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4 protocols using anti rabbit igg 711 035 152

1

Antibody Panel for Lipid Metabolism Analysis

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The antibodies anti-RAB18 (11308-1-AP), anti-PLIN2 (15294-1-AP), anti-HSPA8 (10654-1-AP), and anti-NPC1 (13926-1-AP) were purchased from Proteintech; anti-LAMP1 (#9091), anti-GM130 (#12480), and anti-RELA (#3033) were purchased from Cell Signaling Technology; anti-LAMP2A (ab18528) was purchased from Abcam; anti-HA (A01244) and anti-His6 (C157) were purchased from Genscript; anti-FLAG (F1804) and anti-ATCB (A1978) were purchased from Sigma-Aldrich; anti-PRRSV-2 nucleocapsid (N) (SDOW17) was purchased from Rural Technologies; horseradish peroxidase (HRP)-conjugated donkey anti-mouse IgG (715-035-150) and anti-rabbit IgG (711-035-152) antibodies were purchased from Jackson ImmunoResearch Laboratories; anti-rabbit IgG antibodies labeled with Alexa Fluor 488 (A11034) and Alexa Fluor 568 (A11011) and anti-mouse IgG antibodies labeled with Alexa Fluor 488 (A11001), Alexa Fluor 568 (A11004), Alexa Fluor 405 (A31553), and Alexa Fluor Plus 647 (A32728) were purchased from Thermo Fisher Scientific. The antibodies described above were used at dilutions of 1:500 for immunofluorescence and 1:1,000 for immunoblot analysis.
Oil Red O (O0625) was purchased from Sigma-Aldrich. Chloroquine (HY-17589A) was purchased from MedChemExpress. BODIPY 493/503 (D3922) and DAPI (D1306) were purchased from Thermo Fisher Scientific.
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2

Antibody Sources and Dilutions for Immunoassays

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The antibodies anti-LC3 (#4599), anti-P62 (#5114), anti-ACC (#3676), and antiphosphorylated ACC (#11818) were ordered from Cell Signaling Technology (MA, USA); anti-GAPDH (10494-1-AP) was ordered from Proteintech (Wuhan, China); anti-FASN (ab22759) was ordered from Abcam (MA, USA); anti-HMGCR (MABS1233) was ordered from Sigma-Aldrich; anti-phosphorylated HMGCR (bs-4063R) was ordered from Bioss (MA, USA); anti-PRRSV nucleocapsid (N) SDOW17 was ordered from Rural Technologies (WA, USA); anti-dsRNA (J2 and K1) was ordered from English & Scientific Consulting (Szirak, Hungary); horseradish peroxidase (HRP)-conjugated donkey anti-mouse IgG (715-035-150) and anti-rabbit IgG (711-035-152) were ordered from Jackson ImmunoResearch Laboratories (West Grove, PA, USA); and anti-mouse IgG labeled with Alexa Fluor 555 (A21424), Alexa Fluor 488 (A21429), and anti-rabbit IgG labeled with Alexa Fluor 488 (A11034) were ordered from Thermo Fisher Scientific. The aforementioned antibodies were used at dilutions of 1:500 for immunofluorescence staining and 1:1,000 for immunoblotting analysis.
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3

Reagents and Antibodies for Biochemical Assays

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We obtained TRIzol Reagent (D9108B) and SYBR Premix Ex Taq (RR420A) from TaKaRa; a Cell Counting Kit-8 (CCK-8, ZP328) from Zoman Bio (Beijing, China); a malondialdehyde (MDA) test kit (A003-4) from Jian Cheng (Nanjing, China); mouse monoclonal anti-actin (A1978) from Sigma; Lycopene (HY–N0287) and methylene blue (HY-14536) from MedChemExpress; Singlet Oxygen Sensor Green (S36002) from Invitrogen; deuterium oxide (D2O, D113904) from Aladdin (Shanghai, China). Anti-glycoprotein E (gE) [30 ] was a gift from Professor Ke-Gong Tian (Henan Agricultural University, China). Antisera against PRV glycoprotein B (gB) were generated by immunization of mice with purified recombinant gB. Horseradish-peroxidase-conjugated donkey anti-mouse IgG (715-035-150) and anti-rabbit IgG (711-035-152) antibodies were from Jackson ImmunoResearch Laboratories; anti-mouse IgG antibody labeled with Alexa Fluor 488 (A21429) was from Thermo Fisher Scientific. The antibodies described above were used at dilutions of 1:500 for immunofluorescence staining and 1:1,000 for immunoblotting. MDA (BWQ7504-2016) was from Beijing Northern Weiye Institute of Metrology and Technology. LJ002 and LJ001 were synthesized as previously described [27 (link)].
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4

Antibody Sources and Dilutions for Immunoassays

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The antibodies anti-LC3 (#4599), anti-P62 (#5114), anti-ACC (#3676), and antiphosphorylated ACC (#11818) were ordered from Cell Signaling Technology (MA, USA); anti-GAPDH (10494-1-AP) was ordered from Proteintech (Wuhan, China); anti-FASN (ab22759) was ordered from Abcam (MA, USA); anti-HMGCR (MABS1233) was ordered from Sigma-Aldrich; anti-phosphorylated HMGCR (bs-4063R) was ordered from Bioss (MA, USA); anti-PRRSV nucleocapsid (N) SDOW17 was ordered from Rural Technologies (WA, USA); anti-dsRNA (J2 and K1) was ordered from English & Scientific Consulting (Szirak, Hungary); horseradish peroxidase (HRP)-conjugated donkey anti-mouse IgG (715-035-150) and anti-rabbit IgG (711-035-152) were ordered from Jackson ImmunoResearch Laboratories (West Grove, PA, USA); and anti-mouse IgG labeled with Alexa Fluor 555 (A21424), Alexa Fluor 488 (A21429), and anti-rabbit IgG labeled with Alexa Fluor 488 (A11034) were ordered from Thermo Fisher Scientific. The aforementioned antibodies were used at dilutions of 1:500 for immunofluorescence staining and 1:1,000 for immunoblotting analysis.
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