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Lc200 cu 100

Manufactured by Ted Pella
Sourced in United States

The LC200-CU-100 is a laboratory equipment product manufactured by Ted Pella. It is a core tool designed for specific laboratory functions, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

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2 protocols using lc200 cu 100

1

Cryo-EM Sample Preparation Technique

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Prior to plunge-freezing, 200 mesh Cu grids with a lacey carbon membrane (EMS Cat# LC200-CU-100) were glow discharged in a Pelco easiGlow glow discharger (Ted Pella) using an atmosphere plasma generated at 15 mA for 15 s with a pressure of 0.24 mbar. This treatment created a negative charge on the carbon membrane, allowing liquid samples to spread evenly over the grid. Four microliters of the sample was pipetted onto the grid and blotted for 5 s with a blot offset of +0.5 mm, followed by immediate plunging into liquid ethane within a FEI Vitrobot Mark III plunge freezing instrument (Thermo Fisher Scientific). Grids were then transferred to liquid nitrogen for storage. The plunge-frozen grids were kept vitreous at –180 °C in a Gatan Cryo Transfer Holder model 626.6 (Gatan) while viewing in a JEOL JEM1230 LaB6 emission TEM (JEOL USA) at 100 keV. Image data were collected by a Gatan Orius SC1000 CCD camera Model 831 (Gatan).
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2

Cryo-TEM Specimen Preparation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The cryo-TEM
data were obtained at the BioCryo facility of the Northwestern University
Nuance Center (remote access due to COVID19 restrictions). The samples
were applied to 200 mesh Cu grids with a lacey carbon membrane. Before
plunge-freezing, grids (EMS Cat# LC200-CU-100) were glow discharged
in a Pelco easiGlow glow discharger (Ted Pella Inc., Redding, CA,
USA) using an atmosphere plasma generated at 15 mA for 15 s with a
pressure of 0.24 mbar. This treatment created a negative charge on
the carbon membrane, allowing liquid samples to spread evenly over
the grid. A 4 μL volume of each sample was pipetted onto the
grid and blotted for 5 s with a blot offset of +0.5 mm, followed by
immediate plunging into liquid ethane within an FEI Vitrobot Mark
III plunge freezing instrument (Thermo Fisher Scientific, Waltham,
MA, USA). Grids were then transferred to liquid nitrogen for storage.
The plunge-frozen grids were kept vitreous at −180 °C
in a Gatan Cryo Transfer Holder model 626.6 (Gatan Inc., Pleasanton,
CA, USA) while viewing using a Hitachi HT7700 W-emission transmission
electron microscope at 100 kV. Image data were collected by a Gatan
Orius 4k × 2.67k digital camera (Gatan Inc., Pleasanton, CA,
USA). Samples required no dilution at 1 wt % concentration for successful
visualization. Cryo-TEM images were analyzed using ImageJ software.
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