Bi 847325

Manufactured by Boehringer Ingelheim

BI-847325 is a laboratory equipment product manufactured by Boehringer Ingelheim. It is designed to perform specific functions in a research or laboratory setting. The core function of BI-847325 is to [insert concise, factual description of the equipment's core function, without interpretation or extrapolation].

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Lab products found in correlation

Live dead viability stain, by Thermo Fisher Scientific (1 mentions) Trametinib, by Selleck Chemicals (1 mentions) Vx 680, by Selleck Chemicals (1 mentions)

4 protocols using bi 847325

Assessing Drug Effects on 3D Melanoma Spheroids

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3D melanoma spheroids were prepared using the liquid overlay method as described in (23 (link)) before being treated with vehicle (dimethyl sulfoxide) or 300nM – 3µM of BI-847325 (Boehringer Ingelheim) for 48h. Spheroids were washed, stained with Live/Dead viability stain (Invitrogen/ Life Technologies Corp.) and analyzed as described in (14 ). The percentage of dead cells was determined using ImageJ software.

Phadke M.S., Sini P, & Smalley K.S. (2015). The novel ATP-competitive MEK/Aurora kinase inhibitor BI-847325 overcomes acquired BRAF inhibitor resistance through suppression of Mcl-1 and MEK expression. Molecular cancer therapeutics, 14(6), 1354-1364.

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Evaluating BI-847325 Cytotoxicity

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Cells were plated at a density of 2.5 × 10³ cells per 100 µl and left to grow overnight before being treated with increasing concentrations of BI-847325 (Boehringer Ingelheim) for 72h. The metabolic activity was determined using Alamar blue reagent as per the manufacturer’s protocol.

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Apoptosis Induction by Kinase Inhibitors

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Cells were plated into 6 well plates at a density of 1 × 10⁵ cells per ml and left to adhere overnight. Cells were treated with 10nM, 100nM and 1µM BI-847325 (Boehringer Ingelheim), 30nM trametinib (Selleck), 1µM VX680 (Selleck) for 48h. Annexin V and TMRM staining was done as described in (21 (link)).

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Clonogenic Survival Assay of BI-847325

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Cells were grown overnight at a density of 1 × 10⁴ cells per ml and treated with vehicle (dimethyl sulfoxide) or with 30 nM, 100 nM, 300 nM and 1 µM of BI-847325 (Boehringer Ingelheim). The medium and drug/vehicle was replaced every two weeks. After 4 weeks of treatment the colonies were stained with crystal violet dye, as described in (22 (link)). The percent relative clonogenic survival was determined using ImageJ software.

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