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Purified recombinant hldhb 6375

Manufactured by Abcam
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Purified recombinant hLDHB (6375) is a laboratory reagent produced by Abcam. It is a recombinant form of the human lactate dehydrogenase B (LDHB) protein, which is involved in the conversion of lactate to pyruvate in cellular metabolism. The product is purified and provided in a standardized format for use in research and scientific applications.

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Lab products found in correlation

Adenosine 5 diphosphate, by Merck Group (2 mentions) Tepp 46, by Aobious (2 mentions) Phosphoenolpyruvic acid monopotassium salt b20358, by Thermo Fisher Scientific (2 mentions) 13c6 glucose clm 1396 13c5 glutamine clm 1822 3 3 3 d3 lactate dlm 9071 3 13c pyruvate clm 1585, by Cambridge Isotopes (2 mentions) 13c3 pyruvate clm 2440, by Cambridge Isotopes (2 mentions) Mtbstfa 1 tbdmcs ts 48927, by Thermo Fisher Scientific (2 mentions) Purified recombinant hldha 6374, by Abcam (2 mentions)

2 protocols using purified recombinant hldhb 6375

1

Metabolic Flux Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
DASA was purchased from EMD Millipore (550602) and TEPP-46 was purchased from Aobious (AOB1123). Pyridoxamine dihydrochloride (P9380), beta-nicotinamide adenine dinucleotide, reduced (N8129), 13C6 citrate (606081), oxaloacetic acid (O4126), l-aspartic acid (A9256), methoxamine (M6524), pyridine (270970) and adenosine 5-diphosphate (A2754) were purchased from Sigma. Phosphoenolpyruvic acid monopotassium salt (B20358) was purchased from Alfa Aesar. 13C6 glucose (CLM-1396), 13C5 glutamine (CLM-1822), 3,3,3-D3 lactate (DLM-9071), 3-13C pyruvate (CLM-1585), and 13C3 pyruvate (CLM-2440) were purchased from Cambridge Isotope Laboratories. D-glucose (50146244), dimethylformamide (P120673), MSTFA + 1% TMCS (TS-48915) and MTBSTFA + 1% TBDMCS (TS-48927) were purchased from Fisher. Purified recombinant hLDHA (6374) and purified recombinant hLDHB (6375) were purchased from Biovision. Purified rbLDHA was purchased from VWR EMD (80601).
Wiese E.K., Hitosugi S., Loa S.T., Sreedhar A., Andres-Beck L.G., Kurmi K., Pang Y.P., Karnitz L.M., Gonsalves W.I, & Hitosugi T. (2021). Enzymatic Activation of Pyruvate Kinase Increases Cytosolic Oxaloacetate to Inhibit the Warburg Effect. Nature metabolism, 3(7), 954-968.
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2

Metabolic Flux Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
DASA was purchased from EMD Millipore (550602) and TEPP-46 was purchased from Aobious (AOB1123). Pyridoxamine dihydrochloride (P9380), beta-nicotinamide adenine dinucleotide, reduced (N8129), 13C6 citrate (606081), oxaloacetic acid (O4126), l-aspartic acid (A9256), methoxamine (M6524), pyridine (270970) and adenosine 5-diphosphate (A2754) were purchased from Sigma. Phosphoenolpyruvic acid monopotassium salt (B20358) was purchased from Alfa Aesar. 13C6 glucose (CLM-1396), 13C5 glutamine (CLM-1822), 3,3,3-D3 lactate (DLM-9071), 3-13C pyruvate (CLM-1585), and 13C3 pyruvate (CLM-2440) were purchased from Cambridge Isotope Laboratories. D-glucose (50146244), dimethylformamide (P120673), MSTFA + 1% TMCS (TS-48915) and MTBSTFA + 1% TBDMCS (TS-48927) were purchased from Fisher. Purified recombinant hLDHA (6374) and purified recombinant hLDHB (6375) were purchased from Biovision. Purified rbLDHA was purchased from VWR EMD (80601).
Wiese E.K., Hitosugi S., Loa S.T., Sreedhar A., Andres-Beck L.G., Kurmi K., Pang Y.P., Karnitz L.M., Gonsalves W.I, & Hitosugi T. (2021). Enzymatic Activation of Pyruvate Kinase Increases Cytosolic Oxaloacetate to Inhibit the Warburg Effect. Nature metabolism, 3(7), 954-968.
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