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Mpeg2000 deg

Manufactured by NOF corporation
Sourced in Japan

The MPEG2000-DEG is a specialized laboratory equipment designed for digital signal processing and analysis. It is capable of encoding and decoding MPEG-2 video streams. The core function of this device is to facilitate the conversion and evaluation of multimedia data.

Automatically generated - may contain errors

3 protocols using mpeg2000 deg

1

Regulation of Inflammatory Pathways

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LPS and nigericin were purchased from Sigma-Aldrich (St. Louis, MO). NF-kB inhibitor, QNZ (EVP4593), was supplied by MedChemExpress (New Jersey, USA). Antibodies against FTO, NLRP3, FoxO1, P65, p-P65, IL-1β and Cleaved-IL-1β (Asp117) were obtained from Cell Signaling Technologies (Beverly, MA). ELISA kits of IL-1β, interleukin-6 (IL-6), interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), interleukin-10 (IL-10) and interleukin-12(p70) (IL-12(p70)) were purchased from eBioscience (San Diego, CA). Brilliant Violet 421™ anti-mouse F4/80 antibody, PE anti-mouse/human CD11b antibody, FITC anti-mouse I-A/I-E antibody, APC anti-mouse CD80 antibody, PE/Cy7 anti-mouse CD86 antibody, FITC anti-mouse Ly-6G antibody and APC anti-mouse CD40 antibody were obtained from BioLegend (San Diego, CA, USA). Lipidoid (C12-200) was supplied by Xinjiahecheng Medical Chemistry Corporation (Wuhan, Hubei, China). mPEG2000-DEG was purchased from NOF Corporation (Tokyo, Japan).
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2

Nanoparticle-Mediated Delivery of Mecp2 siRNA

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Bleomycin (BLM) was obtained from Huirui. Murine recombinant IL‐4 was purchased from PeproTech. The Lipofectamine 3000 transfection kit was acquired from Invitrogen. Cholesterol and DSPC were purchased from Sigma‐Aldrich, Inc. Lipidoid (C12‐200) was acquired from Xinjiahecheng Medical Chemistry Corporation. mPEG2000‐DEG was purchased from NOF Corporation. siRNAs targeting Mecp2 and Scr siRNA were purchased from Guangzhou RiboBio Co., Ltd.
Antibodies against CD68, CD206, and TGF‐β1 were purchased from Santa Cruz Biotechnology. Antibodies against arginase‐1 and fibronectin were purchased from Abcam. Antibodies against Mecp2, IRF4, inducible nitric oxide synthase (iNOS), and α‐SMA were purchased from Cell Signaling Technology. Antibodies against collagen I, Irf4, Gapdh, and β‐actin were purchased from Proteintech, and antibodies against Ym1 were purchased from Thermo Fisher Scientific. APC‐conjugated anti‐mouse F4/80, PE‐conjugated anti‐mouse CD11c, and FITC‐conjugated anti‐mouse CD206 antibodies were purchased from BioLegend.
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3

Murine Recombinant IL-4 Signaling Pathway

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Murine recombinant IL-4 was obtained from PeproTech (London, UK). Antibodies against collagen type I was purchased from EMD Millipore (Schwalbach, Germany). Antibodies against fibronectin, α-SMA, arginase-1, CD68, MBD2, and pro-SPC were purchased from Abcam (Cambridge, MA, USA), and antibodies against p-Smad2, p-Smad3, STAT6, P85, p-P85, and p-Akt (Thr308) were obtained from Cell Signaling Technology (Danvers, MA, USA). Antibodies against MBD2, glyceraldehyde-3-phosphate dehydrogenase, p-Akt (Ser473), and p-STAT6 were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-mouse F4/80-PerCP/Cy5.5 and CD206–fluorescein isothiocyanate (FITC) were from BioLegend (San Diego, CA, USA), and CD11c–allophycocyanin (APC) were purchased from BD Biosciences (San Jose, CA, USA). Lipidoid (C12-200) was purchased from Xinjiahecheng Medical Chemistry Corporation (Wuhan, Hubei, China). mPEG2000-DEG was purchased from NOF Corporation (Tokyo, Japan). All other reagents were purchased from Sigma-Aldrich (St Louis, MO, USA), unless otherwise stated.
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