D5100 digital camera

Manufactured by Nikon

Sourced in Japan

The Nikon D5100 is a 16.2-megapixel digital single-lens reflex (DSLR) camera. It features a 3-inch vari-angle LCD monitor, 1080p full HD video recording, and an EXPEED 2 image-processing engine.

Automatically generated - may contain errors

Lab products found in correlation

Af s micro nikkor 60 mm f 2.8 g ed lens, by Nikon (3 mentions) Shandon finesse, by Thermo Fisher Scientific (2 mentions) Oil red o solution, by Merck Group (1 mentions)

Close spelling variants of this product

D5100 camera D5100 Digital camera

6 protocols using d5100 digital camera

Evaluating Wound Healing in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

The clinical condition of mice (e.g., total wound area, progress of healing process, skin inflammation intensity and mobility) was recorded daily. The animals were weighted periodically. Skin images for photo-documentation were acquired using a Nikon D5100 digital camera (Nikon, Tokyo, Japan) equipped with an AF-S Micro Nikkor 60 mm f/2.8 G ED lens (Nikon, Tokyo, Japan), which was at a fixed distance of 30 cm perpendicular to the subject. The photographs were digitized, and the wound area was measured using Adobe Photoshop C5. Wound closure was defined as a reduction in the wound area and the results were expressed as a percentage (%) of the original wound area.
At the end of the experiment, when total wound closure could be observed for at least one group of treated mice, the mice were sacrificed and skin tissue was excised. Skin sections of specimens from all groups were performed using a paraffin microtome (Shandon Finesse, Thermo Fisher Scientific, Cheshire, UK) and stained with hematoxylin and eosin stain kit (Atom Scientific, Cheshire, UK). Parameters such as inflammation, hyperkeratosis, parakeratosis and skin structure, were estimated.

Sofrona E., Tziveleka L.A., Harizani M., Koroli P., Sfiniadakis I., Roussis V., Rallis M, & Ioannou E. (2020). In Vivo Evaluation of the Wound Healing Activity of Extracts and Bioactive Constituents of the Marine Isopod Ceratothoa oestroides. Marine Drugs, 18(4), 219.

+ Open protocol

+ Expand

Imaging and Evaluating Wound Area

Check if the same lab product or an alternative is used in the 5 most similar protocols

Wounds were photographed after wound induction and every other day until the end of the experiments using a Nikon D5100 digital camera (Nikon, Tokyo, Japan) equipped with an AF-S Micro Nikkor 60 mm f/2.8 G ED lens (Nikon, Tokyo, Japan), which was fixed at a distance of 20 cm perpendicular to the subject. Pictures were also acquired using an Antera 3D camera (Miravex, Dublin, Ireland). Antera 3D uses an optical method combined with a complex algorithm to capture images in three dimensions. Its software, version 2.11.5, was used to evaluate wound area (mm²) and volume [28 (link)].

Vitsos A., Ieronymaki D., Kostaki M., Almpani C., Barda C., Kikionis S., Sfiniadakis I., Dallas P, & Rallis M.C. (2024). In Vivo Evaluation of Wound Healing Efficacy of Gel-Based Dressings Loaded with Pycnogenol™ and Ceratothoa oestroides Extracts. Gels, 10(4), 233.

+ Open protocol

+ Expand

Murine Skin Inflammation Assessment

Check if the same lab product or an alternative is used in the 5 most similar protocols

The clinical condition of mice (e.g., skin inflammation intensity and mobility) was recorded daily. The animals were weighted at day 1, 4, 7 and 10. Skin images for photo-documentation were acquired using a Nikon D5100 digital camera (Nikon, Tokyo, Japan) equipped with a AF-S Micro Nikkor 60 mm f/2.8 G ED lens (Nikon, Tokyo, Japan), which was at a fixed distance of 33 cm perpendicular to the subject.
At the end of the experiment, which lasted for 10 days when no inflammation could be observed for at least one group of treated mice, the mice were sacrificed and skin tissue was excised. Skin sections of specimens from all groups were performed using a paraffin microtome (Shandon Finesse, Thermo Scientific, USA) and stained with haematoxylin & eosin stain kit (Atom Scientific, Cheshire, UK). Parameters such as inflammation, hyperkeratosis, parakeratosis and skin structure, were estimated.

Kotroni E., Simirioti E., Kikionis S., Sfiniadakis I., Siamidi A., Karalis V., Vitsos A., Vlachou M., Ioannou E., Roussis V, & Rallis M. (2019). In Vivo Evaluation of the Anti-Inflammatory Activity of Electrospun Micro/Nanofibrous Patches Loaded with Pinus halepensis Bark Extract on Hairless Mice Skin. Materials, 12(16), 2596.

+ Open protocol

+ Expand

Diapausing Beetle Ovary Activation by 20E

Check if the same lab product or an alternative is used in the 5 most similar protocols

For the 20E induction, the original 20E (Selleck Chemicals, Houston, TX, USA) was dissolved in dimethyl sulfoxide (DMSO) to a concentration of 50 μg/μL. Then the solution was separately diluted to 2.5, 5, 10 and 25 μg/μL concentrations using 1× phosphate buffer saline (PBS) (HyClone, Logan, Utah, USA). Using a micro-injector (Nanolater2010, WPI), 200 nL of each 20E dilution was injected into diapause female beetles at 0 days PE (newly emerged without feeding) to reach the final 20E contents of 0.5, 1, 2 and 5 μg. Meanwhile, an equal volume of DMSO and 1× PBS was injected as the solvent control. We finally chose a concentration of 1 μg for the following experiments due to its favorable induction effect and low mortality (S9 Fig). To analyze the 20E-regulated phenotype, the images of the ovaries were collected using a stereo microscope fitted with a Nikon D5100 digital camera (Nikon, Tokyo, Japan), and the ovary size was determined by measuring the length and width with ScopePhoto 3.0 (Scopetek Opto Electric, Hangzhou, China) at 4 days PE. In addition, we graded the development of the ovaries to facilitate the statistical analysis of ovarian phenotypes (S10 Fig).

Guo S., Tian Z., Wu Q.W., King-Jones K., Liu W., Zhu F, & Wang X.P. (2021). Steroid hormone ecdysone deficiency stimulates preparation for photoperiodic reproductive diapause. PLoS Genetics, 17(2), e1009352.

+ Open protocol

+ Expand

Lipid Staining Protocol with Oil Red O

Check if the same lab product or an alternative is used in the 5 most similar protocols

Each group sample was fixed in 4% formalin for 5 min. 0.5% Oil red O solution (sigma) was prepared in isopropanol and diluted 3:2 (v:v) with deionized water. Each sample was incubated with 1 mL Oil red O for 15 min at room temperature. After rinsed 3 times with PBS, samples were visualized under D5100 Digital Camera (Nikon).

Tang Y., Xu Y., Xiao Z., Zhao Y., Li J., Han S., Chen L., Dai B., Wang L., Chen B, & Wang H. (2017). The combination of three-dimensional and rotary cell culture system promotes the proliferation and maintains the differentiation potential of rat BMSCs. Scientific Reports, 7, 192.

+ Open protocol

+ Expand

Silver Nitrate Staining of Calcium Deposition

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cells were washed twice with PBS and fixed in 4% paraformaldehyde for 30 min and then rinsed with deionized water. After a brief air dry, the samples were exposed to ultraviolet light in 1% aqueous silver nitrate under UV exposure for 30 min. Calcium deposition was appeared as black spots, and then the samples were rinsed fully with distilled water and 5% sodium thiosulfate to fix the positive dark staining and remove excess silver nitrate. Then the samples were visualized under D5100 Digital Camera (Nikon).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!