Dlx5 6 cre

Manufactured by Jackson ImmunoResearch

The Dlx5/6-Cre is a Cre recombinase expressed under the control of the Dlx5/6 promoter. It is used to drive Cre-mediated recombination in cells expressing the Dlx5 and Dlx6 genes.

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Lab products found in correlation

B6 cg tg actflpe 9205dym j, by Jackson ImmunoResearch (1 mentions) C57bl 6j mice, by Jackson ImmunoResearch (1 mentions) Nestin cre, by Jackson ImmunoResearch (1 mentions)

2 protocols using dlx5 6 cre

Conditional Knockout Mice for CDKL5 Study

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The Cdkl5 floxed mouse line with Cre-dependent excision of exon 7 (based on revised Cdkl5 gene nomenclature; formerly exon 6) was used for the generation of conditional knockout mice^{14 (link)}. Dlx5/6-Cre, a mouse line expressing Cre in forebrain inhibitory neurons^{17 (link)}, was obtained from Jackson Laboratories and maintained in the C59BL/6J background (Stock No. 008199). The CDKL5 R59X knock-in line was generated as follows: a targeting vector was designed to insert a frt-flanked neomycin resistance (neo) cassette downstream of exon 5, and a single-nucleotide change of C to T, leading to a nonsense mutation at arginine 59 (R59X) of the Cdkl5 gene. The construct was electroporated into C57BL/6N embryonic stem (ES) cells. Correctly targeted ES cells were injected into BALB/c blastocysts and resulting chimeric mice were bred with B6.Cg-Tg(ACTFLPe)9205Dym/J (Jackson Laboratories, Stock No. 005703) to remove the neo cassette. Resulting offspring were bred to C57BL/6J mice (Jackson Laboratories, Stock No. 000664) for at least ten generations. The R59X knock-in mouse line has been deposited at Jackson Laboratories (Stock No. 028856).

Tang S., Terzic B., Wang I.T., Sarmiento N., Sizov K., Cui Y., Takano H., Marsh E.D., Zhou Z, & Coulter D.A. (2019). Altered NMDAR signaling underlies autistic-like features in mouse models of CDKL5 deficiency disorder. Nature Communications, 10, 2655.

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Generation and Characterization of Tor1 Floxed Mice

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Animal testing was conducted in accord with the National Institutes of Health laboratory animal care guidelines and with the Institutional Animal Care and Use Committee at the University of Michigan. Prenatal birthdating of mice was determined by the presence of a vaginal plug, designated as embryonic day 0.5 (E 0.5). For postnatal birthdating, the day of birth was designated as P0. Nestin-Cre, Synapsin 1-Cre, and Dlx5/6-Cre mice were obtained from The Jackson Laboratory. Tor1a floxed mice were described previously (Liang et al., 2014 (link)). Tor1b floxed mice were generated by introducing LoxP sites in the Tor1b introns in between exons 2 and 3 and after exon 5 (Fig. S3). Tor1(ab) floxed animals contain LoxP sites in the Tor1a intron between exons 2 and 3, and the Tor1b intron between exons 2 and 3 (Fig. 3). Both Tor1b and Tor1(ab) floxed mice are generated by the standard method, as described previously (Liang et al., 2014 (link)). Mouse genotyping and breeding is described in detail in Supplemental Experimental Procedures.

Tanabe L.M., Liang C.C, & Dauer W.T. (2016). Neuronal Nuclear Membrane Budding Occurs During a Developmental Window Modulated by Torsin Paralogs. Cell reports, 16(12), 3322-3333.

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