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Etga 200

Manufactured by BioAssay Systems
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Sourced in United States

The ETGA-200 is a laboratory instrument designed for the enzymatic determination of total glutathione (GSSG + GSH) in biological samples. It utilizes a colorimetric method to quantify the total glutathione levels in the sample.

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Lab products found in correlation

Mf2100, by BioAssay Systems (2 mentions) Diua 250, by R&D Systems (2 mentions) Microtainer tube, by BD (2 mentions) Eastr 100, by BioAssay Systems (2 mentions) Diua 250, by BioAssay Systems (2 mentions) Ealt 100, by Crystal Chem (2 mentions) Mob00, by R&D Systems (2 mentions) Mf2100, by R&D Systems (2 mentions) Ab65390, by Abcam (2 mentions) Ehdl 100, by BioAssay Systems (1 mentions) Mouse glucose assay kit, by Crystal Chem (1 mentions) Egly 200, by BioAssay Systems (1 mentions) E2hl 100, by BioAssay Systems (1 mentions) Microvette cb 300 lh tubes, by Sarstedt (1 mentions) Fuji dri chem slide, by Fujifilm (1 mentions) Csb e08118m, by Cusabio (1 mentions) Ab6533, by Abcam (1 mentions) Ab108785, by Abcam (1 mentions) Ab65341, by Abcam (1 mentions)

8 protocols using etga 200

1

Serum and Tissue Biochemical Analysis in Mice

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Blood was collected in microtainer tubes (BD) from cardiac puncture of mice under isoflurane, and serum was obtained after centrifugation at 13000 rpm for 2 min at room temperature. Serum parameters was performed biochemically following manufacturer’s instruction (uric acid: Bioassay systems, DIUA-250; FGF21: R&D, MF2100, AST: Bioassay Systems, EASTR-100, ALT: Bioassay Systems, EALT-100, Insulin: Crystal Chem, 90080, Leptin: R&D, MOB00, FGF21: R&D, MF2100). Determination of parameters in tissue was performed in freeze-clamped tissues and measured biochemically following manufacturer’s protocol (Triglycerides (Liver): Bioassay Systems, ETGA-200; uric acid: Bioassay Systems DIUA-250).
Andres-Hernando A., Orlicky D.J., Kuwabara M., Ishimoto T., Nakagawa T., Johnson R.J, & Lanaspa M.A. (2020). Deletion of fructokinase in the liver or in the intestine reveals differential effects on sugar-induced metabolic dysfunction. Cell metabolism, 32(1), 117-127.e3.
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2

Plasma Lipid and Glycemic Biomarkers

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Plasma triglycerides (TG) were measured with an enzymatic colorimetric commercial assay kit (ETGA-200 and EGLY-200, BioAssay Systems). Total cholesterol, combined low-density lipoprotein cholesterol (LDL-C), and very low density lipoprotein cholesterol (VLDL-C), as well as high-density lipoprotein cholesterol (HDL-C), were measured using an enzymatic colorimetric commercial assay kit (EHDL-100, BioAssay Systems). In the animals undergoing CEUMI, plasma HbA1c was measured using a sandwich enzyme-linked immunosorbent commercial assay kit (E4657-100, BioVision), and nonfasting plasma glucose was measured using mouse glucose assay kit (#81692, Crystal Chem) in blood drawn after the imaging studies. In a separate group of animals (n = 20 on liraglutide treatment and n = 20 on vehicle treatment), whole-blood HbA1c was measured at baseline, day 44, and day 80 in a 5-μL full blood sample taken from the tip of the tail by puncturing the capillary bed with a lancet, using a heparinized capillary tube to sample the blood. The capillary tube was then shaken into 500 μL of Hitachi Hemolyzing Reagent and measured in a Hitachi 912 autoanalyzer (Roche A/S Diagnostics), according to the manufacturer’s instructions. Assays were performed in duplicates according to manufacturer’s protocol with the exception of whole-blood HbA1c assays, which were done as single measurements.
Punjabi M., Kosareva A., Xu L., Ochoa-Espinosa A., Decembrini S., Hofmann G., Wyttenbach S., Rolin B., Nyberg M, & Kaufmann B.A. (2022). Liraglutide Lowers Endothelial Vascular Cell Adhesion Molecule-1 in Murine Atherosclerosis Independent of Glucose Levels. JACC: Basic to Translational Science, 8(2), 189-200.
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3

Intracellular Lipid Droplet Analysis

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Intracellular lipid droplet analysis was performed using Nile red staining. Cells were treated with 1 mM FFA and fixed with 4% paraformaldehyde for 15 min. After staining with Nile red dye (1 μg/mL), the images were acquired using a fluorescence microscope. TG content in CLL cells was quantified using a triglyceride quantification kit (BioAssay Systems, ETGA-200) according to the manufacturer's instructions.
Wu Z., Gu D., Wang R., Zuo X., Zhu H., Wang L., Lu X., Xia Y., Qin S., Zhang W., Xu W., Fan L., Li J, & Jin H. (2022). CircRIC8B regulates the lipid metabolism of chronic lymphocytic leukemia through miR199b-5p/LPL axis. Experimental Hematology & Oncology, 11, 51.
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4

Plasma Lipid and Glucose Profiling

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After blood was drawn by cardiac puncture, plasma was isolated using Microvette CB 300 LH tubes (Sarstedt). The concentrations of total cholesterol, LDL/VLDL cholesterol, HDL cholesterol and triglycerides were determined using enzymatic colorimetric assays (#E2HL-100 and #ETGA-200; BioAssay Systems) as per the manufacturer's instructions. Glucose levels were detected using the Contour®Next EZ Blood Glucose Monitoring System with Contour®Next test strips.
Colijn S., Muthukumar V., Xie J., Gao S, & Griffin C.T. (2020). Cell-specific and athero-protective roles for RIPK3 in a murine model of atherosclerosis. Disease Models & Mechanisms, 13(1), dmm041962.
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5

Measuring Insulin and Lipid Levels

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The plasma insulin level was determined with an ELISA (Mercodia AB, Uppsala, Sweden). High-density lipoprotein (HDL) and low-density lipoprotein (LDL) cholesterol fractions and triglycerides (TGs) blood concentration were measured with the BioAssay Systems (HDL/LDL assay kit E2H:−100 and TG assay kit ETGA-200, respectively). Homeostasis model assessment-insulin resistance (HOMA-IR) was used to assess β-cell function and IR from basal glucose and insulin according to the formula: fasting glucose (mmol/L) × fasting insulin (µU/mL) / 22.5.3 (link)
Maciak S., Sawicka D., Sadowska A., Prokopiuk S., Buczyńska S., Bartoszewicz M., Niklińska G., Konarzewski M, & Car H. (2020). Low basal metabolic rate as a risk factor for development of insulin resistance and type 2 diabetes. BMJ Open Diabetes Research & Care, 8(1), e001381.
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6

Serum and Tissue Biochemical Analyses in Mice

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Blood was collected in microtainer tubes (BD Biosciences) from cardiac puncture of mice under isoflurane, and serum was obtained after centrifugation at 13,148g for 2 minutes at room temperature. Serum parameters was performed biochemically following manufacturer’s instruction (uric acid: BioAssay Systems, DIUA-250; FGF21: R&D, MF2100; AST: BioAssay Systems, EASTR-100; ALT: BioAssay Systems, EALT-100; insulin: Crystal Chem, 90080; leptin: R&D, MOB00; FGF21: R&D, MF2100). Determination of parameters in tissue was performed in freeze-clamped tissues and measured biochemically following manufacturer’s protocol (triglycerides [liver]: BioAssay Systems, ETGA-200; uric acid: BioAssay Systems, DIUA-250).
Andres-Hernando A., Jensen T.J., Kuwabara M., Orlicky D.J., Cicerchi C., Li N., Roncal-Jimenez C.A., Garcia G.E., Ishimoto T., Maclean P.S., Bjornstad P., Sanchez-Lozada L.G., Kanbay M., Nakagawa T., Johnson R.J, & Lanaspa M.A. (2021). Vasopressin mediates fructose-induced metabolic syndrome by activating the V1b receptor. JCI Insight, 6(1), e140848.
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7

Metabolic Biomarkers in Blood and Liver

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Whole animal or human blood samples were separated by being allowed to sit for 30 min or using EDTA-coated tubes, followed by centrifugation at 4000 ×g for 15 min and storage at -80°C until use. Blood glucose, triglycerides, total cholesterol, glucose, and AST/ALT were measured using Fuji Dri-Chem Slides (Fujifilm). Leptin, ghrelin, GLP-1, adiponectin, and glucagon concentrations were determined in duplicate according to the manufacturer’s ELISA instructions. ELISA kits for mouse adiponectin and leptin were purchased from Abcam (ab108785 and ab100718; Cambridge, UK), and ELISA kits for mouse ghrelin, GLP-1, and glucagon were from Cusabio (CSB-E09817m, CSB-E08118m, and CSB-E15775m, respectively; Houston, TX). Enzyme immunoassay (EIA) kits for human/mouse apelin-13 were purchased from Phoenix Pharmaceutical (EK-057-19, Burlingame, CA). ELISA kits for glucose (Ab6533, Abcam), triglycerides (ETGA-200, Bioassay Systems, Hayward, CA, USA), and cholesterol (ab65390, Abcam) were used to measure levels in the liver tissues and sera saved after the experimental diets and from control and NAFLD patients.
Pinanga Y.D., Lee H.A., Shin E.A., Lee H., Pyo K.H., Kim J.E., Lee E.H., Kim W., Kim S., Kim H.Y, & Lee J.W. (2023). TM4SF5-mediated abnormal food-intake behavior and apelin expression facilitate non-alcoholic fatty liver disease features. iScience, 26(9), 107625.
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8

Liver lipid profile analysis

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Assay kits for free fatty acid (ab65341, Abcam), triglycerol (ETGA-200, Bioassay Systems, Hayward, CA, USA), cholesterol, and HDL (ab65390, Abcam) were used to measure their levels from the liver tissues saved from the experimental diets
Lee H., Kim E., Shin E.A., Shon J.C., Sun H., Kim J.E., Jung J.W., Lee H., Pinanga Y., Song D.G., Liu K.H, & Lee J.W. (2022). Crosstalk between TM4SF5 and GLUT8 regulates fructose metabolism in hepatic steatosis. Molecular Metabolism, 58, 101451.
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