K2edta tubes

Manufactured by BD

Sourced in United States, United Kingdom

K2EDTA tubes are laboratory specimen collection tubes that contain the anticoagulant dipotassium ethylenediaminetetraacetic acid (K2EDTA). The primary function of these tubes is to prevent blood from clotting during the collection and transportation process, allowing for accurate analysis of cellular components and other blood-based tests.

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Close spelling variants of this product

K2EDTA (197 citations) BD Vacutainer K2EDTA tubes (70 citations) BD Vacutainer® spray-coated K2EDTA tubes (19 citations) K2EDTA Microtainer tubes (15 citations) K2EDTA-coated tubes (14 citations) BD Vacutainer® Plus Plastic K2EDTA Tubes (13 citations) K2EDTA coated microtainer tubes (12 citations) K2EDTA-coated vacutainer tubes (9 citations) K2EDTA anticoagulant tubes (7 citations) K2EDTA vacutainer blood collection tubes (5 citations)

66 protocols using k2edta tubes

Detailed Plasma Biochemical Analysis Protocol

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Blood samples were collected via the jugular catheter (0600 h) on d 3 to 5 of P1 and daily during P2 and P3 for complete blood count (CBC) analysis. Blood samples were also collected on d 5 of P1, d 2 of P2, and d 1 to 3 of P3 for plasma analysis (24, 48, and 72 h relative to the first abomasal infusion). Plasma samples were collected in K 2 EDTA tubes (Becton Dickinson Co.) and were subsequently centrifuged at 1,500 × g for 15 min at 4°C before being aliquoted into microcentrifuge tubes for storage at -20°C until analysis. For CBC data, a 3-mL blood sample was collected in K 2 EDTA tubes (Becton Dickinson Co.) and stored at 4°C for ~4 h before submitting to the Iowa State University's Department of Veterinary Pathology for analysis.
Plasma insulin, nonesterified fatty acids (NEFA), BHB, BUN, glucose, LPS-binding protein (LBP), and serum amyloid A (SAA) concentrations were determined using commercially available kits according to manufacturers' instructions (insulin, Mercodia AB; NEFA, Wako Chemicals USA; BHB, Pointe Scientific Inc.; BUN, Teco Diagnostics; glucose, Wako Chemicals USA Inc.; LBP, Hycult Biotech; SAA, Tridelta Development Ltd.). The inter-and intraassay coefficients of variation for insulin, NEFA, BHB, BUN, glucose, LBP, and SAA were 10.4 and 6.1%, 9.1 and 4.0%, 11.8 and 5.1%, 11.1 and 5.4%, 12.1 and 5.0%, 15.3 and 3.8%, and 16.2 and 4.2%, respectively.

Abeyta M.A., Horst E.A., Goetz B.M., Mayorga E.J., Rodriguez-Jimenez S., Caratzu M, & Baumgard L.H. (2023). Effects of hindgut acidosis on production, metabolism, and inflammatory biomarkers in previously immune-activated lactating dairy cows. Journal of dairy science, 106(6).

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Fasting Blood Sample Collection

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Participants were instructed to avoid consumption of alcohol and doing vigorous physical activity the day before blood sampling. Venous blood samples were drawn after an overnight fast (≥10 h). Serum was obtained from silica gel tubes (Becton, Dickinson and Company, Franklin Lakes, NJ, USA) and kept at room temperature for >30-60< min, until centrifugation (1500 × g, 15 min). Plasma was obtained from K2EDTA tubes (Becton, Dickinson and Company, Franklin Lakes, NJ, USA), immediately placed on ice, and centrifuged within 10 min (2000 × g, 4°C, 15 min). Lithium-Heparin tubes (Becton, Dickinson and Company, Franklin Lakes, NJ, USA) and K2EDTA tubes with whole blood were kept at room temperature. Serum and plasma concentrations of fasting glucose, insulin, HbA1c, TG, total-cholesterol, LDL-C, HDL-C, hsCRP, creatinine, eGFR, ASAT, ALAT, Gamma-GT and mercury, and glucose and insulin after a 2h-OGTT, were measured by standard methods at an accredited routine laboratory (Fürst Medical Laboratory, Oslo, Norway).

Hustad K.S., Ottestad I., Hjorth M., Dalen K.T., Sæther T., Sheikh N.A., Strømnes M., Ulven S.M, & Holven K.B. (2021). No effect of salmon fish protein on 2-h glucose in adults with increased risk of type 2 diabetes: a randomised controlled trial. The British journal of nutrition, 126(9).

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Blood Collection for Toxicity Analysis

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After CO₂ asphyxiation, blood was collected by cardiac puncture from paired
animals in K2-EDTA tubes (cat # 365973, Becton, Dickenson and Co., Franklin Lakes, NJ,
USA). Plasma was separated from leukocytes by centrifugation (4°C, 4,250 × g), snap frozen
in liquid nitrogen, and stored at −20°C. Samples were shipped frozen for expanded toxicity
analysis (cat # 60514, IDEXX BioResearch, Columbia, MO, USA).

Leo V.I., Bunte R.M, & Reilly P.T. (2015). BALB/c-congenic ANP32B-deficient mice reveal a modifying locus that determines viability. Experimental Animals, 65(1), 53-62.

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Vancomycin HCl Purification and Preparation

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Vancomycin HCl was purchased from Cayman Chemicals (Ann Arbor, MI, USA). Deionized water was prepared using a Milli-Q® Advantage A10® purification system from Merck Millipore (Darmstadt, Germany). Drug-free human serum was obtained from the Hemostasis Laboratory of Erasmus MC, University Medical Centre (Rotterdam, Netherlands). Becton Dickinson K₂EDTA tubes and LiHep syringes were used for blood collection (Franklin Lakes, NJ, USA).

Smeets T.J., van de Velde D., Koch B.C., Endeman H, & Hunfeld N.G. (2022). Using Residual Blood from the Arterial Blood Gas Test to Perform Therapeutic Drug Monitoring of Vancomycin: An Example of Good Clinical Practice Moving towards a Sustainable Intensive Care Unit. Critical Care Research and Practice, 2022, 9107591.

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Flow Cytometric Analysis of ALS Patients

Cited 3 times

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Peripheral blood samples from 50 age and gender matched healthy volunteers and 80 ALS patients were collected in K₂EDTA tubes (BectonDickinson) at initial and return visits as indicated. Un-manipulated whole blood was stained directly with antibodies. For a subset of initial patients (n = 17) flow cytometry was performed on the 2 laser, 4-color FACSCalibur (BectonDickinson) with antibody panels previously described[13 (link), 17 (link)]. For the remaining 63 patients, flow cytometry was performed with expanded flow protocols on the 3 laser, 10-color Gallios Flow Cytometer (Beckman Coulter). All 10-color procedures, antibodies, flow protocols, instrument settings, and gating strategies have been previously described by Gustafson et al[14 (link)]. Some patients were additionally stained with a modified T cell signaling protocol[18 (link)] to assess CD28 expression on CD3⁺CD56⁺ T cells. Analysis of flow data was performed using Cellquest (BD) or Kaluza (Beckman Coulter) software.

Gustafson M.P., Staff N.P., Bornschlegl S., Butler G.W., Maas M.L., Kazamel M., Zubair A., Gastineau D.A., Windebank A.J, & Dietz A.B. (2017). Comprehensive immune profiling reveals substantial immune system alterations in a subset of patients with amyotrophic lateral sclerosis. PLoS ONE, 12(7), e0182002.

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Isolation of Exosomes from Rat Plasma

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Blood samples were collected in K₂-EDTA tubes (Becton Dickinson) immediately after euthanasia and processed for plasma preparation within 5 min. The samples were first centrifuged at 1500 × g at 4 °C for 15 min. And the supernatants were carefully collected without disturbing the lower layers that contained platelets, and then transferred to nuclease-free tubes. Rat blood samples were collected at pre- (baseline) and post RIPC^{35 (link)}. Exosomes from sham-operated animals served as control exosomes. The exosomes were isolated from plasma using ExoQuick precipitation solution^{36 (link)} (SBI, USA) according to the manufacturer’s instructions. Briefly, plasma was filtered by using a 0.22-μm pore filter and treated with thrombin to pre-clear fibrin and prevent its co-precipitation using Thrombin plasma prep for exosome precipitation (500 µl at 500 U/ml) as previously described^{37 (link)}. Cell-free plasma samples were mixed with ExoQuick precipitation solution. After the mixtures were incubated for 30 min at 4 °C and centrifuged at 1500 × g for 30 min at 4 °C, the obtained pellets were washed with PBS, after which the exosome pellets were suspended in PBS and stored at −80 °C until use.

Minghua W., Zhijian G., Chahua H., Qiang L., Minxuan X., luqiao W., Weifang Z., Peng L., Biming Z., Lingling Y., Zhenzhen W., Jianqing X., Huihui B., Xiaozhong W, & Xiaoshu C. (2018). Plasma exosomes induced by remote ischaemic preconditioning attenuate myocardial ischaemia/reperfusion injury by transferring miR-24. Cell Death & Disease, 9(3), 320.

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Equine Bronchoalveolar Lavage Procedure

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Blood was collected from the jugular vein into K₂EDTA tubes (Becton, Dickson, and Co., Franklin Lakes, NJ) prior to sedation, and was submitted to the institution's clinical pathology laboratory for CBC determination using an Advia 2120i hematology instrument (Siemens Healthcare Diagnostics, Inc., Tarrytown, NY) and heat-precipitated fibrinogen measurement using an analog Goldberg TS meter clinical refractometer (Reichert Technologies, Buffalo, NY) by a licensed medical technologist.
Bronchoalveolar lavage procedure was performed on standing, sedated horses using a blind technique with a BAL catheter (Mila International, Florence, KY). Sedation for each horse was determined by the attending clinician and consisted of xylazine (0.2–0.5 mg/kg) or detomidine (5–10 μg/kg), combined with butorphanol (10–20 μg/kg) IV. The BAL catheter was passed nasotracheally until wedged. Once wedged, the cuff was inflated and 200 mL of sterile saline was infused and re-aspirated by 60 ml syringe. The first 10 ml of aspirated sample was discarded as dead space, with subsequent BALF collected and pooled for analysis.

Woodrow J.S., Hines M., Sommardahl C., Flatland B., Lo Y., Wang Z., Sheats M.K, & Lennon E.M. (2023). Initial investigation of molecular phenotypes of airway mast cells and cytokine profiles in equine asthma. Frontiers in Veterinary Science, 9, 997139.

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Hematological Profiles of Cancer Patients

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This study comprised a total of 1003 blood samples from the daily laboratory workload of the Tianjin Medical University Cancer Institute and Hospital. The samples were collected in K₂‐EDTA tubes (Becton Dickinson) and were tested using the XN‐9000 system within 30 minutes to 2 hours after collection. The automated results obtained by analysis modules were inputted to Laboman 6.0 software. For preparing the smear, the speed and angle of the slide were adjusted automatically using SP‐10, according to the hematocrit (HCT) value of each sample, and then, two smears were pushed and stained. Each smear was pre‐examined using DI60. The samples were collected from more than 20 clinical in‐patient departments, including hematology, breast, colorectal, lymphoma, pediatrics, and lung oncology from February 2017 to November 2017. Among the included patients, 458 were males and 545 females, aged 1‐90 years, including first‐time and re‐visiting patients. Another 215 samples from the daily laboratory workload were used for validation.
This study was performed in accordance with the Declaration of Helsinki and was approved by the ethics committee of Tianjin Medical University Cancer Institute and Hospital. Written informed consent was obtained from the participants.

Wang X., Wang X., Ge P., Zhao X., Chen C., Hu S, & Ren L. (2020). Establishment of improved review criteria for hematology analyzers in cancer hospitals. Journal of Clinical Laboratory Analysis, 35(2), e23638.

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Venipuncture and Sample Processing Protocol

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Samples are drawn by routine venipuncture using the order of blood draw as suggested by the Clinical and Laboratory Standards Institute (12 ).
In this study, Na-citrate vacutainer tubes for coagulation tests and ESR, tubes with gel separator for clinical chemistry and immunoassay tests and K₂EDTA tubes for hematology and HbA1c (Becton Dickinson and Company, USA) were used. Then, the specimens were transferred to the laboratory by the trained staff for processing. At the time of sample receipt, technicians visually checked the samples with regard to volume, label, clot and simultaneously matched label with those on the accompanying requisition form and accepted accordingly.
Any inappropriateness was recorded in laboratory information system. The specimens were allowed to clot, centrifuged at 1500 × g for 10 minutes and then delivered to the analyzers.

Atay A., Demir L., Cuhadar S., Saglam G., Unal H., Aksun S., Arslan B., Ozkan A, & Sutcu R. (2014). Clinical biochemistry laboratory rejection rates due to various types of preanalytical errors. Biochemia Medica, 24(3), 376-382.

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Profiling Severe COVID-19 Immune Responses

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We prospectively evaluated patients diagnosed with severe COVID-19 acute infection that were admitted to Hospital Moinhos de Vento and a sample of uninfected healthy individuals. The diagnosis of COVID-19 was confirmed by SARS-CoV-2 nucleic acid testing of nasal and pharyngeal throat swab specimens using real-time RT-PCR assay and the severity of COVID-19 was classified according to the WHO interim guidance [15] . This study was approved by the Moinhos de Vento Ethics Committee N 3,977,144 (Porto Alegre/Brazil) and informed consent was obtained from all participants.
Blood samples (5 mL) were obtained from controls and severe COVID-19 patients into K2EDTA tubes (Becton & Dickinson, USA) within 6 h from hospital admission.
Concomitantly, nasopharyngeal samples were obtained from the participants according to the CDC recommendations [16] using FLOQSwab® with eNAT medium (COPAN Diagnostics Inc.).
Flow cytometry experiments (described below) were conducted with fresh blood. The remaining blood was centrifuged (1500 g, 10 min), plasma was aliquoted and kept at −80 °C until plasma analysis.

Romão P.R., Teixeira P.C., Schipper L., da Silva I., Santana Filho P., Júnior L.C., Peres A., Gonçalves da Fonseca S., Chagas Monteiro M., Lira F.S., Andrey Cipriani Frade M., Comerlato J., Comerlato C., Sant'Anna F.H., Bessel M., Abreu C.M., Wendland E.M, & Dorneles G.P. (2022). Viral load is associated with mitochondrial dysfunction and altered monocyte phenotype in acute severe SARS-CoV-2 infection. International Immunopharmacology, 108, 108697.

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