Telefactor f e2 12

Mice were anaesthetized with ketamine (10%v/v) and xylasine (5% v/v) administered at the rate of 0.1 ml/10 g body mass. Animals were placed on a heated mat inside a sound attenuated chamber. Electrodes (Grass Telefactor F-E2-12) were placed subdermally over the vertex (active), right mastoid (reference), and left mastoid (ground). ABR responses were collected, amplified and averaged using TDT System III (Tucker Davies Technology, Alachua, FL, USA) in conjunction with SigCal and SigGen (Biosig) software. Click stimuli consisted of a 0.1 ms broadband click of alternating polarity. Tone-burst stimuli totalled 7-ms duration, including 1 ms rise/fall time; frequencies used were 8, 16 and 32 kHz. Recordings were performed beginning at 95 dB SPL and decreased in 5 dB increments. ABR thresholds were defined as the lowest dB SPL level at which an ABR trace pattern could be clearly distinguished. Animals were recovered using anaesthetic reversal agent Antipamezole (Antisedan, 0.1 ml 1% v/v). For the analysis of the trombone model the number of mice tested for each genotype at 2-, 6-, 9- and 12 months of age were: Slc4a10^+/+n=18, 13, 6, 7; Slc4a10^+/trmbn=54, 21, 25, 13; and Slc4a10^trmb/trmbn=44, 10, 26, 17, respectively.

Mice were anaesthetized with Ketamine (10%v/v) and Xylasine (5% v/v) administered at the rate of 0.1 ml/10 grams body mass. Animals were placed on a heated mat inside a sound attenuated chamber. Electrodes (Grass Telefactor F-E2–12) were placed subdermally over the vertex (active), right mastoid (reference), and left mastoid (ground). ABR responses were collected, amplified and averaged using TDT System III (Tucker Davies Technology, Alachua, FL, USA) in conjunction with SigCal and SigGen (Biosig) software as previously described^{25 (link)}. Male and female mice were tested at 7 weeks of age.