Pi 4 5 p2

Manufactured by Cayman Chemical

Sourced in Germany

PI(4,5)P2 is a phosphoinositide that serves as a key signaling molecule in cellular processes. It plays a crucial role in regulating various cellular functions, including membrane trafficking, signal transduction, and cytoskeletal organization. PI(4,5)P2 is a common substrate for numerous cellular enzymes and is involved in the generation of other important signaling molecules.

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Lab products found in correlation

Pi3 kinase activity elisa kit, by Echelon Biosciences (1 mentions)

2 protocols using pi 4 5 p2

OCRL1 and RhoA Binding Assay

Cited 1 time

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A binding assay of OCRL1 and RhoA to liposomes containing PI(4,5)P₂ was carried out using the method described previously^{27 (link)}, with modifications, using PI(4,5)P₂ (Cayman Chemical) and/or phosphatidylcholine (PC; Sigma-Aldrich). Cell lysates were obtained from cells transiently expressing Halo-OCRL1 and/or Halo-RhoA. Recombinant EGFP-PRIP1 and GST-PRIP2 were purified from MCF-7 cells stably expressing EGFP-Prip1 and HEK293 cells transiently expressed GST-PRIP2. The detailed method is described in Fig. 7a,c.

Asano S., Ikura Y., Nishimoto M., Yamawaki Y., Hamao K., Kamijo K., Hirata M, & Kanematsu T. (2019). Phospholipase C-related catalytically inactive protein regulates cytokinesis by protecting phosphatidylinositol 4,5-bisphosphate from metabolism in the cleavage furrow. Scientific Reports, 9, 12729.

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PI3K Binding Assay with PRIP1

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A binding assay of PI3K to liposomes containing with PI(4,5)P₂ was carried out following previously described methods^{15 (link)}, with modifications, using recombinant His-tagged PI3Kα (10 pg; Jena Bioscience, Jena, Germany) and PI(4,5)P₂ (Cayman, Ann Arbor, MI, USA) and/or PC (Sigma-Aldrich, St. Louis, MO, USA) in the presence or absence of purified recombinant GST-tagged PRIP1 PHL. PI3K activity was measured in vitro using the PI3-Kinase Activity ELISA Kit (Echelon).

Asano S., Taniguchi Y., Yamawaki Y., Gao J., Harada K., Takeuchi H., Hirata M, & Kanematsu T. (2017). Suppression of cell migration by phospholipase C-related catalytically inactive protein-dependent modulation of PI3K signalling. Scientific Reports, 7, 5408.

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