The human CRC cell lines HCT116, RKO, LoVo, DLD‐1, SW480, and SW620 were obtained from the JCRB Cell Bank or ATCC. HCT116 p53(−/−) cells were purchased from Horizon Discovery. All cells were maintained in DMEM (Nacalai Tesque) supplemented with 10% FBS (Biowest, Nuaillé, France) and 1% penicillin–streptomycin (Nacalai Tesque). Cells were cultured at 37°C in a 5% CO2 incubator.
Hct116 p53 cells
Manufactured by Horizon Discovery
Sourced in United Kingdom
The HCT116 p53(-/-) cells are isogenic cell lines derived from the human colorectal carcinoma cell line HCT116, with both alleles of the p53 gene knocked out. These cells lack functional p53 protein, making them a valuable tool for studying the role of p53 in various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Dld 1, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Sw620, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Sw480, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Hct116, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Penicillin streptomycin, by Nacalai Tesque (1 mentions)
Dulbecco modified eagle medium (dmem), by Nacalai Tesque (1 mentions)
Fetal bovine serum (fbs), by Biowest (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Hct116 p53wt, by American Type Culture Collection (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
2 protocols using hct116 p53 cells
1
Maintenance of Human CRC Cell Lines
2
Culturing HCT116 Colorectal Cancer Cells
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The human colorectal adenocarcinoma cell line HCT116p53+/+ (WT) were obtained from ATCC (LGC Prochem, Middlesex, UK). HCT116p53-/- Cells were purchased from Horizon Discovery, Cambridge, UK. Cells were cultured in RPM1640 medium supplemented with 10 % Fetal Bovine Serum (FBS), antibiotics penicillin (100 units/mL) and streptomycin (100 μg/mL), and 2 mM L-glutamine (GIBCO, Life technologies). Cells were incubated at 37 °C in a humidified atmosphere with 5% CO2. Cells at passages between 3–8 were used for the study.
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