U-87 MG cells were suspended in PBS (50 μL, 2 × 106 cells), mixed with 25 μL of Matrigel (BASEMENT MEMBRANE MATRIX, BD Medical Technology, USA), and injected subcutaneously into nude mice on the right flank. When the tumor volume reached 100 mm3 (usually within 10 days), PSP NPs or PPSP NPs containing Cy5-siRNA were intravenously (i.v.) injected (1 mg siRNA/kg, 200 μL). At 1 and 4 h post injection, animals were anesthetized with 3% isoflurane and imaged by a Maestro In Vivo Imaging System (PerkinElmer, MA, USA) using green excitation filter (572–621 nm) with the exposure time of 100 ms. Mice were then euthanized, and the tumors as well as major organs (heart, liver, spleen, lung, and kidney) were harvested before being imaged using the Maestro In Vivo Imaging System as well. The tumor tissues were further frozen in the OCT embedding medium (Sakura, CA, USA). Frozen sections of 20-μm thickness were prepared with a cryotome Cryostat (CM 1900, Leica, Germany), and were stained with Hoechst (300 nM) for 15min at room temperature. After washing with PBS for twice, the frozen sections were mounted with the Prolong Gold® (Invitrogen) mounting medium and observed under CLSM (Model 700, Zeiss, Germany).
Maestro in vivo imaging system
Manufactured by PerkinElmer
Sourced in United States
The Maestro In Vivo Imaging System is a laboratory equipment designed for non-invasive, real-time imaging of small animals. It utilizes advanced optical imaging technology to capture and analyze fluorescent and bioluminescent signals from biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Cryotome cryostat, by Leica camera (1 mentions)
Prolong gold, by Thermo Fisher Scientific (1 mentions)
Matrigel, by BD (1 mentions)
μ slide 8 well, by Ibidi (1 mentions)
Dm4000b microscope, by Leica camera (1 mentions)
Molecular imaging software, by Carestream (1 mentions)
3d bioplotter, by EnvisionTEC (1 mentions)
22 protocols using maestro in vivo imaging system
1
In Vivo Tracking of Cy5-siRNA Nanoparticles
2
In Vivo Tumor Imaging with DiR-Loaded Liposomes
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When the tumor volume was about 50–100 mm3, the female BALB/C nude mice were randomized into three groups (n=3 for each group) and treated with free DiR, DiR-loaded LDP2000, LPP2000, or LPP5000 at a dose of DiR (5 mg/kg) via the tail vein. At 24 hours, the mice were administered chloral hydrate intraperitoneally. Image acquisition was performed on a Maestro In Vivo Imaging System (PerkinElmer Inc., Waltham, MA, USA).
3
Xenograft Formation and Intracranial Injection
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All animal procedures were approved by the Animal Care and Ethical Committee of College of Life Sciences at Wuhan University. Xenografting experiments were carried out using 6-week-old male Balb/c athymic nude mice (HNSJA, Changsha, China). Mice were housed in a certified specific-pathogen-free (SPF) facility. U251 glioma cells were transfected with lentivirus (moi≈10) expressing scramble control or HMGA2 shRNAs 48 hours before inoculation. A total of 1×106 suspended cells (in 1×PBS) were inoculated subcutaneously in each side of the anterior lateral thoracic wall. Tumor dimensions were measured every week and animals were sacrificed 4 weeks after inoculation. A total of 1×105 lentivirus-transduced GICs (in culture medium) were injected intracranially using a stereotactic device (RWD) and a Hamilton syringe at a depth of 2.5 mm into the right striata of cerebral hemisphere [55 (link)]. Animals were 8 weeks post-surgery or when they showed significant signs of tumor formation (tremor, hunching or seizure). Xenografted nude mice were transcardiac perfused with 4% PFA and brains were dissected out. Fluorescent images of brains were captured using the Maestro In-vivo Imaging System (PerkinElmer, Waltham, MA, USA).
4
Photothermal Therapy of PSMA-Positive Tumors
5
Longitudinal Tumor Imaging of Cy5-Met Peptide
6
In Vivo T Cell Trafficking Monitoring
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To monitor cell trafficking, human T cells were stained with Q Tracker 800 Cell Labeling Kit (Life Technologies) according to the manufacturer’s instructions. Cells were injected into the tail vein of NSG mice and images were acquired after 18 h (Maestro in vivo imaging system, Perkin Elmer).
7
Multicolor Fluorescence Imaging Versatility
8
In Vivo Fluorescence Imaging of Tumors
9
Fluorescence Imaging of Glycosidase Activity
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A
50 μM solution of glycosidase-reactive fluorescent probe
(200 μL) in PBS containing 0.5% v/v DMSO as a cosolvent was
added to each well of an 8-well chamber (μ-Slide 8 well; Ibidi)
containing a human surgical specimen (tumor or nontumor tissue). The
fluorescence intensities and images of specimens were recorded with
the Maestro in vivo imaging system (PerkinElmer)
before and at 1, 3, 5, 10, 20, and 30 min after probe administration.
The green filter setting (Ex/Em = 490 nm/550 nm long-pass) was used.
The tunable filter was automatically increased in 10 nm increments
from 500 to 720 nm, while the camera sequentially captured images
at each wavelength interval. Fluorescence at 540 nm was extracted,
and fluorescence intensities were quantified by drawing ROIs with
the Maestro software. Exposure time was set at 100–50 ms depending
on fluorescence intensity from specimens. The stage and lamp of the
equipment were both set at position 1. Surgical specimens were frozen
immediately after resection, stored at −80 °C, and thawed
at room temperature before use in this experiment.
50 μM solution of glycosidase-reactive fluorescent probe
(200 μL) in PBS containing 0.5% v/v DMSO as a cosolvent was
added to each well of an 8-well chamber (μ-Slide 8 well; Ibidi)
containing a human surgical specimen (tumor or nontumor tissue). The
fluorescence intensities and images of specimens were recorded with
the Maestro in vivo imaging system (PerkinElmer)
before and at 1, 3, 5, 10, 20, and 30 min after probe administration.
The green filter setting (Ex/Em = 490 nm/550 nm long-pass) was used.
The tunable filter was automatically increased in 10 nm increments
from 500 to 720 nm, while the camera sequentially captured images
at each wavelength interval. Fluorescence at 540 nm was extracted,
and fluorescence intensities were quantified by drawing ROIs with
the Maestro software. Exposure time was set at 100–50 ms depending
on fluorescence intensity from specimens. The stage and lamp of the
equipment were both set at position 1. Surgical specimens were frozen
immediately after resection, stored at −80 °C, and thawed
at room temperature before use in this experiment.
10
In vivo Imaging of JAM-A Targeting
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Imaging was performed with the aid of the Maestro In Vivo Imaging System (PerkinElmer, Waltham, MA) with each mouse receiving 20-μg of JAM-A mAb/IR700. For a probe competition, in vivo assay, JAM-A mAb/IR700 conjugates (20-μg) and JAM-A mAb (40-μg) were mixed immediately before i.v. injections. Norm Ab/IR700 conjugates (20-μg) or IR700 alone (5.0-mg/mL) were used as controls. All compounds were mixed with sterile 1X PBS followed by tail vein injection. Imaging was performed at different time points using deep-red filter set. During imaging, the temperature of imaging bed was kept at 37 °C. Mice received inhalation of isoflurane through a nose cone attached to the imaging bed. Mice were imaged over 2-day post-injection, after which, the mice were sacrificed and tissues such as liver, kidneys, spleen, heart, lung, seminal vesicles along with prostate gland, and tumor xenografts were harvested for ex vivo imaging. Quantification of fluorescent signals was obtained by calibration of IR700 using the 780-nm channel.
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