For preparation of animal diet, AIN-76 semi-purified diet, sodium caseinate (ECCO 2300) the product of Vichi Enterprise. Co., Ltd., New York, USA. DL-methionine and vitamin mix were the products of Fluka, Sigma Aldrich Co., Ltd. (CAT No: 59-51-8). Mineral mix (M.W. = 253.3, 500 g; CAT No: 101384; Lot No: 2200 K) and choline bitartrate were purchased from MP Biomedical LLC, Solon, California, USA. Cellulose (SOLKA-FLOC® 200 FCC) was the product from FS&D (St. Louis, Missouri, USA). Vitamin K1 was the product of DSM Nutritional Products Ltd. (Sisseln, Switzerland). Most of the common ingredients used in the study such as corn flour, sucrose and corn oil were obtained locally.
Mineral mix
Manufactured by MP Biomedicals
Sourced in United States, New Zealand
Mineral mix is a balanced formulation of essential minerals and trace elements designed to supplement the nutritional requirements of laboratory animals. This product provides a convenient and standardized source of these vital nutrients to support animal health and well-being in research settings.
Automatically generated - may contain errors
2 protocols using mineral mix
1
Preparation of AIN-76 Animal Diet
2
High-fat Diet Protocol for Drug Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The chow diet was purchased from Gordon’s Specialty Stockfeeds Pty Ltd, NSW, AU. Chow pallets were blended into a powder and drug added at 750 parts per million (p.p.m.) The diet was reconstituted with water and pellets re-modelled and dried by incubation at 46 °C for 2 hours. The total calorie content or energy density of the normal chow diet was 2.6 kcal/g and for high fat diet was 9 kcal/g. The high fat diet was made as follows: casein 522 g (Cottee, AU), sucrose 460 g (Bundaberg Suger Ltd, QLD), starch 386 g (New Zealand Starch Ltd, NZ), mineral mix 102 g (MP Biochemicals, USA), trace minerals 27.6 g (MP Biochemicals, USA), bran 114 g (Allied Mills Pyt Ltd, AU), methionine 6.8 g (Sigma-Aldrich, USA), gelatine 46 g (J. L Stewart & Son Pyt Ltd, AU), choline bitartate 9.2 g (Sigma-Aldrich, USA), safflower oil 75 ml (Proteco Gold Pyt Ltd, QLD), lard 500 g (Osha Brand, CA) and AIN-93 VX VITAMIN MIX 29.6 g (MP Biochemicals, USA). Drug was added at 1,500 p.p.m.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!