IMR90 cells that stably expressed KR-TRF1 and DsR-TRF1, respectively, were seeded at a density of 5 × 103 cells per well in 96-well plates 24 h before treatment at the indicated time of light exposure. Cell viability was determined 48 h after light activation with the MTT assay Kit (Promega). Absorbance was measured at 490 nm on a 96-well plate reader (VERSAmax tunable microplate reader, Molecular Devices). Results are presented as percentage of survival, with the control (untreated cells) as 100% survival.
Mtt assay kit
Manufactured by Promega
Sourced in United States
The MTT assay kit is a laboratory tool used for the colorimetric assessment of cell viability and proliferation. The kit contains the necessary reagents to perform the MTT assay, which measures the metabolic activity of cells.
Automatically generated - may contain errors
Lab products found in correlation
Versamax tunable microplate reader, by Molecular Devices (2 mentions)
Synergy ht, by Agilent Technologies (2 mentions)
Microplate reader, by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Prism 7, by GraphPad (1 mentions)
Penicillin streptomycin, by Lonza (1 mentions)
L glutamine, by Lonza (1 mentions)
Calcitriol, by Merck Group (1 mentions)
Plx4720, by Selleck Chemicals (1 mentions)
Raw264, by American Type Culture Collection (1 mentions)
N acetyl cysteine (nac), by Merck Group (1 mentions)
M csf, by R&D Systems (1 mentions)
Fluor chem 8800 imaging system, by Bio-Techne (1 mentions)
Annexin 5 fitc apoptosis detection kit, by Merck Group (1 mentions)
Synergy h1 plate, by Agilent Technologies (1 mentions)
Ap20187, by Merck Group (1 mentions)
Su5416, by Merck Group (1 mentions)
Synergy 4, by Agilent Technologies (1 mentions)
Fk866, by Enzo Life Sciences (1 mentions)
39 protocols using mtt assay kit
1
Light-Induced Cell Viability Assay
2
Assessing Cytotoxic Effects of Artemisia judaica Extract
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Different cancer cell lines, such as prostate (PC-3), breast (MDA-MB-231), ovarian (A2780), and lung (A549) cancer cells lines, were purchased from the National Cancer Institute, Cairo, Egypt. Then, they were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Sigma-Aldrich, St. Louis, MO, USA) and supplemented with 2 mM L-glutamine (Lonza, Belgium), 10% fetal bovine serum (FBS, Sigma-Aldrich, St. Louis, MO, USA), and 1% penicillin-streptomycin (Lonza, Belgium). Cells were plated at a density of 5 × 103 cells in triplicate in 96-well plates. After 48 h, the cells were treated with the ethanolic extract of A. judaica L. at concentrations of 0.1, 1, 10, and 100 µg/mL. Cell viability was assessed after 48 h using the MTT assay kit (Promega, New York, NY, USA) [29 (link)]. An amount of 20 μL of MTT dye, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide, was transferred into the wells, and the plate was incubated for a period of three hours. The absorbance was measured at 570 nm using an ELISA microplate reader (BIO-RAD, model iMark, Tokyo, Japan). The viability was calculated relative to doxorubicin, and the half-maximal inhibitory concentration (IC50) values were determined using the GraphPad prism 7 [30 (link),31 (link)].
3
Cell Proliferation Assay with Calcitriol and PLX4720
4
Cytotoxicity Assay of EGFR Inhibitors
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Both GR and ER cells (approximately 3 × 103 cells/well) were seeded in 96-well plates. Once adherent, the cells were treated with an increasing concentration of either gefitinib or erlotinib in serum-free media. Cell viability was assessed after 72 h using MTT assay kit from Promega (Madison, WI, USA) (cat# G3582) and procedures followed were according to manufacturer’s protocol. Each treatment condition was triplicated. The data were plotted as percentage of cells that are viable ± SEM.
5
Murine Macrophage Osteoclastogenesis Assay
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Murine macrophage cells (RAW 264.7) were purchased from ATCC (Manassas, VA, USA). RAW 264.7 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) containing 10% FBS and 1% penicillin/streptomycin under standard conditions. Cells were seeded in 5 × 104 cells/well in 24-well plates in the presence of RANKL (100 ng/mL; R&D Systems, Minneapolis, MN, USA, #TNSF11) and M-CSF (20 ng/mL; R&D Systems, Minneapolis, MN, USA, #416-ML). Various concentrations of RLE (25, 50, and 100 μg/mL), 100 μM NAC (Sigma-Aldrich, St. Louis, MO, USA, #7250), and 10 μg/mL CX (sigma, #11775) were added to these cultures for 7 days. The culture medium was replaced with fresh medium every 2 days. Osteoclast formation was measured using the TRAP staining kit on day 7. Briefly, adherent cells were fixed with 10% formaldehyde in PBS for 30 min, and TRAP-positive cells with more than three nuclei were scored as osteoclasts. The vitality of cells treated with RLE at concentrations of 25, 50, and 100 μg/mL and other agents was evaluated using MTT assay kit (Promega, Madison, WI, USA, #G4000) by following kit instructions.
6
Cell Viability, Apoptosis, and Colony Formation Assays
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Cell viability was measured 24, 48 and 72 h after transfection with MTT assay kit (Promega, Madison, WI). Flow cytometry analysis for apoptosis was performed using Annexin V-FITC Apoptosis Detection Kit 48 h after transfection according to the manufacturer’s protocol (Sigma–Aldrich). All experiments were performed in triplicate. The colony formation assay was performed as described previously [17 (link)]. In brief, 5 × 104 cells were mixed with methylcellulose (H4100; Stemcell Technologies, Vancouver, BC, Canada) containing RPMI-1640 + 10% FBS and poured in 35 mm plate. The colonies were allowed to grow for 7–14 days, followed by staining with p-Iodonitrotetrazolium violet and counted using Fluorchem 8800 imaging system (Alpha-Innotech, San Leandro, CA).
7
Evaluating NF Cytotoxicity via MTT Assay
8
MTT Assay Using Promega's Kit
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An MTT assay was performed using Promega’s MTT assay kit (Promega, WI, USA) according to the manufacturer’s protocol. Assays were read at 570 nm using a BioTek Synergy H1 plate reader.
9
Cell Proliferation Assay Protocol
10
Cell Viability Assay for Angiogenesis Inhibitors
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Cells were plated 1 × 104 per well in 96-well microtiter plates and incubated in DMEM with 10% FBS for 24 h. Then, either AP20187, SU5416 (Sigma-Aldrich), VEGF-A neutralizing antibody (NAB, R&D Systems, Minneapolis, MN), or vehicle (either ethanol, DMSO, or PBS with control mouse IgG) was added to the media and the cultures were incubated for additional 16 h. Cell viability was determined by the MTT Assay Kit (Promega, Madison, WI) according to the manufacturer's instructions.
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