Smz1500 stereoscopic microscope

All specimens in this study were dissected to examine the genital structures to aid with identification. Extracted genital structures were dehydrated in absolute alcohol, mounted in Canada balsam on celluloid micro-slides, and pinned with the specimens from which they originated. Images of the entire body and the genital structures were taken using an image processing system (Nikon SMZ 1500 stereoscopic microscope; Nikon Digital Camera DXM 1200F, and Adobe Photoshop software).
Morphological terminology mainly follows that used by Seevers (1978) and Klimaszewski et al. (2011) . The ventral side of the median lobe of the aedeagus is considered to be the side of the bulbus containing the foramen mediale, the entrance of the ductus ejaculatorius, and the adjacent ventral side of the tubus of the median lobe with the internal sac and its structures (this part is referred to as the parameral side in some recent publications); the opposite side is referred to as the dorsal aspect. In species descriptions, microsculpture refers to the surface of the upper forebody (head, pronotum, and elytra).

Observations of morphological characters were made with a SMZ1500 stereoscopic microscope (Nikon, Japan) and a Nikon Eclipse 80i microscope (Nikon, Japan) equipped with differential interference contrast. Fungal structures were mounted in 100% lactic acid. Photographs and measurements were taken with a Nikon DSRi1 camera (Nikon, Japan) and the NIS-Elements D program (Nikon, Japan). Colony characters and pigment production were registered after 2 weeks of growth on PDA, Malt Extract Agar (MEA) and Oatmeal Agar (OA) incubated at 25 °C. Colony colors (obverse and reverse) were assessed according to the color charts of Rayner (1970) . Morphological descriptions were based on cultures sporulating on PDA and pine needles, after 1-month incubation at 25 °C.
Temperature growth studies were performed for the new species described. A 5-mm diameter plug was taken from the margin of an actively growing colony (14-day-old) and placed in the center of PDA, MEA and OA plates. Three replicate plates per isolate were incubated at 10, 15, 20, 25, 30 and 35 °C in the dark. Colony diameter was measured after 1 and 2 weeks.
To evaluate the growth requirements for sea salts, the new species was cultured in PDA with 3% (w/m) sea salts. Three replicate plates per isolate were incubated at 25 °C for 2 weeks in the dark. After incubation the diameter of the colonies was measured and compared.

In order to examine the chemical structure and explain the mechanism of Cu(II) ion sorption on Purolite S 940 and Purolite S 950, the beads were cut through by means of ultramicrotome EM UC7 (Leica) and examined using the optical SMZ 1500 stereoscopic microscope (Nikon). To examine the distribution of elements and the sorbed metal Cu(II) ions, the linear profiles of elemental compositions for the cut beads after the Cu(II) sorption were prepared. In this stage of the study, the scanning electron microscope Quanta 3D FEG with the EDS/EBSD (FEI) system was used.