Special agents used in this study were as follows: recombinant human AR (Wako Chemicals USA, Inc., Richmond, VA), rabbit anti-human AR Ab (Santa Cruz Biotechnology Inc., Santa Cruz, CA), horseradish peroxidase-conjugated anti-phosphotyrosine mAb (clone RC20) (BD Transduction Laboratories, Fraklin Lakes, NJ), anti-phosphoserine mAb (Calbiochem-Novabiochem Corporation, San Diego, CA), rabbit anti-p44/42 MAP kinase Ab (Cell Signaling Technology, Inc., Danvars, MA), rabbit anti-phospho-p44/42 MAP kinase Ab (Thr202/Thr204) (Cell Signaling Technology, Inc.), rat anti-mouse CD3 mAb (Serotec Ltd., Oxford, UK), hamster anti-mouse CD28 mAb (Pharmingen Co., San Diego, CA), DynabeadsR mouse CD3/CD28 T cell expander (Invitrogen Dynal AS, Oslo, Norway), rat anti-mouse IL-2 mAb (R & D systems, Inc., minneapolis, MN), rat anti-mouse IFN-γ mAb (R & D systems), mouse IL-2 (R & D systems), mouse IFN-γ (R & D systems), D10.G4.1 T cell line (TIB 224; American Type Culture Collection (ATCC), Rockville, MD), epalrestat (Wako Pure Chemicals, Osaka, Japan), DL-glyceraldehyde (Wako), β-NADPH (Wako), imidazole (Wako), [3H]-thymidine (3H-TdR) (PerkinElmer Life Science Products Inc., Boston, MA).
Dl glyceraldehyde
Manufactured by Fujifilm
Sourced in Japan
DL-glyceraldehyde is a carbohydrate compound used in various laboratory applications. It serves as a versatile reagent and intermediate in biochemical and organic synthesis procedures. The compound has a molecular formula of C₃H₆O₃ and a systematic name of 2,3-dihydroxypropanal. DL-glyceraldehyde is a racemic mixture, containing both the D- and L-enantiomers of the aldehyde.
Automatically generated - may contain errors
Lab products found in correlation
Rat anti mouse ifn γ mab, by R&D Systems (1 mentions)
Mouse il 2, by R&D Systems (1 mentions)
β nadph, by Fujifilm (1 mentions)
Imidazole, by Fujifilm (1 mentions)
Mouse ifn γ, by R&D Systems (1 mentions)
Allyl alcohol, by Fujifilm (1 mentions)
N 2 3 dihydroxypropyl 13c5 valine, by Cambridge Isotopes (1 mentions)
3 chloro 1 2 propanediol 3 mcpd, by Merck Group (1 mentions)
L valine 13c5, by Cambridge Isotopes (1 mentions)
Propylene oxide, by Fujifilm (1 mentions)
D fructose, by Fujifilm (1 mentions)
Gelatin, by Fujifilm (1 mentions)
Ethanol, by Nacalai Tesque (1 mentions)
O phenylenediamine dihydrochloride, by Merck Group (1 mentions)
Sulphuric acid, by Fujifilm (1 mentions)
Nadph, by Oriental Yeast (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Tween 20, by Merck Group (1 mentions)
5 protocols using dl glyceraldehyde
1
Antibody-mediated Immune Cell Activation
2
Synthesis and Characterization of Food Contaminants
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Fluorescein isothiocyanate (FITC), glycidol, (±)-3-chloro-1,2-propanediol (3-MCPD) (purity 98.0%), and glycidyl oleate (purity 98.0%) were obtained from Sigma-Aldrich (St Louis, MO). Epichlorohydrin, propylene oxide, 1-bromopropane, allyl alcohol, D(-)-fructose, and DL-glyceraldehyde were obtained from FUJIFILM Wako Pure Chemical Industries Ltd. (Osaka, Japan). L-Valine-(13C5) (purity 98.0%) used for the synthesis of the internal standard N-(2,3-dihydroxypropyl)-(13C5)valine, were obtained from Cambridge Isotope Laboratories, Inc. (Tewksbury, MA, USA). All other chemicals and solvents used were analytical grade.
3
Inhibition of Aldose Reductase by Natural Compounds
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Dibasic sodium phosphate, sodium dihydrogen phosphate, D,L-glyceraldehyde, human recombinant aldose reductase (HR-AR), AG, quercetin, critric acid monohydrate, natrium carbonicum, sodium azide, gelatin and sulphuric acid were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Sodium bicarbonate, sodium chloride, potassium dihydrogen phosphate and ethanol were supplied by Nacalai Inc. (Kyoto, Japan). Tween 20, bovine serum albumin, glucose, O-phenylenediamine dihydrochloride, phosphate buffered saline, fetal bovine serum (FBS), steroyl myristoyl phosphatidylcholine glycine (SMPC Gly), 3-(4, 5-dimethylthazol-2-yl)-2, 5-diphenyl tetrazolium bromidetetrazolium salt (MTT) were purchased from Sigma-Aldrich company, Ltd. (St. Louis, MO, U.S.A.). NADPH was provided by Oriental Yeast Co., Ltd. (Tokyo, Japan). Anti-AGE antibody and goat anti-mouse IgG, HRP conjugate-secondary antibody Millipore (Merck U.S.A) were purchased from Transgenic Inc. (Hyogo, Japan). Silica gel 60 F254 TLC plates (Merck, U.S.A), silica gel 60 N (100–200 μm) and ODS were purchased from Kanto Chemical (Tokyo, Japan). DMSO was purchased from Wako Pure Chemical Industries, Ltd (Japan). Kaempferol-3-O-β-D -glucopyranoside was isolated from liquorice, and was identified by NMR and MS data [11 (link)].
4
Aldose Reductase Inhibition Activity Assessment
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All crude drugs and the Glycyrrhizae Radix preparata used in this study were purchased from Tochimoto Tenkaido Co., Ltd. (Osaka, Japan). Aldose reductase (EC1.1.1.21, 1-316aa, human, recombinant, E coli) was purchased from ATGen Co., Ltd. (Korea). D,L-glyceraldehyde and dimethyl sulfoxide (special grade) were purchased from Wako Pure Chemicals Industries, Ltd. (Osaka, Japan), and β-NADPH was purchased from Oriental Yeast Co. (Tokyo, Japan). Epalrestat was synthesized by Dr. Ryota Saito (Faculty of Science, Toho University).
5
Bullous Keratopathy Treatment Comparison
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According to the treatment protocols shown in Table 1 , these animals were randomly divided into five groups. Group A is the sham operated control group (only corneal incision, without descemetorhexis, n = 3). To establish the bullous keratopathy, the right eyes of animals in the other four groups were suffered with detachment of Descemet's membrane using a descemetorhexis technique [11 (link)]. In Group B (n = 3), no treatment was applied for the bullous keratopathy postoperatively. In Group C (n = 3), hyperosmolar drops (5.00% NaCl) were instilled in the eyes 4 times daily from the 8th day to the 14th day postoperatively. In Group D (n = 3), glyceraldehyde drops (0.5 M glyceraldehyde (DL-glyceraldehyde, Wako Pure Chemical Industries, Ltd., Osaka, Japan) and 0.02% benzalkonium chloride [BAC, Wako Pure Chemical Industries, Ltd., Osaka, Japan] in 0.90% NaCl) were instilled in the eyes 4 times daily from the 8th day to the 14th day postoperatively. In Group E (n = 3), both hyperosmolar drops and glyceraldehyde drops were combined to be instilled in the eyes 4 times daily from the 8th day to the 14th day postoperatively.
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