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2 protocols using potassium chloride (kcl)

1

Antioxidant Activity Evaluation Protocol

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The compounds 2,2′‐azinobis‐(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS), 6‐hydroxy‐2,5,7,8‐tetramethylchroman‐2‐carboxylic acid (Trolox), acetonitrile, formic acid, pepsin (3,200–4,500 units/mg protein), pancreatin (8× USP), and bile salts were obtained from Sigma‐Aldrich (Steinheim, Germany). Acetone, NaHSO3, anhydrous glucose, sodium benzoate, sodium citrate, and potassium phosphate were purchased from Chempur (Piekary Slaskie, Poland). acetonitrile LC‐MS came from POCh (Gliwice, Poland), fructose from Biofan (Piekary Slaskie, Poland), sodium chloride and potassium chloride from STANLAB (Lublin, Poland). Daidzin and genistin were purchased from Extrasynthese (Genay, France).
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2

In Vitro Digestion and Antioxidant Assays

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The compounds 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), formic acid, acetonitrile, pancreatin (8 × USP), pepsin (3200–4500 units/mg protein) and bile salts were obtained from Sigma-Aldrich (Steinheim, Germany). Acetone, anhydrous glucose, NaHSO3, sodium citrate, sodium benzoate, and potassium phosphate were purchased from Chempur (Piekary Slaskie, Poland). acetonitrile LC-MS came from POCh (Gliwice, Poland), fructose from Biofan (Piekary Slaskie, Poland), potassium chloride and sodium chloride from STANLAB (Lublin, Poland). Daidzin and genistin were purchased from Extrasynthese (Genay, France). Inulin Orafti HPX (DP ≥ 23) was purchased from HORTIMEX PLUS (Konin, Poland). Maltodextrin (8 DE) came from the Department of Food Storage and Technology of Wrocław University of Environmental and Life Sciences.
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