A chemiluminescent assay was used to measure reactive oxygen species present in the small intestine mucosal homogenates. Chemiluminescence was detected in an H2O2/OH−-luminol-microperoxidase system, using a Lumat LB 9051 luminometer (Berthold Technologies GmbH, Bad Wildbad, Germany) [25 (link)]. Light intensity, given in relative light unit percent of the background (RLU%), was proportional to the concentration of free radical compounds in the sample. luminol, microperoxidase, and hydrogen peroxide were obtained from Sigma-Aldrich Inc. (St. Louis, MO, USA); other chemical reagents were purchased from Reanal Chemical Co. (Budapest, Hungary).
Microperoxidase
Manufactured by Merck Group
Sourced in United States, Sao Tome and Principe
Microperoxidase is a heme-containing enzyme that catalyzes the decomposition of hydrogen peroxide. It is derived from the enzyme horseradish peroxidase and is commonly used in various bioanalytical and biotechnological applications.
Automatically generated - may contain errors
Lab products found in correlation
Luminol, by Merck Group (3 mentions)
Hydrogen peroxide, by Merck Group (2 mentions)
Luminol, by Berthold Technologies (1 mentions)
Hydrochloric acid (hcl), by Reanal (1 mentions)
1 1 diphenyl 2 picryl hydrazil, by Merck Group (1 mentions)
Acetic acid, by Reanal (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
Thiobarbituric acid, by Merck Group (1 mentions)
2 4 6 tripyridyl s triazine, by Merck Group (1 mentions)
Malondialdehyde, by Merck Group (1 mentions)
Nitric acid, by Reanal (1 mentions)
1 1 diphenyl 2 picrylhydrazyl, by Merck Group (1 mentions)
3 protocols using microperoxidase
1
Chemiluminescent Assay for Intestinal Oxidative Stress
2
Metronidazole Biomedical Assay Protocol
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Metronidazole of European Pharmacopoeia quality was provided by Aarti Drugs Limited (Mumbai, India). Ferrous sulphate heptahydrate, acetic acid, hydrochloric acid, sodium acetate and perchloric acid of analytical grade was purchased from Reanal (Budapest, Hungary). 2,4,6-tripyridyl-S-triazine (TPTZ), ascorbic acid, 1,1-diphenyl-2-picrylhydrazil (DPPH), hydrogen peroxide, luminol, microperoxidase and thiobarbituric acid (TBA) were obtained from Sigma-Aldrich Ltd. We purchased malondialdehyde (MDA) in the form of diethyl acetate from Merck Ltd.
3
Vitamin D3 Quantification Protocol
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Vitamin D 3 was a general consumer product on sale in the pharmacy. Each gelatine capsule contained 3000-3300 IU (75-82.5 μg) cholecalciferol (vitamin D 3 ). Standard solutions, nitric acid, and hydrogen chloride were purchased from Reanal (Budapest). 1,1-diphenyl-2-picrylhydrazyl, stable radical, hydrogen peroxide, luminol, microperoxidase were obtained from Sigma (St. Luis). Spectro multi-element standard solutions were used for ICP (CPAchem; Stara Zagora, Bulgaria). The CHR hemoglobin reagent solution was purchased from Reagents Ltd., Hungary.
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