Supernatants from co-culture assays were collected after 72 h and stored at −80°C until cytokine quantification by sandwich ELISA. Briefly, 96-well flat-bottomed plates were coated overnight with 1 μg/mL purified anti-mouse IL-10 (clone JES5-2A5, Biolegend) capture antibody. After several washing steps with 1X PBS + 0.05% Tween 20 and a blocking step with 1% BSA in 1X PBS, samples were incubated at room temperature for 2 h. Biotinylated anti-mouse IL-10 (clone JES5-16E3, Biolegend) at 1 μg/mL in conjunction with HRP-avidin (Biolegend) were used for detecting immobilized cytokine and tetramethylbenzidine (TMB, ThermoFisher) substrate was added to detect HRP activity. Reaction was stopped by adding 2N H2SO4 and absorbance was measured at 450 nm wavelength using a Tecan absorbance microplate reader.
Anti mouse il 10 clone jes5 16e3
Manufactured by BioLegend
Anti-mouse IL-10 (clone JES5-16E3) is a monoclonal antibody that binds to the mouse interleukin-10 (IL-10) cytokine. It can be used for the detection and quantification of mouse IL-10 in various immunoassays.
Automatically generated - may contain errors
2 protocols using anti mouse il 10 clone jes5 16e3
1
Quantification of IL-10 by ELISA
2
IgA Production by Intestinal Phagocytes
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