Falcon 3ec detector

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Falcon 3EC detector is a compact and versatile detector designed for use in various laboratory applications. It features a high-sensitivity detection system that can accurately measure a wide range of analytes. The Falcon 3EC provides reliable and consistent performance to support your analytical needs.

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Falcon 3EC direct electron detector (28 citations) Falcon 3EC (18 citations)

14 protocols using falcon 3ec detector

Cross-linking and Cryo-EM Structural Analysis of THO-Sub2 Complex

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Purified THO* and Sub2 were first buffer exchanged to 10 mM HEPES pH 7.0, 100 mM potassium acetate, and 0.5 mM TCEP. THO* was incubated with threefold molar excess of Sub2 in the presence of 0.05% glutaraldehyde for 30 min at room temperature. Cross-linking was quenched with 0.1 M Tris pH 8.0 and the sample was concentrated to 0.5 mg/mL. 1.5 µL of THO*•Sub2 was applied to a glow-discharged UltrAuFoil R 1.2/1.3 grids (Quantifoil). Grids were blotted for 3 s with a blotting force of 3 and 100% humidity at 22°C and plunged into liquid ethane using an FEI Vitrobot Mark IV (Thermo Fisher).
Electron micrographs were acquired with a Titan Krios electron microscope (Thermo Fisher) equipped with a Falcon 3EC detector (Thermo Fisher). Movies were collected with EPU with a calibrated pixel size of 0.681 Å/pixel. A total of 4907 movies were collected with a defocus range from 0.8 μm to 2.0 μm. Description of the cryo-EM data collection parameters can be found in Supplementary file 1.

Xie Y., Clarke B.P., Kim Y.J., Ivey A.L., Hill P.S., Shi Y, & Ren Y. (2021). Cryo-EM structure of the yeast TREX complex and coordination with the SR-like protein Gbp2. eLife, 10, e65699.

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Cryo-EM Data Acquisition Protocol

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Images were acquired on a Titan Krios cryo-EM (Thermo Fisher Scientific, Waltham, MA, USA) at 300 kV equipped with a Cs corrector (CEOS, GmbH), which was installed in the Institute for Protein Research, Osaka University. Data were acquired automatically with EPU software as movies on a Falcon 3EC detector (Thermo Fisher Scientific, Waltham, MA, USA). The detailed imaging condition is described in Supplementary Table 4.

Fujita-Fujiharu Y., Sugita Y., Takamatsu Y., Houri K., Igarashi M., Muramoto Y., Nakano M., Tsunoda Y., Taniguchi I., Becker S, & Noda T. (2022). Structural insight into Marburg virus nucleoprotein–RNA complex formation. Nature Communications, 13, 1191.

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Cryo-EM Sample Preparation Workflow

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C-flat™ 2/2, 200 mesh holey carbon grids (Protochips, Morrisville, NC, USA) were glow-discharged for 60 s at 20 mA, air atmosphere, positive polarity (GloQube ^® Plus, Quorum, Laughton, UK). We applied 3 µL of sample to the grid. Then, the sample was blotted and vitrified in liquid ethane on Vitrobot Mark IV (Thermo Fisher Scientific, Waltham, MA, USA). Vitrobot conditions were as follows: 100% relative humidity, blot force: 2, blot time: 7 s and 4 °C. Samples were visualized under cryogenic conditions. We used a 200 kV Glacios microscope with Falcon 3EC detector (Thermo Fisher Scientific, Waltham, MA, USA).

Spasovski V., Romolo A., Zagorc U., Arrigler V., Kisovec M., Bedina Zavec A., Arko M., Molnár A., Schlosser G., Iglič A., Kogej K, & Kralj-Iglič V. (2024). Characterization of Nanohybridosomes from Lipids and Spruce Homogenate Containing Extracellular Vesicles. International Journal of Nanomedicine, 19, 1709-1721.

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Cryo-TEM Sample Preparation Protocol

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Samples for the cryo-TEM were prepared by using the Vitrobot Mark IV (Thermo Fisher Scientific, Waltham, MA, USA). Quantifoil^® R 2/2, 200 (Quantifoil Micro Tools GmbH, Großlöbichau, Germany) or C-Flat 2/2, 200 mesh (Protochips, Morrisville, NC, USA) holey carbon grids were glow discharged for 60 s at 20 mA and positive polarity in air atmosphere (GloQube^® Plus, Quorum, Laughton, UK). Vitrobot conditions were set to 4 °C, 95% relative humidity, Blot time 3 s and Blot force 1. Then, 2 µL of the suspension was applied to the grid, blotted and vitrified in liquid ethane. Excess liquid was removed by filter paper. Samples were visualized with a 200 kV microscope Glacios with a Falcon 3EC detector (Thermo Fisher Scientific, Waltham, MA, USA).

Mammadova R., Fiume I., Bokka R., Kralj-Iglič V., Božič D., Kisovec M., Podobnik M., Zavec A.B., Hočevar M., Gellén G., Schlosser G, & Pocsfalvi G. (2021). Identification of Tomato Infecting Viruses That Co-Isolate with Nanovesicles Using a Combined Proteomics and Electron-Microscopic Approach. Nanomaterials, 11(8), 1922.

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Structural Characterization of SARS-CoV-2 Spike-Antibody Complex

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SARS-CoV-2 HexaPro spike (GnT1- produced) was incubated with 20-fold excess of CV3-13 Fab overnight at 4°C before purification on a Superose 6 300/10 GL column (GE Healthcare). The complex peak was harvested, concentrated to 0.50 mg/mL in the SEC buffer and immediately used for Cryo-EM grid preparation. 3μL of protein was deposited on a holey copper grids (QUANTIFOIL R 1.2/1.3, 200 mesh, EMS) which had been glow-discharged for 30s at 15 Ma using PELCO easiGlow (TedPella Inc). Grids were vitrified in liquid ethane using Vitrobot Mark IV (Thermo Fisher) with a blot time of 2–4 s and variable blot force at 4°C and 100% humidity.
The frozen grids were screened on a FEI Talos Arctica microscope at 200 kV equipped with a FEI Falcon3EC detector using the EPU software (Thermo Fisher). Cryo-EM data from a good grid were acquired on a FEI Glacios electron microscope operating at 200 kV, equipped with a Gatan K3 direct electron detector. Micrographs were collected at a magnification of 45,000 corresponding to a calibrated pixel size of 0.8893 Å, with a total exposure dose of 42 e-/ Å².

Beaudoin-Bussières G., Chen Y., Ullah I., Prévost J., Tolbert W.D., Symmes K., Ding S., Benlarbi M., Gong S.Y., Tauzin A., Gasser R., Chatterjee D., Vézina D., Goyette G., Richard J., Zhou F., Stamatatos L., McGuire A.T., Charest H., Roger M., Pozharski E., Kumar P., Mothes W., Uchil P.D., Pazgier M, & Finzi A. (2022). A Fc-enhanced NTD-binding non-neutralizing antibody delays virus spread and synergizes with a nAb to protect mice from lethal SARS-CoV-2 infection. Cell Reports, 38(7), 110368.

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DNA Origami Visualization via Cryo-EM

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The purified and concentrated DNA origami samples (3 μl) were applied to glow-discharged copper C-flat thick R2/1 300-mesh grids and frozen in liquid ethane using a Vitrobot (Thermo Fisher Scientific) with 3-s blot. Grids were then imaged on a Talos Arctica microscope (Thermo Fisher Scientific) with a Falcon 3EC detector, operated at 200 kV and ×57,000 magnification (2.54-Å nominal pixel size), using EPU software (Thermo Fisher Scientific) to collect micrographs.

Wang X., Li S., Jun H., John T., Zhang K., Fowler H., Doye J.P., Chiu W, & Bathe M. (2022). Planar 2D wireframe DNA origami. Science Advances, 8(20), eabn0039.

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Cryo-EM of Purified HIV-1 Env Liposomes

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Vitrification of purified HIV-1 Env liposomes was done using a Vitrobot Mark IV (Thermo Fisher Scientific). 3 µL sC4-CoP1, sC4-CoP2, or sC4-CoP3 sample was applied onto freshly glow discharged Quantifoil R2/2 copper grids, blotted for 3 seconds at 22 °C and 100% relative humidity, and plunge frozen in liquid ethane. Micrographs were collected on a Titan Krios operated at 300 kV, equipped with a Falcon 3EC detector (Thermo Fisher Scientific) at a nominal magnification of 19500 x (4.4 angstrom per pixel) at The Netherlands Center for Electron Nanoscopy (NeCEN). Two representative images per grid were selected from a single experiment.

Koornneef A., Vanshylla K., Hardenberg G., Rutten L., Strokappe N.M., Tolboom J., Vreugdenhil J., Boer K.F., Perkasa A., Blokland S., Burger J.A., Huang W.C., Lovell J.F., van Manen D., Sanders R.W., Zahn R.C., Schuitemaker H., Langedijk J.P, & Wegmann F. (2024). CoPoP liposomes displaying stabilized clade C HIV-1 Env elicit tier 2 multiclade neutralization in rabbits. Nature Communications, 15, 3128.

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Cryo-EM Sample Preparation for Structural Analysis

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C-flat™ 2/2, 200 mesh holey carbon grids (Protochips, Morrisville, NC, USA) were glow discharged: 20 mA, 60 s, positive polarity, air atmosphere (GloQube^® Plus, Quor-um, Laughton, UK). Then, 3 µL of the sample was applied to the grid, blotted, and vitrified in liquid ethane on Vitrobot Mark IV (Thermo Fisher Scientific, Waltham, MA, USA). Vitrobot conditions were set to 100% relative humidity, 4 °C, blot force: 2 and blot time: 7 s. Samples were visualized under cryogenic conditions using a 200 kV Glacios microscope with a Fal-con 3EC detector (Thermo Fisher Scientific, Waltham, MA, USA).

Jeran M., Romolo A., Spasovski V., Hočevar M., Novak U., Štukelj R., Šuštar V., Kisovec M., Bedina Zavec A., Kogej K., Iglič A., Trebše P, & Kralj-Iglič V. (2023). Small Cellular Particles from European Spruce Needle Homogenate. International Journal of Molecular Sciences, 24(5), 4349.

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Cryo-EM Grid Preparation Workflow

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Quantifoil^® R 2/2 (or 1.2/1.3), 200 (Quantifoil Micro Tools GmbH, Großlöbichau, Germany) EM grids were glow discharged for 60 s at 20 mA and positive polarity in air atmosphere (GloQube^® Plus, Quorum, Laughton, UK). Vitrobot conditions were set to 4 °C, 95% relative humidity, blot time: 5 s, and blot force: 4. An amount of 2 µL of the sample suspension was applied to the grid, blotted, and plunge-frozen in liquid ethane with Vitrobot Mark IV (Thermo Fisher Scientific, Waltham, MA, USA). Samples were visualized under cryo conditions with a Falcon 3EC detector on a 200 kV microscope Glacios (Thermo Fisher Scientific, Waltham, MA, USA).

Mantile F., Kisovec M., Adamo G., Romancino D.P., Hočevar M., Božič D., Bedina Zavec A., Podobnik M., Stoppelli M.P., Kisslinger A., Bongiovanni A., Kralj-Iglič V, & Liguori G.L. (2022). A Novel Localization in Human Large Extracellular Vesicles for the EGF-CFC Founder Member CRIPTO and Its Biological and Therapeutic Implications. Cancers, 14(15), 3700.

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Cryo-EM Sample Preparation for Structural Biology

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Samples of SCPs were prepared using Vitrobot Mark IV (Thermo Fisher Scientific, Waltham, MA, USA). Quantifoil^®® R 2/2, 200 mesh holey carbon grids (Quantifoil Micro Tools GmbH, Großlöbichau, Germany) were glow-discharged for 60 s at 20 mA and positive polarity in the air (GloQube^®® Plus, Quorum, Laughton, UK) [55 (link)]. Conditions were set at 4 °C, 100% relative humidity, blot time: 5 s, and blot force: 4. An amount of 2 µL of the sample with SCPs in suspension was applied to the grid, blotted, and vitrified in liquid ethane. Samples were visualized under cryogenic conditions using a 200 kV microscope Glacios with Falcon 3EC detector (Thermo Fisher Scientific, Waltham, MA, USA). The total electron dose was 30 eA⁻².

Škufca D., Božič D., Hočevar M., Jeran M., Bedina Zavec A., Kisovec M., Podobnik M., Matos T., Tomazin R., Iglič A., Griessler Bulc T., Heath E, & Kralj-Iglič V. (2022). Interaction between Microalgae P. tricornutum and Bacteria Thalassospira sp. for Removal of Bisphenols from Conditioned Media. International Journal of Molecular Sciences, 23(15), 8447.

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