Cells were lysed in a buffer containing 50 mM Tris (pH 7.4), 150 mM NaCl, 1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS, and a protease inhibitor cocktail (Beyotime). The primary antibodies used for Western blotting analysis included rabbit anti-HPV16 E7 (1:200, Biorbyt), rabbit anti-VDAC1 (1:300, Proteintech, USA), and rabbit anti-GAPDH (1:3000, Proteintech, USA). Anti-GAPDH was used as an internal standard for all samples. The signals were detected with Lab image (Beyotime, China).
Rabbit anti hpv16 e7
Manufactured by Biorbyt
Sourced in United States
Rabbit anti-HPV16 E7 is a primary antibody that specifically recognizes the E7 protein of human papillomavirus type 16 (HPV16). This antibody can be used for detection and identification of the HPV16 E7 protein in various research applications.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti vdac1, by Proteintech (2 mentions)
Rabbit anti gapdh, by Proteintech (1 mentions)
Protease inhibitor cocktail, by Beyotime (1 mentions)
Anti vdac3, by Proteintech (1 mentions)
Anti vdac2, by Proteintech (1 mentions)
Image pro plus, by Media Cybernetics (1 mentions)
Cellsens dimension, by Olympus (1 mentions)
3 protocols using rabbit anti hpv16 e7
1
HPV16 E7 Western Blot Analysis
2
Immunohistochemical analysis of VDAC protein expression
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IHC reactions were performed on TMA slides as described elsewhere. The TMA slides were incubated overnight in a humidified chamber at 4°C with primary antibodies: rabbit anti- VDAC1, anti-VDAC2, and anti-VDAC3 (1:300, Proteintech, USA) and rabbit anti-HPV16 E7 (1:200, Biorbyt). Antibody detection was performed with 3,3′-diaminobenzidine (DAB). CellSens Dimension (version 1.8.1, Olympus) were used to photograph the images and the staining intensity was measured with ImagePro Plus (version 6.0, Media Cybernetics).
3
Quantifying HPV16 E7 Expression in Xenografts
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The transplanted tissues (xenografts) in nude mice were removed and fixed with 4% paraformaldehyde. Paraffin-embedded sections (5 μm) were subjected to antigen for 30 min and then treated with 3% hydrogen peroxide for 20 min. Then, the sections were incubated with the antibody overnight at 4 °C (rabbit anti-HPV16 E7 (1:100; Biorbyt), followed by incubation of sections with rabbit secondary antibodies at room temperature. Lastly, the sections were developed with 3,3´-diaminobenzidine (DAB) reagent kit and finally photographed. The intensity was quantified using ImagePro Plus. Using the threshold calculation function of ImageJ, colored areas were selected for area quantification. Then the quantification result of HPV16 E7 was compared with the result of H&E to obtain the percentage of necrotic area.
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