Human galectin 1 quantikine elisa kit

Manufactured by R&D Systems

Sourced in United States

The Human Galectin-1 Quantikine ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed to measure human galectin-1 levels in cell culture supernates, serum, and plasma.

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Lab products found in correlation

Cobas instrument, by Roche (1 mentions) Mouse elisa kit, by BioLegend (1 mentions) Prostaglandin e2 elisa kit, by Abcam (1 mentions) Anti gal1 antibody, by Santa Cruz Biotechnology (1 mentions) Pvdf membrane, by Merck Group (1 mentions)

7 protocols using human galectin 1 quantikine elisa kit

Quantifying Galectin-1 and Cytokines

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Galectin-1 was measured using the Human Galectin-1 Quantikine ELISA Kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturers' instructions. Intra-assay and inter-assay coefficient of variation were 7.1% and 9.5%, respectively. Cytokines were measured in serum using the ultrasensitive multiplex electrochemiluminescence immunoassay (ELISA) (Meso Scale Diagnostics (MSD), Rockville, MD, USA). The human V-PLEX Pro-Inflammatory Panel 1 Human Kit (#K15049) was used to quantify interferon gamma (IFN-γ), interleukin (IL)-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13 and tumor necrosis factor-alpha (TNF-α) according to the manufacturer's instructions.
Routine analyses of glucose, insulin, and cholesterol were performed at the Department of Clinical Chemistry, Sahlgrenska University Hospital, on a Cobas instrument (Roche Molecular Diagnostics). The homeostasis model assessment for insulin resistance (HOMA-IR) was calculated using the formula [glucose]*[insulin]/22.5 [7 (link)].

Fryk E., Strindberg L., Lundqvist A., Sandstedt M., Bergfeldt L., Mattsson Hultén L., Bergström G, & Jansson P.A. (2019). Galectin-1 is inversely associated with type 2 diabetes independently of obesity – A SCAPIS pilot study. Metabolism Open, 4, 100017.

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Cytokine Profiling in Mouse Serum and Cell Culture

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Protein levels of IL-1β, IL-6, and TNF-α in mouse serum were detected using the corresponding mouse ELISA kit according to the manufacturer’s instructions (BioLegend, San Diego, CA, USA; http://www.biolegend.com/). The protein level of Galectin-1 in the cell culture supernatants was detected using Human Galectin-1 Quantikine ELISA kit (R&D Systems, Catalog # DGAL10; https://www.bio-techne.com).

Li J., Pan Y., Yang J., Wang J., Jiang Q., Dou H, & Hou Y. (2022). Tumor necrosis factor-α-primed mesenchymal stem cell-derived exosomes promote M2 macrophage polarization via Galectin-1 and modify intrauterine adhesion on a novel murine model. Frontiers in Immunology, 13, 945234.

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Galectin-1 Serum Levels in CLL Patients

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In the experiment, sera of healthy donors and CLL patients with clinically manifested immunodeficiency were used. Previously, Gal-1 serum level was determined using the Human Galectin-1 Quantikine ELISA kit (R&D Systems, Minneapolis, Minnesota, USA) according to the instructions provided. The demographic characteristics of the study participants are given in Table 1.

Kostic M., Dzopalic T., Marjanovic G., Urosevic I, & Milosevic I. (2021). Immunomodulatory effects of galectin-1 in patients with chronic lymphocytic leukemia. Central-European Journal of Immunology, 46(1), 54-62.

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Soluble Galectin-1 Levels in Metastatic Melanoma

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Before patient enrollment, the Bioethical Committee issued a positive decision about this pilot study, and the signed informed consent from all patients was obtained. Nine enrolled patients had been diagnosed with metastatic melanoma with BRAF mutation. All patients received a combination of BRAF + MEK inhibitors, no grade 3–5 toxicity acc. CTCEA were observed during treatment. The patients’ sera were collected before the first administration of BRAF + MEK inhibitors and every 1–6 months from initiation of treatment at the time of radiological and clinical assessment. Radiological assessment was done according to RECIST 1.1. Patients’ characteristics and therapy response status are presented in Table S3. Soluble Gal‐1 in patients’ plasma was quantified using a commercial ELISA kit (Human Galectin‐1 Quantikine ELISA Kit; R&D systems, Minneapolis, MN, USA), according to the manufacturer’s protocol. Serum was diluted ten times, and all samples were analyzed in duplicates.

Górniak P., Wasylecka‐Juszczyńska M., Ługowska I., Rutkowski P., Polak A., Szydłowski M, & Juszczyński P. (2020). BRAF inhibition curtails IFN‐gamma‐inducible PD‐L1 expression and upregulates the immunoregulatory protein galectin‐1 in melanoma cells. Molecular Oncology, 14(8), 1817-1832.

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Quantification of Galectin-1 and PGE2

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The gal-1 level in serum (10-fold dilution) or culture media (2-fold dilution) were determined by Human Galectin-1 Quantikine ELISA Kit (R&D Systems), PGE2 concentration in cell culture media was determined by Prostaglandin E2 ELISA Kit (abcam) according to the protocol recommended by manufacturers.

Zhou X., Li D., Wang X., Zhang B., Zhu H, & Zhao J. (2014). Galectin-1 is overexpressed in CD133+ human lung adenocarcinoma cells and promotes their growth and invasiveness. Oncotarget, 6(5), 3111-3122.

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Quantification of Galectin-1 in BALF

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Proteins in the BALFs were separated on SDS-PAGE gels (4– 15%; Bio-Rad Laboratories, Hercules, CA, USA) and transferred to PVDF membranes (Millipore, Bedford, MA, USA). Membranes were blocked with 5% milk in TBS and blotted with anti-Gal1 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA), followed by HRP-conjugated anti-mouse IgG (Pierce, Rockford, IL, USA). Blots were developed with SuperSignal West Pico chemiluminescent substrate (Pierce). The concentration of galectin-1 in BALFs was also determined with Human Galectin-1 Quantikine ELISA Kit (R&D Systems) according to the manufacturer’s recommendation.

Bekkers J.M., Forster I.C, & Greeff N.G. (1990). Gating current associated with inactivated states of the squid axon gating channel.. Proceedings of the National Academy of Sciences of the United States of America, 87(21), 8311-8315.

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Quantifying Serum Galectin-1 Levels

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Galectin-1 was measured in human sera sampled at the study baseline with the Human Galectin-1 Quantikine ELISA Kit (R&D Systems, MN, USA) according to the manufacturer’s instructions. Intra-assay and inter-assay coefficients of variation were 7.1% and 9.5%, respectively.

Drake I., Fryk E., Strindberg L., Lundqvist A., Rosengren A.H., Groop L., Ahlqvist E., Borén J., Orho-Melander M, & Jansson P.A. (2021). The role of circulating galectin-1 in type 2 diabetes and chronic kidney disease: evidence from cross-sectional, longitudinal and Mendelian randomisation analyses. Diabetologia, 65(1), 128-139.

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