Cmir sn0001 sn

The pLKO.1-puro vectors encoding a non-targeting control (NTC) short hairpin RNA (shRNA) or shRNA targeting either HIF-1α (shHIF-1α), HIF-2α (shHIF-2α) or HDAC3 (shHDAC3) were purchased from Sigma-Aldrich. Then lentivirals were constructed. The pcDNA3.1/HDAC3 vector (GenePharma, Shanghai, China) was applied to overexpress HDAC3. And the empty pcDNA3.1 vector was used for the control. The miR-627-5p mimics (#HmiR-SN0735) and miRNA scrambled control (# CmiR-SN0001-SN) were purchased from FulenGen (Guangzhou, China). All of the experimental operations were based on the product specifications.

Caco-2 cells were treated with TNF-α (Sigma, United States) at final concentrations of 0, 50, 100, and 150 ng/ml, respectively. In some experiments, Caco-2 cells were stimulated with parthenolide (p65 inhibitor, purchased from TOCRIS) at a final concentration of 5 μmol/L.
Caco-2 cells were transfected with miRNAs or plasmid using Lipofectamine 2000 reagent (Invitrogen). The miR-21-3p mimic (HmiR-SN0315), miR control (CmiR-SN0001-SN), miR-21-3p inhibitor (HmiR-AN0315-SN-10), and miR inhibitor control (CmiR-AN0001-SN) were purchased from FulenGen (Guangzhou, China). The sequence of siRNA that specifically targeted MTDH was chemically synthesized by Sangon (Shanghai, China) (forward: GCA​AUU​CCU​UGG​AUC​UUA​UTT, reverse: AUA​AGA​UCC​AAG​GAA​UUG​CTT).