone Tissue Engineering. Polymers, 11(7), 1112.

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Isolating Trichinella spiralis Larvae

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The T. spiralis isolate ISS534 was maintained by serial passaging in ICR mice in the laboratory of animal care in the center of Jilin Agricultural University (Jilin, China). Individual T. spiralis larvae were isolated from mice infected for more than 35 dpi by digesting mouse carcasses with 1% HCl and 1% pepsin (1:3,000; Solarbio, Beijing, China; Li et al., 2010 (link)). Experimental mice were orally administered 350 muscle larvae. The 8-week-old male BALB/c mice were obtained from Hua Fu Kang Biotechnology (Beijing, China) and allowed to eat and drink freely until the end of the experiment. The use of mice in this study was approved by the Animal Ethics Committee and Institutional Life Science Ethics Committee of Jilin Agriculture University (permit no. JLAU 20200904001). The experiments were carried out in strict accordance with the National Laboratory Animal Welfare Guidelines. If any animal has labored breathing, bleeding diarrhea, or becomes moribund it will be euthanized immediately by CO₂ inhalation.

Xue Y., Zhang B., Wang N., Huang H.B., Quan Y., Lu H.N., Zhu Z.Y., Li J.Y., Pan T.X., Tang Y., Jiang Y.L., Shi C.W., Yang G.L, & Wang C.F. (2022). Oral Vaccination of Mice With Trichinella spiralis Putative Serine Protease and Murine Interleukin-4 DNA Delivered by Invasive Lactiplantibacillus plantarum Elicits Protective Immunity. Frontiers in Microbiology, 13, 859243.

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Purification of Periplocoside E from P. sepium

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Periplocoside E was extracted and purified from P. sepium Bunge (root barks) in the Institute of Pesticide Science, Northwest A & F University (NWAFU, Yangling, China) [37 ]. The chemical structure of PS-E is shown in Figure 1. PS-E has a purity of over 98% based on high performance liquid chromatography (HPLC) analysis. EAH sepharose 4B was purchased from general electronics (GE) Healthcare (Beijing, China). Tris, NaCl, HCl, acetic acid, sodium acetate, and all other chemicals were purchased from Solarbio (Beijing, China).

Feng M., He Z., Wang Y., Yan X., Zhang J., Hu Z, & Wu W. (2016). Isolation of the Binding Protein of Periplocoside E from BBMVs in Midgut of the Oriental Amyworm Mythimna separata Walker (Lepidoptera: Noctuidae) through Affinity Chromatography. Toxins, 8(5), 139.

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