Ginkgetin, berberine, 1,1′‐Dioctadecyl‐3,3,3′,3′‐tetramethylindocarbocyanine perchlorate (DiI) and lipopolysaccharide (LPS) from E. coli were purchased from Sigma‐Aldrich (St. Louis, MO) and dissolved in DMSO as stock solutions. RNeasy micro kit and RNase‐free DNase were obtained from Qiagen (Germantown, MD). Primers for TNFα, CCL2, IL6 and CD36, and iTaq Universal SYBR Green RT‐qPCR kit were obtained from Bio‐Rad (Hercules, CA). Thioglycollate was purchased from Beckton‐Dickinson (Franklin Lakes, NJ). Exosome precipitating reagent ExoQuick‐TC and Fluorocet kit were from System Biosciences (Palo Alto, CA). Phosphate buffered saline (PBS), Dulbecco's Modified Eagle Medium (DMEM), heat‐inactivated foetal bovine serum (FBS), native low‐density lipoprotein (LDL), bovine serum albumin (BSA) and cell culture related reagents were purchased from Thermo Fisher Scientific (Waltham, MA). All other chemicals were purchased from Sigma‐Aldrich or Thermo Fisher Scientific.
Fluorocet kit
Manufactured by System Biosciences
The Fluorocet kit is a laboratory tool designed for the detection and measurement of fluorescent proteins. It provides the necessary components for accurate and reliable fluorescence analysis.
Automatically generated - may contain errors
Lab products found in correlation
Exoquick tc, by System Biosciences (2 mentions)
Rneasy micro kit, by Qiagen (1 mentions)
Thioglycollate, by BD (1 mentions)
Berberine, by Merck Group (1 mentions)
Cell culture related reagents, by Thermo Fisher Scientific (1 mentions)
Lipopolysaccharide, by Merck Group (1 mentions)
Rnase free dnase, by Qiagen (1 mentions)
Ginkgetin, by Merck Group (1 mentions)
Lsrfortessa x 20, by BD (1 mentions)
Fluoroprofile protein quantification kit, by Merck Group (1 mentions)
Exoflow, by System Biosciences (1 mentions)
Quick start bradford protein assay, by Bio-Rad (1 mentions)
Ns300, by Malvern Panalytical (1 mentions)
Ripa lysis buffer, by Merck Group (1 mentions)
4 protocols using fluorocet kit
1
Lipid Regulation and Inflammation Modulation
2
Exosome Isolation and Characterization from BAL Fluid
3
Exosome Characterization Techniques
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The number and size of the EV particles were evaluated using nanoparticle tracking analysis methods on a NanoSight NS-300 (Malvern), instrument per manufacturer’s instructions. Acetylcholinesterase (AChE) enzymatic assays were performed using fluorescent enzymatic assays (Fluorocet kit, SBI). The protein quantification was performed using the Quick Start™ Bradford Protein Assay (Bio-Rad). The exosome RNA concentration was determined by NanoDrop UV spectroscopy (Thermo Scientific).
4
Exosome Quantification and Characterization
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Parts of the exosome samples were lysed with RIPA lysis buffer (Merck, Rahway, NJ, USA) and protein concentrations in exosome lysates were determined with a BCA protein assay (Pierce, Appleton, WI, USA). Exosome quantification in samples was carried out with a Fluorocet kit (SBI, System Biosciences). The presence of exosomes in samples was also confirmed by Western blot assay.
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