The chemical structure of TZD is shown in Figure 1 . TZD bulk powder (purity: ≥98%) was purchased from LKT Laboratories, Inc (Saint Paul, MN). L-tryptophan methyl ester hydrochloride was purchased from Tokyo Chemical Industry Co, Ltd (Tokyo, Japan) and used as an internal standard (IS). Pooled drug-free serum from a healthy volunteer was purchased from Kohjin Bio Co, Ltd (Saitama, Japan) as the blank. Dipotassium hydrogen phosphate, potassium dihydrogen phosphate, and dimethyl sulfoxide were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Acetonitrile and methanol were of HPLC-grade and purchased from Kanto Chemical Co, Inc (Tokyo, Japan). Ultra-pure water was obtained from an ultra-pure water production device (Arium mini; Sartorius Göttingen, Germany).
Dipotassium hydrogen phosphate
Manufactured by Fujifilm
Sourced in Japan
Dipotassium hydrogen phosphate is a chemical compound used as a buffer solution in laboratory settings. It is a white, crystalline solid that is soluble in water and has a neutral pH. The primary function of dipotassium hydrogen phosphate is to maintain a stable pH in various experimental and analytical procedures.
Automatically generated - may contain errors
Lab products found in correlation
Potassium dihydrogen phosphate, by Fujifilm (7 mentions)
Sodium chloride (nacl), by Fujifilm (3 mentions)
Formic acid, by Fujifilm (3 mentions)
Acetonitrile, by Kanto Chemical (2 mentions)
Methanol, by Kanto Chemical (2 mentions)
Acetonitrile, by Nacalai Tesque (2 mentions)
Sodium carbonate, by Nacalai Tesque (2 mentions)
α glucosidase from saccharomyces cerevisiae, by Merck Group (2 mentions)
Acarbose hydrate, by Tokyo Chemical Industry (2 mentions)
Potassium chloride (kcl), by Fujifilm (2 mentions)
Disodium hydrogenphosphate 12 water, by Fujifilm (2 mentions)
Hydrochloric acid (hcl), by Fujifilm (2 mentions)
Ethanol, by Fujifilm (2 mentions)
Qubit 2.0 fluorometer, by Thermo Fisher Scientific (2 mentions)
Arium mini, by Sartorius (1 mentions)
Dimethyl sulfoxide (dmso), by Fujifilm (1 mentions)
L tryptophan methyl ester hydrochloride, by Tokyo Chemical Industry (1 mentions)
Imidazole, by Merck Group (1 mentions)
Tetramethyl ethylenediamine temed, by Fujifilm (1 mentions)
Trizma base, by Merck Group (1 mentions)
14 protocols using dipotassium hydrogen phosphate
1
Determination of TZD in Serum
2
Purification and Characterization of Thermostable Phosphatase
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
TL was provided by Amano Enzyme Inc. (Nagoya,
Japan). TL was dissolved in 100 mM sodium phosphate buffer, pH 6.0,
and purified from impurities using an Amicon ultrafiltration device
(50 kDa MWCO). Dipotassium hydrogen phosphate, sodium chloride, potassium
dihydrogen phosphate, para-nitrophenyl phosphate
(p-NPP), sodium dodecyl sulfate (SDS), hydrochloric
acid, tetramethylethylenediamine (TEMED), and sodium ampicillin were
purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan).
Tryptone and yeast extract were purchased from Difco (Miami). Trizma
base and imidazole were purchased from Sigma Aldrich (Tokyo, Japan).
Isopropyl-β-D-thiogalactopyranoside (IPTG) was purchased from
Takara Bio Inc. (Shiga, Japan). Acrylamide/bis solution 30% was purchased
from Nacalai Tesque, Inc. (Kyoto, Japan). All reagents were used as
received.
Japan). TL was dissolved in 100 mM sodium phosphate buffer, pH 6.0,
and purified from impurities using an Amicon ultrafiltration device
(50 kDa MWCO). Dipotassium hydrogen phosphate, sodium chloride, potassium
dihydrogen phosphate, para-nitrophenyl phosphate
(p-NPP), sodium dodecyl sulfate (SDS), hydrochloric
acid, tetramethylethylenediamine (TEMED), and sodium ampicillin were
purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan).
Tryptone and yeast extract were purchased from Difco (Miami). Trizma
base and imidazole were purchased from Sigma Aldrich (Tokyo, Japan).
Isopropyl-β-D-thiogalactopyranoside (IPTG) was purchased from
Takara Bio Inc. (Shiga, Japan). Acrylamide/bis solution 30% was purchased
from Nacalai Tesque, Inc. (Kyoto, Japan). All reagents were used as
received.
3
Olanzapine Tablet and Suppository Formulations
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Zyprexa® tablets 2.5 mg (containing 2.5 mg olanzapine) were purchased from Eli Lilly Japan K. K. (Hyogo, Japan). Witepsol H-15, S-5, and E-75 suppository molds (2.25 cm3) were purchased from Maruishi Pharmaceutical Co., Ltd. (Osaka, Japan). olanzapine, perchloric acid (60 %), 10 % ammonia solution, dipotassium hydrogen phosphate, and 1 mol/L potassium hydroxide solution were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Tetramethylammonium perchlorate was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Potassium dihydrogen phosphate and acetonitrile were purchased from Nacalai Tesque, Inc. (Kyoto, Japan).
4
Extraction and Isolation of Bioactive Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For extraction and isolation, n-hexane and methanol were purchased from Kanto Chemicals (Osaka, Japan) and Kieselgel 60 F254 purchased from Merck (Darmstadt, Germany) used for thin-layer chromatography (TLC). Deuteromethanol (CD3OD) (Kanto Chemical, Osaka, Japan) was used for preparing NMR samples. The mobile phase used for HPLC and UPLC were acetonitrile (Kanto Chemical, Osaka, Japan) and formic acid (FUJIFILM Wako Pure Chemical, Japan). L-cysteine methyl ester hydrochloride and N, O-bis (trimethylsilyl) trifluoroacetamide (Tokyo Chemical Industry, Tokyo, Japan) and pyridine (Nacalai Tesque, Japan) were used for acid hydrolysis of GC-MS samples. Methanol purchased from Nacalai Tesque used for polarimeter, UV and quantification analysis. Kaempferol as standard for quantification was purchased from Tokyo Chemical Industry (Japan) For IR analysis, KBr was purchased from FUJIFILM Wako Pure Chemical. For the α-glucosidase inhibitory activity assay, α-glucosidase from Saccharomyces cerevisiae was purchased from Sigma Aldrich (Germany). Acarbose hydrate, as the positive control, was obtained from Tokyo Chemical Industry (Tokyo, Japan). Phosphate buffer was prepared by mixing dipotassium hydrogen phosphate and potassium dihydrogen phosphate purchased from FUJIFILM Wako Pure Chemical. Dimethyl sulfoxide, sodium chloride, and sodium carbonate were purchased from Nacalai Tesque.
5
Quantification of Antioxidant Capacity in Larval Fluids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the quantitative determination of the antioxidant capacity of larval body fluids, a spectrophotometric method based on the molybdenum (Mo) blue reaction was employed as previously described (Prieto et al. 1999 (link)). The assay is based on the reduction of Mo(VI) to Mo(V) by the sample analyte and the subsequent formation of a blue-colored phosphate/Mo(V) complex at acidic pH. Larval bodies of control (w1118/Y) and Gclc46/Y second-instar larvae (48 hr AEL) were severed on parafilm, followed by dropping 20 μl distilled water on 40 dissected larvae. Ten microliters of the body-fluid–water mixture was taken and mixed with 100 μl of reagent solution [1.3% ammonium molybdate (Sigma), 0.02% dipotassium hydrogen phosphate (Wako), and 1.4 M sulfuric acid (Nacalai Tesque)]. The samples were incubated in a thermal block at 95° for 90 min. After the samples cooled to room temperature, their absorbance was measured at 695 nm against a blank. As a series (0–100 mg/ml) of solutions of L(+)-ascorbic acid (Nacalai Tesque) were used as the standard, the antioxidant capacity was expressed in ascorbic acid equivalents. Values of antioxidant capacity were also normalized by the amount of protein in larval body fluids, measured by the Bradford protein assay (Bradford 1976 (link)) with a Qubit protein assay kit and a Qubit 2.0 fluorometer (Thermo Fisher Scientific).
6
Polymer Synthesis and Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Commercially available AAm (Nacalai Tesque,
Kyoto, Japan), SS (Tokyo Chemical Industry Corporation, Ltd., Tokyo,
Japan), and bisAA (Tokyo Chemical Industry Corporation, Ltd., Tokyo,
Japan) were used without further purification. DVBS was supplied from
Tosoh Finechem Corporation, Tokyo, Japan) and used as received. VA-044
and APS (Wako Pure Chemical Industries, Osaka, Japan) as the initiators
were used without purification. For the preparation of a PBS solution,
commercial sodium chloride, potassium chloride, disodium hydrogen
phosphate, and dipotassium hydrogen phosphate (Wako Pure Chemical
Industries, Osaka, Japan) were used as received. 1,2-Dimethoxyethane
(Tokyo Chemical Industry Corporation, Ltd., Tokyo, Japan), ethylene
glycol, sodium hydroxide, PFA, sodium lauryl sulfate, and boric acid
(Nacalai Tesque, Kyoto, Japan), and TritonX-100 (Tokyo Chemical Industry
Corporation, Ltd., Tokyo, Japan) were used as received.
Kyoto, Japan), SS (Tokyo Chemical Industry Corporation, Ltd., Tokyo,
Japan), and bisAA (Tokyo Chemical Industry Corporation, Ltd., Tokyo,
Japan) were used without further purification. DVBS was supplied from
Tosoh Finechem Corporation, Tokyo, Japan) and used as received. VA-044
and APS (Wako Pure Chemical Industries, Osaka, Japan) as the initiators
were used without purification. For the preparation of a PBS solution,
commercial sodium chloride, potassium chloride, disodium hydrogen
phosphate, and dipotassium hydrogen phosphate (Wako Pure Chemical
Industries, Osaka, Japan) were used as received. 1,2-Dimethoxyethane
(Tokyo Chemical Industry Corporation, Ltd., Tokyo, Japan), ethylene
glycol, sodium hydroxide, PFA, sodium lauryl sulfate, and boric acid
(Nacalai Tesque, Kyoto, Japan), and TritonX-100 (Tokyo Chemical Industry
Corporation, Ltd., Tokyo, Japan) were used as received.
7
Quantitative Analysis of Serotonin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serotonin Creatinine Sulfate Monohydrate (serotonin) for the serotonin standard and isotope-labeled Serotonin Creatinine Sulfate Monohydrate (α, α, β, β-d4, 98%, serotonin-d4) for the internal standard (IS) were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan) and Otsuka Pharmaceutical Co., Ltd. (Tokyo, Japan), respectively. Each of these materials was dissolved in a water–methanol mixture (water/methanol=40/60%, v/v) to prepare a 1 mM stock solution, which was then diluted to prepare samples for method evaluation, quality controls, and calibration curves.
Dipotassium hydrogen phosphate and potassium dihydrogen phosphate were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Two aqueous solutions of the same concentration of Dipotassium hydrogen phosphate and potassium dihydrogen phosphate were mixed at a ratio of 6 : 4 to prepare a 10 mM phosphate buffer at pH 6.8. Acetonitrile, formic acid, and 25% ammonia water were purchased from FUJIFILM Wako Pure Chemical Corporation. A 7.5% ammonia solution (water/methanol=30/70%, v/v) was prepared by mixing 25% ammonia water and methanol at a ratio of 3 : 7.
We used spin centrifuge columns for the SPE. The cation exchange column, MonoSpin© CBA, was purchased from GL Science Inc. (Tokyo, Japan).
Dipotassium hydrogen phosphate and potassium dihydrogen phosphate were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Two aqueous solutions of the same concentration of Dipotassium hydrogen phosphate and potassium dihydrogen phosphate were mixed at a ratio of 6 : 4 to prepare a 10 mM phosphate buffer at pH 6.8. Acetonitrile, formic acid, and 25% ammonia water were purchased from FUJIFILM Wako Pure Chemical Corporation. A 7.5% ammonia solution (water/methanol=30/70%, v/v) was prepared by mixing 25% ammonia water and methanol at a ratio of 3 : 7.
We used spin centrifuge columns for the SPE. The cation exchange column, MonoSpin© CBA, was purchased from GL Science Inc. (Tokyo, Japan).
8
Enzymatic Assay of Glucoside Hydrolysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
4MU-αGlc and 4MU were obtained from Sigma-Aldrich Co. LLC (Tokyo, Japan). Acarbose, barium hydroxide, citric acid monohydrate, dipotassium hydrogenphosphate, disodium hydrogenphosphate 12-water, dithiothreitol, magnesium chloride hexahydrate, sodium azide, sodium dodecyl sulfate, sodium hydrate, Triton X-100, and zinc sulfate were obtained from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Glycine and potassium chloride were obtained from Nacalai Tesque, Inc. (Kyoto, Japan). EDTA was obtained from Dojindo Laboratories (Kumamoto, Japan).
9
Cloning and Protein Purification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PrimeSTAR® MAX DNA Polymerase and In-Fusion® HD Cloning Kit were purchased from Takara Bio Inc (Shiga, Japan). LB broth (Miller) was purchased from Merck Millipore Corp (Billerica, MA, USA). Tryptone, extract yeast dried, tris (hydroxymethyl) aminomethane (Tris), acrylamide/bis mixed solution (29:1), and Rapid Stain CBB kit were purchased from Nacalai Tesque, Inc. (Kyoto, Japan). Prestained XL-Ladder was purchased from APRO Science Inc (Tokushima, Japan). Hydrochloric acid, sodium chloride, potassium chloride, disodium hydrogenphosphate 12-water, dipotassium hydrogenphosphate, potassium dihydrogen phosphate, glutathione (reduced form), Fe(III) chloride hexahydrate, and trichloroacetic acid were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). N,N,N',N'-tetramethylethylenediamine, ammonium persulfate, and hydroxylamine hydrochloride were purchased from Kishida Chemical Co.,Ltd (Osaka, Japan). Z-QG was purchased from Peptide Institute, Inc. (Osaka, Japan). L-glutamic acid γ-monohydroxamate was purchased from Sigma-Aldrich Co. LLC (St. Louis, MO). Ultrapure water supplied by Milli-Q® Reference Water Purification System (Merck Millipore Corp., Billerica, MA, USA) was used for buffer preparation.
10
Comprehensive Pesticide Analytical Procedures
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Pesticide analytical grade toluene and acetonitrile, LC-MS grade water, and methanol were obtained from Kanto Chemical (Tokyo, Japan). Diatomaceous earth (Celite® 545), analytical grade ammonium acetate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, and pesticide analytical grade sodium chloride were purchased from FUJIFILM Wako Pure Chemical (Osaka, Japan).
Pesticide standards, except for aramite and etrimfos, were procured from Hayashi Pure Chemical (Osaka, Japan), Kanto Chemical, FUJIFILM Wako Pure Chemical, Dr. Ehrenstorfer (Augsburg, Germany), Riedel-de Haën (Seelze, Germany), and Sigma-Aldrich (St. Louis, MO, USA). Stock standard solutions of each pesticide were prepared in acetonitrile or methanol, depending on their solubility, at a concentration of 1 mg/mL. Standard solutions (100 μg/mL in methanol) of aramite and etrimfos were obtained from AccuStandard (New Haven, CT, USA). A mixed standard solution (1 μg/mL) was prepared by mixing the stock standard solutions and diluting with acetonitrile.
Leucine–enkephalin, used as a reference compound in LC–QTOF-MS analyses, was obtained from Waters (Milford, MA, USA). A 1-μg/mL Leucine–enkephalin standard solution was prepared in methanol/water (1:1, v/v).
Pesticide standards, except for aramite and etrimfos, were procured from Hayashi Pure Chemical (Osaka, Japan), Kanto Chemical, FUJIFILM Wako Pure Chemical, Dr. Ehrenstorfer (Augsburg, Germany), Riedel-de Haën (Seelze, Germany), and Sigma-Aldrich (St. Louis, MO, USA). Stock standard solutions of each pesticide were prepared in acetonitrile or methanol, depending on their solubility, at a concentration of 1 mg/mL. Standard solutions (100 μg/mL in methanol) of aramite and etrimfos were obtained from AccuStandard (New Haven, CT, USA). A mixed standard solution (1 μg/mL) was prepared by mixing the stock standard solutions and diluting with acetonitrile.
Leucine–enkephalin, used as a reference compound in LC–QTOF-MS analyses, was obtained from Waters (Milford, MA, USA). A 1-μg/mL Leucine–enkephalin standard solution was prepared in methanol/water (1:1, v/v).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!