Cd4 phycoerythrintexas red x

Manufactured by Beckman Coulter

Sourced in United States

The CD4-PhycoerythrinTexas Red-X is a fluorescence-labeled antibody used for the detection and analysis of CD4+ T cells. It combines the specificity of the CD4 antibody with the fluorescent properties of PhycoerythrinTexas Red-X, allowing for the identification and quantification of CD4-positive cells in flow cytometry applications.

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Lab products found in correlation

Cd45 krome orange clone j 33, by Beckman Coulter (2 mentions) Cd14 pacific blue clone m5e2, by Beckman Coulter (2 mentions)

2 protocols using cd4 phycoerythrintexas red x

Multiparameter Analysis of Immune Cell Subsets

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Different cell populations were labeled with antibodies to CD45 (total leukocytes), CD14 and CD16 (monocytes), CD4 (T helper lymphocytes), CD28 (negative selection for autoreactive T helper lymphocytes), and CD69 (activated lymphocytes).
The antibodies used were the following: CD45-Krome Orange (clone J.33) (CD45-KO), CD4-PhycoerythrinTexas Red-X (Clone SFCI12T4D11 (T4)) (CD4− ECD), CD16-Allophycocyanin-Alexa Fluor 750 (clone 3G8) (CD16-APC-AlexaFluor750) from Beckman Coulter (Miami, FL, USA) and CD14-Pacific Blue (clone M5E2) (CD14-PB), CD28-Pacific Blue (clone CD28.2) (CD28-PB), CD69− Phycoerythrin (clone FN50) (CD69-PE).

Mangas-Losada A., García-García R., Leone P., Ballester M.P., Cabrera-Pastor A., Urios A., Gallego J.J., Martínez-Pretel J.J., Giménez-Garzó C., Revert F., Escudero-García D., Tosca J., Ríos M.P., Montón C., Durbán L., Aparicio L., Montoliu C, & Felipo V. (2019). Selective improvement by rifaximin of changes in the immunophenotype in patients who improve minimal hepatic encephalopathy. Journal of Translational Medicine, 17, 293.

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Multiparametric Flow Cytometry of Immune Cells

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Different cell populations were labeled with antibodies to CD45 (total leukocytes), CD14 and CD16 (monocytes), CD3 (T lymphocytes), CD4 (T helper lymphocytes), CD19 (B lymphocytes), CD28 (negative selection for autoreactive T helper lymphocytes), CD69 (activated lymphocytes) and CD45RA and CD45RO (naive and memory T lymphocytes).
The antibodies used were the following: CD45-Krome Orange (clone J.33) (CD45-KO), CD4-PhycoerythrinTexas Red-X (Clone SFCI12T4D11 (T4)) (CD4- ECD), CD19- Allophycocyanin-Alexa Fluor 700 (clone J3.119) (CD19-APC-AlexaFluor700) and CD16-Allophycocyanin -Alexa Fluor 750 (clone 3G8) (CD16-APC-AlexaFluor750) from Beckman Coulter (Miami, FL, USA) and CD14-Pacific Blue (clone M5E2) (CD14-PB), CD3- Allophycocyanin (clone UCHT1) (CD3-APC), CD28-Pacific Blue (clone CD28.2) (CD28-PB), CD69- Phycoerythrin (clone FN50) (CD69-PE), CD45RA- Peridinin Chlorophyll Protein/Cyanin5.5 (clone HI100) (CD45RA-PERCP/Cy5.5) and CD45RO-Allophycocyanin/Cyanin7 (clone UCHL1) (CD45RO- APC/Cy7) from Biolegend (San Diego, CA, USA).

Mangas-Losada A., García-García R., Urios A., Escudero-García D., Tosca J., Giner-Durán R., Serra M.A., Montoliu C, & Felipo V. (2017). Minimal hepatic encephalopathy is associated with expansion and activation of CD4+CD28−, Th22 and Tfh and B lymphocytes. Scientific Reports, 7, 6683.

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