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Acetylcholinesterase ache from electric eel

Manufactured by Merck Group
Sourced in United States

Acetylcholinesterase (AChE) from electric eel is a purified enzyme derived from the electric organ of the electric eel. AChE is an essential enzyme involved in the breakdown of the neurotransmitter acetylcholine, which plays a crucial role in nerve impulse transmission. This product provides a reliable source of AChE for use in various research and experimental applications.

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2 protocols using acetylcholinesterase ache from electric eel

1

Acetylcholinesterase Inhibition and Antioxidant Assays

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Acetylcholinesterase (AChE) from electric eel (Type VI-S, EC 3.1.17.), acetylthiocholine iodide (ATChI), bovine brain extract (Folch type VII), 3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-4′,4′′-disulfonic acid (ferrozine), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), galantamine hydrobromide, sulfanilamide, and rosmarinic acid (96%) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Aluminium chloride, ethylenediaminetetraacetic acid (EDTA), and hexamethylenetetramine were purchased from Kemika (Zagreb, Croatia). Butylhydroxytoluene (BHT, ≥99%), luteolin (≥97%), N-(1-naphthyl)ethylenediamine, sodium nitroprusside, and trolox (≥98%) were purchased from Fluka (Buchs, Switzerland). Folin-Ciocalteu's phenol reagent and potassium ferricyanide were obtained from Merck (Darmstadt, Germany). All other chemicals and solvents used were of analytical grade.
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2

Efficient Cholinesterase Activity Assay

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Chemicals such as NaCl, chloroform, concentrated sulfuric acid, ferric chloride, lead acetate etc were collected from the general laboratory of the campus. 25 g of AlbuMAX™| Lipid-Rich BSA Cat#11020021(Gibco™ United States) and 5,5′-Dithiobis(2-nitrobenzoic acid) (DTNB; Ellman’s Reagent) Cat# 22582 (Gibco™ United States) were purchased from Thermo Fisher Scientific. Acetylthoicholine chloride (ATCCl) Cat # A5626 (Sigma Aldrich, St. Louis, United States), Gelatin solution Cat # G1393 (Sigma Aldrich, United States), Acetylcholinesterase (AChE) from Electric eel Cat # C3389 (Sigma Aldrich, United States) were purchased from Sigma.
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