Continuous-wave (cw) X-band EPR measurements
were performed on a Bruker
E500 ELEXSYS spectrometer equipped with
the Bruker dual-mode cavity (
ER4116DM) or a standard cavity (ER4102ST)
and an Oxford Instruments
helium flow cryostat (ESR 900). The microwave
bridge was a high-sensitivity Super-X bridge (Bruker
ER-049X) with
integrated microwave frequency counter. The magnetic field controller
(ER032T) was calibrated with a Bruker
NMR field probe (ER035M). EPR
simulations have been done with our own routines, esim_gfit and esim_sx.
For spin quantitation, the experimental derivative spectra were numerically
integrated by using the routine eview, and the results were corrected
for their
g value dependence for field-swept spectra
by using Aasa and Vänngård approximation,
32 (link) i.e. dividing the integrals by the factor,
Chang H.C., Mondal B., Fang H., Neese F., Bill E, & Ye S. (2019). Electron Paramagnetic Resonance Signature of Tetragonal Low Spin Iron(V)-Nitrido and -Oxo Complexes Derived from the Electronic Structure Analysis of Heme and Non-Heme Archetypes. Journal of the American Chemical Society, 141(6), 2421-2434.