B6.NQ mice express a congenic fragment containing the Aq gene from the B10.Q mice [28 (link)] and were kindly provided by Dr. Rikard Holmdahl, Karolinska Institute, Stockholm, Sweden. Terc-/- mice, which exhibit a premature aging phenotype due to telomere shorting and chromosomal instability [29 (link)] were provided by Dr. Lenhard Rudolph, Fritz-Lipmann-Institute (FLI-Leibniz), Jena, Germany, and were backcrossed onto B6.NQ background. DBA/1 mice were purchased from Janvier labs, France. All mice were bred and housed in the animal facility of the University Hospital Jena. All experiments were conducted following approval by the Thüringer Landesamt für Verbraucherschutz, Bad Langensalza, Germany; registration numbers 02–041/14, 02–079/14, 02–028/15, and 02-031/15. The exact age of every mouse analyzed in this study is shown in the Supplementary Tables .
Dba 1 mice
Manufactured by Janvier Labs
Sourced in France, Germany
The DBA/1 mice are a widely used mouse strain in biomedical research. They are an inbred strain that exhibits specific genetic and physiological characteristics. This strain is commonly used as a model for studying various diseases and research applications.
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12 protocols using dba 1 mice
1
Congenic Mouse Models for Telomere Aging
2
Genetically Modified Mouse Models
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Seven-week-old male C57BL/6 mice were purchased from Charles River (Germany). Seven-week-old male DBA/1 mice were purchased from Janvier (Germany). Mice were co-housed for 1 week prior to the start of experiments. The hTNFtg mice (strain Tg197 on C57BL/6 background) have been previously described.18 (link) Evaluation of arthritis in hTNFtg mice initiated 6 weeks after birth and were performed twice a week. The generation of Jun-floxed and LysM-Cre mice has been previously described.19 (link) The mice were bred and maintained on a 129/C57BL/6 background. The generation of Arg-floxed and Tie2-Cre mice on a B6 background has been previously described.20 (link)
Arg-floxed Ctsk-Cre mice on a B6 background were obtained by independent backcrossing of Ctsk-Cre and Arg-floxed mice with C57BL/6 mice for 12 generations and intercrossing thereafter. Littermate mice were used as controls. Mice were age and sex matched and used at 7–12 weeks of age. All mice were housed in a temperature-controlled and humidity-controlled facility with free access to food and water.
Arg-floxed Ctsk-Cre mice on a B6 background were obtained by independent backcrossing of Ctsk-Cre and Arg-floxed mice with C57BL/6 mice for 12 generations and intercrossing thereafter. Littermate mice were used as controls. Mice were age and sex matched and used at 7–12 weeks of age. All mice were housed in a temperature-controlled and humidity-controlled facility with free access to food and water.
3
Acute Articular Inflammation in Mice
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Five week old male DBA/1 mice were purchased from Janvier Labs (Saint Berthevin Cedex, France) and allowed to acclimatize for at least 1 week. Mice were fed with a special diet without chlorophyll for at least 5 days before the start of the experiment (Day 0) to minimize the nonspecific background fluorescent signal from the intestine. AIA, an acute articular inflammation mouse model, was induced by a single intra-articular injection of 10 to 20 µL of complete Freund’s adjuvant into the right ankle joint of each mouse. One mL of CFA contains 1 mg of Mycobacterium tuberculosis-H37Ra, heat-killed and dried, 0.85 mL paraffin oil, and 0.15 mL mannide monooleate. [26 (link)] Weight variation, clinical severity (CS) of inflammation status, and fluorescence from mice were observed daily. Mice were sacrificed after 72 h of observation due to ethical reasons. All animal experiments followed European and French animal experimentation regulations. The project was approved by the local Ethics Committee (CEEA 34, Université Paris Descartes, 5 September 2017 and registered by the French Ministry of Research (number 20123-2019021516441070).
4
Dnam1 Knockout Mouse Experiments
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DBA/1 mice were purchased from Janvier (Le Genest-St-Isle, France). Dnam1−/− mice have been described elsewhere [25 (link)]. C57/BL6 expressing DNAM-1 (dnam1+/+) were also purchased from Janvier (Le Genest-St-Isle, France). All mice were 6–8 weeks of age at the time of experimentation, were fed standard rodent chow and water ad libitum. To prevent from a cage effect, mice on different background or with different treatments were randomly assigned to each cage. The study was approved by the Cochin institute committee on animal care and its registered number is CEEA34.GC.052.12.
5
Murine Knockout Models for PD-1/PD-L1 Study
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Eight-week-old female DBA/1 mice were purchased from Janvier, France. C57BL/6 WT male mice (n = 9) were purchased from Taconic, USA. Knockout (KO) mice, PD-1 KO (n = 9), and PD-L1 KO (n = 10) were all male C57BL/6 mice (pdcd1tm1.1Shr, CD274tm1Shr kindly provided by Prof. Arlene Sharpe), and in total, 28 mice were used in this study. All mice were kept in Scantainers under controlled conditions (21°C–25°C, 30%–60% humidity, and 12-h light/dark cycle).
6
Collagen-Induced Arthritis in Mice
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Collagen-induced arthritis model: male DBA/1 mice (Janvier, France) were immunized with 100μg of type II collagen (CII) (Sigma) emulsified in Complete Freund’s Adjuvant (CFA) intradermally into the tail. Three weeks post-induction, immunized mice were boosted with 100μg of CII in Incomplete Freund’s Adjuvant (IFA). The mice were monitored and evaluated two to three times per week for arthritic incidences. Scoring was done based on the severity and the number of limbs exhibiting arthritic symptoms and was assigned as follows: 0 = No evidence of erythema and swelling, 1 = Erythema and mild swelling confined to the tarsals or ankle joint, 2 = Erythema and mild swelling extending from the ankle to the tarsals, 3 = Erythema and moderate swelling extending from the ankle to metatarsal joints, 4 = Erythema and severe swelling encompass the ankle, foot and digits, or ankylosis of the limb. The mice were maintained under specific pathogen-free conditions with an air conditioning system and a 12-h light/12-h dark cycle. The mice had free access to food and water during the experiments.
7
DBA/1 Mice Housing and Experimentation
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Six- to eight-week-old male DBA/1 mice were obtained from Janvier Labs (Le Genest-Saint-Isle, France), housed at the University of Grenoble animal core facility PHTA (Plateforme de Haute Technologie Animale, agreement #C 38516 10 006 delivered by Direction Départementale de la Protection des Personnes de l’Isère), in individually ventilated cages (5 mice per cage) and fed ad libitum a standard diet and filtered water. All experiments were approved by the local ethic committee as well as French ministry of Research and Innovation, under the number: 2015061815254659. These animal experiments comply with the ARRIVE guidelines [13 (link)]. Some of the experiments have been performed at the UMR1098 animal facility (agreement #C25-056-7; Besançon, France) under #02831 project number authorization.
8
Murine Model for Immunological Studies
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Wild type (WT) C57Bl6/J mice and DBA-1 mice were purchased from Janvier-Elevage (Le Genest Saint Isle, France), and breeding pairs of the IL-21R-deficient mice on a C57Bl6/J background were provided by Pfizer. Breeding pairs of the IL-6−/− mice, back-crossed eight times with C57Bl6/J, were a kind gift from Dr Manfred Kopf (Basel, Switzerland)[23 (link)]. Animals were used between 10 and 12 weeks of age and were housed in filter-top cages under specific pathogen-free conditions. Microbiome synchronization was promoted by housing purchased WT mice near the genetically modified mice for a period of at least one week, before initiating experiments. A standard diet and water were provided ad libitum. Before being sacrificed by cervical dislocation, mice were anesthetized using 2–3% isoflurane. All animal procedures were approved by the ethics committee of the Radboud University Nijmegen (approval numbers 2014–045, 2010–002, and 2012–207).
9
Standardized Husbandry of C3H/HeNRj and DBA/1 Mice
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Six-week-old, female C3H/HeNRj and DBA/1 mice were purchased (Janvier, Le Genest-Saint-Isle, France) and housed in groups of 4–8 mice in individually ventilated cages under standardized lighting conditions and fed pathogen-free food and water ad libitum. All animal experiments had been approved by The Danish Working Environment Authority and by The Animal Experiments Inspectorate in Denmark under the license number 2016-15-0201-00920, approved on 5 July 2016.
10
Liver Fibrosis Induction in Mice
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DBA-1 mice were purchased from Janvier (Le Genest-St-Isles, France). IL-1Ra knockout (IL-1Ra KO) mice were generated from DBA-1 background and were a kind gift from Prof. Cem Gabay of Geneva University [60 (link),61 (link)]. Eight to ten-week-old male mice were used. Liver fibrosis was induced by 2 to 4 weeks of BDL or 6 weeks of CCl-4 treatment, as described here below. All animal studies were approved by the Animal Ethics Committee of the Geneva Veterinarian Office and Geneva University, Geneva, Switzerland (protocol 1043/3603/2, Oct 1, 2010).
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