2k x 2k ccd camera

Manufactured by Ametek

Sourced in United States

The 2k x 2k CCD camera is a high-resolution imaging device designed for scientific and industrial applications. It features a 2048 x 2048 pixel sensor that captures detailed images. The camera provides precise data collection and imaging capabilities.

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Lab products found in correlation

Carbon coated formvar support, by Ted Pella (1 mentions) Cm120 tem, by Philips (1 mentions) Tecnai t12 electron microscope, by Thermo Fisher Scientific (1 mentions) Digital micrograph software, by Ametek (1 mentions)

4 protocols using 2k x 2k ccd camera

Microtubule Preparation for Electron Microscopy

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To prepare microtubules for electron microscopy, samples of wild-type (3-–4 µM) or β:T238A αβ-tubulin (2 µM) were prepared in 100 mM PIPES pH 6.9, 10% glycerol, 2 mM MgSO₄, 0.5 mM EGTA, 1 mM GTP and incubated at 30 ˚C for 1 hr. 5 µl of the assembly reactions were spotted onto freshly glow-discharged 400 mesh grids with a carbon coated formvar support (Ted Pella), incubated for 30 s, rinsed with water, and stained with 2% aqueous uranyl acetate. Negatively stained grids were imaged at 23,000 x magnification using a Tecnai G2 Spirit electron microscope equipped with a 2Kx2K CCD camera (Gatan).

Geyer E.A., Burns A., Lalonde B.A., Ye X., Piedra F.A., Huffaker T.C, & Rice L.M. (2015). A mutation uncouples the tubulin conformational and GTPase cycles, revealing allosteric control of microtubule dynamics. eLife, 4, e10113.

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Transmission Electron Microscopy of PFF

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10 μL of the PFF (5 μg/μl sonicated and unsonicated PFF in PBS) samples were pipetted onto a 200 mesh copper grids (EMS) with carbon-coated formvar film and incubated for 2 min. Excess liquid was removed by blotting. The grid was briefly placed on 10 μL of 2% uranyl acetate (w/v; Merck, Darmstadt, Germany), followed by blotting to remove excess liquid. This last step was repeated. Grids were allowed to dry before imaging on a Phillips CM 120 TEM operating at 80 kV. Images were captured and digitized with an US1000 CCD (2048 x 2048 pixel, 14 μm pixel, 100% fill factor) and 2k x 2k CCD camera (Gatan, Inc. USA) by advanced microscopy techniques.

Kim H., Park J., Kang H., Yun S.P., Lee Y.S., Lee Y.I, & Lee Y. (2020). Activation of the Akt1-CREB pathway promotes RNF146 expression to inhibit PARP1-mediated neuronal death. Science signaling, 13(663), eaax7119.

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Cryo-EM Sample Preparation Protocol

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Carbon-coated grids were rendered hydrophilic prior to use by glow discharge (Fischione, Fishione Instruments, Export, PA, USA) in a plasma of 25% oxygen and 75% argon. The grids were then coated with 0.1 % poly-lysine to prevent capsid aggregation. Samples (~ 0.1 mg·mL⁻¹) were applied, and the grids were rinsed with distilled water and negatively stained with 1% uranyl acetate. Micro-graphs were recorded at 16 500× magnification on a Tecnai T12 electron microscope (FEI, Hillsboro, OR, USA) operating at 120 kV and equipped with a postcolumn energy filter (in zero-loss mode with a 20-eV energy slit width) and a 2kx2k CCD camera (Gatan, Pleasanton, CA, USA).

Watts N.R., Palmer I.W., Eren E., Steven A.C, & Wingfield P.T. (2019). Capsids of hepatitis B virus e antigen with authentic C termini are stabilized by electrostatic interactions. FEBS letters, 594(6), 1052-1061.

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Quantifying Glycogen Content in Stem Cells

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Specimens were analyzed in an FEI Tecnai^TM T12 transmission electron microscope (TEM). Images were captured using an Ultrascan 2k x 2k CCD camera and Gatan Digital Micrograph software. Data were collected using stereology principles to sample the stem cells as randomly as possible, and no assumptions were made about the orientation and distribution of the cells in the cross sections. Image contrast and magnifications were adjusted to visualize the ultrastructure of the glycogen bodies.
Planimetry analysis was performed using the ImageJ program (National Institutes of Health, Bethesda, MD) to draw contours around the regions of interest (ROI), which could be used to calculate the areas in square micrometers. Glycogen content in each cell was determined by computing the ratio between the cumulative areas of glycogen bodies and the total area of the cell section. A total of 50 cells were analyzed to provide sufficient statistical power in the analysis.

Chen R.J., Zhang G., Garfield S.H., Shi Y.J., Chen K.G., Robey P.G, & Leapman R.D. (2015). Variations in Glycogen Synthesis in Human Pluripotent Stem Cells with Altered Pluripotent States. PLoS ONE, 10(11), e0142554.

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