Linezolid

Compounds were purchased as powders from Sigma-Aldrich, except bedaquiline (Cellagen Technology), linezolid (Selleck Chemicals) and moxifloxacin (Fisher). Compounds were prepared in DMSO at 10 mM and stored in 70 μL aliquots at -20°C; stock solutions were thawed at RT overnight. Two-fold serial dilution series were prepared in 96-well polypropylene plates (Greiner) using a Biomek 3000 or 4000 (Beckman Coulter). Plates were sealed with DMSO-resistant thermal seals (Phenix) and stored at RT for up to 6 months.

Bacterial strains used in this study are presented in Tables 1 and 2. Mueller-Hinton broth (MHB), gentamicin and tetracycline were purchased from Sigma-Aldrich while mupirocin (Applichem), linezolid (Selleck Chemicals), and vancomycin hydrochloride (Gold Biotechnology) were acquired from other commercial vendors. Mannitol salt agar (MSA), Trypticase soy agar (TSA) and Trypticase soy broth (TSB) were purchased from Becton, Dickinson and Company (Cockeysville, MD). All statin drugs used in this study were purchased from Sigma-Aldrich (St. Louis, MO, USA), with the exception of pitavastatin and rosuvastatin which were obtained from Selleckchem (Houston, TX, USA).

Bacterial strains employed in this study are presented in Table 1. Mannitol salt agar (MSA) was purchased from Hardy Diagnostics (Santa Maria, CA). Muller-Hinton broth (MHB) was purchased from Sigma-Aldrich (St. Louis, MO). Trypticase soy broth (TSB) and Trypticase soy agar (TSA) were purchased from Becton, Dickinson (Cockeysville, MD). EB was purchased from (Adipogen corp, San Diego), vancomycin hydrochloride (Gold Biotechnology, St. Louis, MO), linezolid (Selleck Chemicals, Houston, TX), clindamycin (TCI chemicals, Portland, OR), erythromycin, rifampicin, ampicillin, gentamicin, chloramphenicol and fetal bovine serum (FBS) were purchased from Sigma-Aldrich (St. Louis, MO). DMEM media were purchased from Life technologies and MTS reagent (Promega, Madison, WI, USA).

Vancomycin-sensitive and Vancomycin-resistant Enterococcus faecalis strains and methicillin-resistant S. aureus (MRSA) strains included clinical isolates collected at the university hospital UZ Leuven (Belgium). The Vancomycin-sensitive S. aureus strain was ATCC 6538 (Rosenbach). The Vancomycine-intermediate S. aureus (VISA) HIP5827 (Tenover et al., 1998 (link)) and Vancomycin-resistant S. aureus (VRSA) were kindly donated by Olivier Denis (Université Libre de Bruxelles, Belgium). E. faecalis and S. aureus strains were grown at 37°C on brain heart infusion (BHI; Becton-Dickinson, Erembodegem, Belgium) agar and lysogeny broth (LB; also known as Luria-Bertani medium) agar plates, respectively. C. difficile strain ATJ ribotype 014/20 and strain AIU ribotype 027 were kept on brain heart infusion agar plates, grown at 37°C in an anaerobic cabinet. Vancomycin, daptomycin, tigeglycine, and linezolid were purchased from Selleck Chemicals (Munich, Germany).

The antimicrobials utilized in this work were cefazolin, gentamicin, erythromycin, tigecycline, rifampicin, daptomycin (purchased from Dalian Meilun Biotech, Dalin, China), ciprofloxacin, clindamycin, vancomycin (from the National Institutes for Food and Drug Control, Beijing, China), linezolid (Selleck Chemicals, Houston, TX, United States), and trimethoprim–sulfamethoxazole (Sigma–Aldrich, St Louis, MO, United States).