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Anti eif4g

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Anti-eIF4G is an antibody that binds to the eIF4G protein, which is a component of the eukaryotic translation initiation complex. The eIF4G protein plays a crucial role in the recruitment of the ribosome to the mRNA during the initiation of protein synthesis.

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Lab products found in correlation

Anti eif4e, by Cell Signaling Technology (8 mentions) Anti akt, by Cell Signaling Technology (4 mentions) Anti 4e bp1, by Cell Signaling Technology (4 mentions) Anti β actin, by Cell Signaling Technology (2 mentions) Anti eif4ai, by Cell Signaling Technology (2 mentions) Anti phospho eif2α ser51 d9g8, by Cell Signaling Technology (2 mentions) Anti phospho 4ebp thr37 46 236b4, by Cell Signaling Technology (2 mentions) Anti β actin ab20272, by Abcam (2 mentions) Anti sbp tag sb19 c4 sc 101595, by Santa Cruz Biotechnology (2 mentions) Pierce ecl western blotting substrate, by Thermo Fisher Scientific (2 mentions) Ab205016, by Abcam (1 mentions) Ab22553, by Abcam (1 mentions) Ab60126, by Abcam (1 mentions) Ab151230, by Abcam (1 mentions) Ab181602, by Abcam (1 mentions) Rabbit anti parp1, by Cell Signaling Technology (1 mentions) Rabbit anti hspa8, by Cell Signaling Technology (1 mentions) A5441, by Merck Group (1 mentions) F1804, by Merck Group (1 mentions) Anti her2, by Cell Signaling Technology (1 mentions)

11 protocols using anti eif4g

1

Antibody Analysis in Immunoblotting and Immunostaining

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Antibodies used for western blot analysis or immunostaining were as follows: mouse anti-FLAG M2 (F1804, Sigma), rabbit anti-PCIF1 (ab205016, Abcam), mouse anti-β actin (A5441, Sigma), anti-eIF4E (2067, Cell Signaling), anti-eIF4G (2498, Cell Signaling), rabbit anti-GAPDH (ab181602, Abcam), mouse anti-TRIM28 (ab22553, Abcam), rabbit anti-ATF5 (ab60126, Abcam), rabbit anti-EEF2 (ab33523), mouse anti-RACK1 (B-3, Santa Cruz), rabbit anti-PARP1 (9542, Cell Signaling), rabbit anti-HSPA8 (8444, Cell Signaling), mouse anti-HSP70/72 (C92F3A-5, Enzo Life Sciences). For m6A individual-nucleotide-resolution cross-linking and immunoprecipitation (miCLIP), rabbit anti-m6A (ab151230, Abcam) was used.
Boulias K., Toczydłowska-Socha D., Hawley B.R., Liberman N., Takashima K., Zaccara S., Guez T., Vasseur J.J., Debart F., Aravind L., Jaffrey S.R, & Greer E.L. (2019). Identification of the m6Am methyltransferase PCIF1 reveals the location and functions of m6Am in the transcriptome. Molecular cell, 75(3), 631-643.e8.
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2

Immunoblotting of Signaling Proteins

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The following anti-human antibodies were used for immunoblotting: anti-phosphorylated Akt at Ser-473 (#4060, Cell Signaling Technology), anti-Akt (#4685, Cell Signaling Technology), anti-phosphorylated Erk1/2 at Thr202/Thy204 (#9106, Cell Signaling Technology), anti-Erk1/2 (#9102, Cell Signaling Technology), anti-phosphorylated 4EBP1 at Ser-65 (#9451, Cell Signaling Technology), anti-ER (#13258, Cell Signaling Technology), anti-4EBP1 (#9452, Cell Signaling Technology), anti-phosphorylated HER2 at Tyr-1221/1222 (#2243, Cell Signaling Technology), anti-HER2 (#2248, Cell Signaling Technology), anti-eIF4G (#2498, Cell Signaling Technology), anti-eIF4E (#9742, Cell Signaling Technology), anti-eIF4A (#2013, Cell Signaling Technology), and anti-β-actin (#4970, Cell Signaling Technology).
Fujimoto Y., Morita T.Y., Ohashi A., Haeno H., Hakozaki Y., Fujii M., Kashima Y., Kobayashi S.S, & Mukohara T. (2020). Combination treatment with a PI3K/Akt/mTOR pathway inhibitor overcomes resistance to anti-HER2 therapy in PIK3CA-mutant HER2-positive breast cancer cells. Scientific Reports, 10, 21762.
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3

Molecular Tools for Neuroscience Research

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Antibodies, plasmids and other reagents were obtained from the following resources. Antibodies: anti-GluA1Ct (rabbit) was made by OriGene; anti-GluA2/3 (rabbit), anti-NR1Ct (mouse), anti-PSD95 (mouse), anti-eIF4e (rabbit) were from Millipore; anti-αTubulin (mouse) was from Sigma-Aldrich; anti-phosphoAMPKαThr172 (rabbit), anti-Phospho-AktSer473 (rabbit), anti-eIF4g (rabbit) and anti-Akt (rabbit) were from Cell Signaling; anti-SIRT1 (rabbit) was from Abcam. Chemicals: Resveratrol, MG132, Leupeptin, Cycloheximide, Anisomycin, Actinomycin D, Compound C, LY 294002, STO609, Dimethyl sulfoxide (DMSO) were from Sigma-Aldrich; EX527 and 4EGI-1 were from Millipore; 5-aminoimidazole-4-carboxamide-1-β-D-riboside (AICAR) was from Enzo Life Sciences; Plasmids and siRNAs: pcDNA was purchased from Invitrogen; EGFP was purchased from Clontech Laboratories. αAMPK kinase dead (AMPK K.D.) and PI3 kinase dominant negative form (PI3K D.N.) were generously provided by Prof. Lewis C. Cantley (Harvard Medical School, Boston, MA); G-CaMP3 was generously provided by Prof. Loren L. Looger (Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, VA) via his deposit at Addgene (#22692); siRNAs (Scramble and SIRT1) were purchased from Santa Cruz Biotechnology.
Wang G., Amato S., Gilbert J, & Man H.Y. (2015). Resveratrol Up-regulates AMPA Receptor Expression via AMP-activated protein kinase – mediated Protein Translation. Neuropharmacology, 95, 144-153.
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4

Protein Expression Analysis by Western Blot

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Experimental procedures used have been previously described (17 (link)). Lysates were clarified, then protein concentration was determined using the bicinchonic acid protein assay reagent kit (Pierce). Even amounts of protein (40 or 50 μg) were resolved on an SDS-PAGE gel and transferred to a nitrocellulose membrane. Blots were probed with the following primary antibodies: anti-actin (Sigma); anti-eIF4E (Transduction Laboratories); anti-eIF4E (Cell Signaling); anti-eIF4G from Dr. Sonenberg (McGill University). ImageJ software was utilized to determine relative density of protein bands.
Fagan D.H., Fettig L.M., Avdulov S., Beckwith H., Peterson M.S., Ho Y.Y., Wang F., Polunovsky V.A, & Yee D. (2017). Acquired Tamoxifen Resistance in MCF-7 Breast Cancer Cells Requires Hyperactivation of eIF4F-Mediated Translation. Hormones & Cancer, 8(4), 219-229.
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5

Eukaryotic Translation Initiation Complex Analysis

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Anti-eIF4AI (#2490, Cell signaling) (1:1000), anti-phospho-eIF2α (Ser51) (D9G8 #3398, Cell Signaling) (1:1000), anti-4E-BP1 (#9452, Cell Signaling) (1:2000), anti-phospho-4EBP (Thr37/46) (236B4 #2855, Cell Signaling) (1:2000), anti-β-actin (ab20272, Abcam) (1:1000), anti-eIF4E (#9742, Cell Signaling) (1:1000), anti-eIF4G (#2498, Cell Signaling) (1:1000), and anti-SBP-tag (SB19-C4 sc-101595, Santa Cruz Biotechnology) (1:1000) were used as primary antibodies. Chemiluminescence was induced by Pierce ECL Western Blotting Substrate (Thermo Scientific) and images were acquired by FluorChem R imaging sysmtem (ProteinSimple).
Iwasaki S., Floor S.N, & Ingolia N.T. (2016). Rocaglates convert DEAD-box protein eIF4A into a sequence-selective translational repressor. Nature, 534(7608), 558-561.
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6

Eukaryotic Translation Initiation Complex Analysis

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Anti-eIF4AI (#2490, Cell signaling) (1:1000), anti-phospho-eIF2α (Ser51) (D9G8 #3398, Cell Signaling) (1:1000), anti-4E-BP1 (#9452, Cell Signaling) (1:2000), anti-phospho-4EBP (Thr37/46) (236B4 #2855, Cell Signaling) (1:2000), anti-β-actin (ab20272, Abcam) (1:1000), anti-eIF4E (#9742, Cell Signaling) (1:1000), anti-eIF4G (#2498, Cell Signaling) (1:1000), and anti-SBP-tag (SB19-C4 sc-101595, Santa Cruz Biotechnology) (1:1000) were used as primary antibodies. Chemiluminescence was induced by Pierce ECL Western Blotting Substrate (Thermo Scientific) and images were acquired by FluorChem R imaging sysmtem (ProteinSimple).
Iwasaki S., Floor S.N, & Ingolia N.T. (2016). Rocaglates convert DEAD-box protein eIF4A into a sequence-selective translational repressor. Nature, 534(7608), 558-561.
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7

Western Blotting Antibody Detection

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Western blotting was performed as previously described [37 (link)]. The primary antibodies used were as follows: anti-Myc-tag antibody (#2276; 1:1000), anti-peIF4E Ser-209 (#9741; 1:1000), anti-Mnk1 C4C1 (#2195; 1:1000), anti-eIF4G (#2498; 1:1000), anti-YB1 (#4202; 1:1000), anti-mTOR 7C10 (#2983; 1:1000), anti-β-catenin (#9562; 1:1000; all from Cell Signaling); anti-EIF4ENIF1 (three different antibodies: 1:500; Sigma; 1:1000 Cell Signaling; 1:1000 Abnova); anti-Cyclin D1 M-20 (1:1000; Santa Cruz Biotechnology); anti-MCL1 Y37 (1:500), anti-Ago2 (1:500), anti-GAPDH 6C5 (1:2000; all from Abcam); and anti-HuR (1:500; Millipore). Anti-actin CP01 (1:500; Calbiochem, Darmstadt, Germany) was used as a loading control. The secondary antibodies used were donkey anti-rabbit IgG-HRP (NA9340; 1:2000) and donkey anti-mouse IgG-HRP (NA9340; 1:2000; both from Amersham Pharma-Biotech, Uppsala, Sweden). Bound antibodies were visualized with an enhanced chemiluminescence detection kit (Amersham Pharma-Biotech).
Martínez A., Sesé M., Losa J.H., Robichaud N., Sonenberg N., Aasen T, & Ramón y Cajal S. (2015). Phosphorylation of eIF4E Confers Resistance to Cellular Stress and DNA-Damaging Agents through an Interaction with 4E-T: A Rationale for Novel Therapeutic Approaches. PLoS ONE, 10(4), e0123352.
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8

Antibody and Inhibitor Reagents for Signaling Studies

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The following antibodies were purchased from Cell Signaling Technology: Anti-Pim-1 (Cat#3247), anti-Pim2 (Cat#4730), anti-Pim3 (Cat#4165) anti-MET (Cat#8198), anti-phospho-MET (Cat#3077), anti-BAD (Cat#9239), anti-phospho-BAD (Cat#5284), anti-eIF4B (Cat#3592), anti-phospho-eIF4B (S406, Cat#8151), anti-eIF4G (Cat#2498), anti-eIF4E(Cat#2067), anti-Myc-Tag(Cat#2272), anti-AKT (pan, Cat#4691), anti-phospho-AKT (S473, Cat#4058), anti-phospho-4E-BP1 (Cat#2855), anti-4E-BP1 (Cat#9452), anti-phospho-S6 (Cat#2215), anti-ERK (Cat#9102), anti-phospho-ERK (Cat#9101), anti-Bcl-2(Cat#4223), anti-Bim(Cat#2933), and anti-cleaved PARP(Cat#5625). Anti-β-actin (Cat#A3854), anti-β-tubulin (Cat#T4026), anti-FLAG (Cat#F1804) antibodies were purchased from Sigma. Anti-Mcl-1 antibody (Cat#sc-12756) was from Santa Cruz Biotechnology. A neutralizing antibody against MET was from Abcam (Cat#ab10728). HRP-linked enhanced chemiluminescence (ECL) mouse (Cat#NA931V) and rabbit IgG (Cat#NAV934V) were purchased from GE Healthcare Life Sciences.
The small molecule inhibitors PP242, BEZ235, ABT199, and ABT737 were obtained from Selleck Biochemicals. PHA665752 was from Cayman Chemical. LGB321 was provided by Norvatis. AZD6094 and AZD1208 were provided by AstraZeneca. LY2801653 was from Eli Lilly.
An N., Xiong Y., LaRue A.C., Kraft A.S, & Cen B. (2015). Activation of Pim Kinases Is Sufficient to Promote Resistance to MET Small Molecule Inhibitors. Cancer research, 75(24), 5318-5328.
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9

Antibody Detection and Pharmacological Inhibitors

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Antibodies against RPLp0, RPLp1, and RPLp2 were from Abcam (Cambridge, MA, USA). Antibodies anti-FLAG, anti-GFP, anti-eIF4E, anti-p-eIF4E, anti-eIF4G, anti-eEF1A, anti-GAPDH, and anti-β-actin were from Cell Signaling Technology (Danvers, MA, USA). Mouse monoclonal antibody 12D10 against puromycin was from Merck Millipore. Mouse polyclonal antibody to PEDV-N was from Alpha Diagnostic International (San Antonio, TX, USA). The pharmacological inhibitors CGP57380, cycloheximide (CHX), and MG132 were from MedChemExpress (Monmouth Junction, NJ, USA).
Dong H.J., Wang J., Zhang X.Z., Li C.C., Liu J.F, & Wang X.J. (2023). Proteomic screening identifies RPLp2 as a specific regulator for the translation of coronavirus. International Journal of Biological Macromolecules, 230, 123191.
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10

Characterization of Insulin Signaling Cascade

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The following commercial antibodies were employed: mouse monoclonal antibodies anti-PTBP1, anti-V5 (Invitrogen), anti-m3G/m7G-cap (Synaptic System), anti-γ-tubulin and anti-insulin (for western blotting) (Sigma), rabbit polyclonal antibodies anti-eIF4E, anti-ph-eIF4E (Ser-209), anti-4E-BP, anti-eIF4G, anti-AKT, anti-ph-AKT (Ser-473), anti-p70S6K, anti-ph-p70S6K (Thr-389) and anti-PABP (Cell Signaling Technology), anti-CgA (Abcam), anti-PC1/3 and anti-PC2 (GeneTex), guinea pig antibody anti-insulin (for immunocytochemistry) (Abcam), goat anti-mouse, anti-rabbit and anti-guinea pig IgGs conjugated with Alexa 488 or Alexa 568 (Molecular Probes), goat anti-mouse and anti-rabbit IgGs conjugated to horseradish peroxidase (Bio-Rad). The mouse anti-ICA512 mAb has been previously described in Ref. [25] . The following reagents were from commercial sources: Rapamycin and LY294002 (Cell Signaling Technology), the eIF4E/eIF4G interaction inhibitor (Calbiochem), m7GpppG cap-analog (New England Biolabs), m7GTP-Sepharose (GE Healthcare) and Dynabeads M270 streptavidin (Invitrogen), reagents for luciferase assay (Promega), 35S-methionine (Hartmann-Analytics), pro-/insulin ELISA (Mercodia) and insulin RIA (Millipore).
Knoch K.P., Nath-Sain S., Petzold A., Schneider H., Beck M., Wegbrod C., Sönmez A., Münster C., Friedrich A., Roivainen M, & Solimena M. (2014). PTBP1 is required for glucose-stimulated cap-independent translation of insulin granule proteins and Coxsackieviruses in beta cells. Molecular Metabolism, 3(5), 518-530.
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