Countess 3 fl cell counter

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Countess 3 FL cell counter is a compact, automated device designed for counting and analyzing cells. It utilizes fluorescence-based detection to provide accurate cell counts and viability measurements. The Countess 3 FL cell counter is a versatile tool for various applications in cell biology research and laboratory settings.

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2 protocols using countess 3 fl cell counter

Cell Counting of BCBL-1 Cells

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BCBL-1 cells (4 × 10⁵/300 μL) were seeded into 48-well plates. Three days after treatment, cells were resuspended by gentle pipetting and then taken out (20 μL) for cell counting. Cell suspensions were mixed with equal volume of trypan blue and counted three times by Countess 3 FL cell counter (Thermo Fisher Scientific Inc, Waltham, MA, USA).

Yeh W.W., Chen Y.Q., Yang W.S., Hong Y.C., Kao S., Liu T.T., Chen T.W., Chang L, & Chang P.C. (2022). SUMO Modification of Histone Demethylase KDM4A in Kaposi’s Sarcoma-Associated Herpesvirus-Induced Primary Effusion Lymphoma. Journal of Virology, 96(16), e00755-22.

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Measuring Cell Proliferation in D2O Media

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Cell numbers at the time of D₂O addition (day 0) and 72 h later (day 3) were determined using a Countess 3FL cell counter (ThermoFisher Scientific, Waltham, MA, USA); proliferation was compared to cells grown in H₂O-based media following our published procedure.^{25 (link)} IC₅₀ values were calculated using an exponential fit:

% proliferation = 100 \times e^{- 0.04 \times (% D_{2} O in medium)}

Jandova J., Galons J.P., Dettman D.L, & Wondrak G.T. (2023). Systemic deuteration of SCID mice using the water-isotopologue deuterium oxide (D2O) inhibits tumor growth in an orthotopic bioluminescent model of human pancreatic ductal adenocarcinoma. Molecular carcinogenesis, 62(5), 598-612.

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