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Irdye 800 labeled goat anti rabbit igg

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IRDye®800-labeled goat anti-rabbit IgG is a secondary antibody used in immunoassays and other applications. It is labeled with the near-infrared dye IRDye®800 for detection and quantification purposes.

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Lab products found in correlation

Irdye 680 labeled goat anti mouse igg, by LI COR (8 mentions) Bis tris gel, by Thermo Fisher Scientific (7 mentions) M per buffer, by Thermo Fisher Scientific (6 mentions) Odyssey detection system, by LI COR (5 mentions) Protease phosphatase inhibitor cocktail, by Roche (5 mentions) Odyssey clx system, by LI COR (3 mentions) Erf3 gspt1, by Abcam (2 mentions) Protease phosphatase inhibitor cocktail, by Thermo Fisher Scientific (2 mentions) β actin, by Cell Signaling Technology (2 mentions) Phospho p65 s536, by Cell Signaling Technology (1 mentions) Pa5 28612, by Thermo Fisher Scientific (1 mentions) Sc 271737, by Santa Cruz Biotechnology (1 mentions) Ab8227, by Abcam (1 mentions) Aurka, by Cell Signaling Technology (1 mentions) Intercept blocking buffer, by LI COR (1 mentions) Nitrocellulose membrane, by Bio-Rad (1 mentions) Protease and phosphatase inhibitor cocktail, by Roche (1 mentions)

Close spelling variants of this product

IRDye 800 goat anti-rabbit IgG (17 citations) Goat anti-rabbit IRDye 800 (56 citations) IRDye 800-labeled IgG (2 citations) Anti-rabbit IRDye 800 (35 citations) IRDye goat anti-rabbit (11 citations) Goat anti-rabbit 800 (20 citations) IRDye 800 (256 citations)

8 protocols using irdye 800 labeled goat anti rabbit igg

1

Western Blot Analysis of Apoptosis Regulators

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Cells were lysed in M-PER buffer (Thermo Scientific) containing protease/phosphatase inhibitor cocktail (Roche). After assessing protein concentration by BCA assay (Pierce), equal amounts of protein for each sample were loaded into 4–12% Bis-Tris gels (Invitrogen), transferred to nitrocellulose membranes, and immunoblotted with antibodies against Ikaros, Helios, cleaved Caspase-3, and Actin (Cell Signaling); and eRF3/GSPT1 (Abcam). Membranes were detected on an Odyssey detection system (LI-COR Biosciences) after incubation with IRDye®800-labeled goat anti-rabbit IgG and IRDye®680-labeled goat anti-mouse IgG (LI-COR) secondary antibodies.
Wang E.S., Verano A.L., Nowak R.P., Yuan J.C., Donovan K.A., Eleuteri N.A., Yue H., Ngo K.H., Lizotte P.H., Gokhale P.C., Gray N.S, & Fischer E.S. (2021). Acute pharmacological degradation of Helios destabilizes regulatory T cells. Nature chemical biology, 17(6), 711-717.
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2

Protein Expression Analysis by Western Blot

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Cells were lysed in M-PER buffer (Thermo Scientific) containing protease/phosphatase inhibitor cocktail (Roche). Protein concentration was measured using a BCA assay (Pierce). Equivalent amounts of each sample were loaded on 4–12% Bis-Tris gels (Invitrogen), transferred to nitrocellulose membranes, and immunoblotted with antibodies against CDK4, CDK6, β-catenin, active β-catenin, phospho-S536-p65, total p65, and Actin (Cell Signaling); pS172-NFAT2 (R&D); and NFAT2 (Invitrogen). IRDye®800-labeled goat anti-rabbit IgG and IRDye®680-labeled goat anti-mouse IgG (LI-COR) secondary antibodies were purchased for LI-COR, and membranes were detected on an Odyssey detection system (LI-COR Biosciences).
Deng J., Wang E.S., Jenkins R.W., Li S., Dries R., Yates K., Chhabra S., Huang W., Liu H., Aref A.R., Ivanova E., Paweletz C.P., Bowden M., Zhou C.W., Herter-Sprie G.S., Sorrentino J.A., Bisi J.E., Lizotte P.H., Merlino A.A., Quinn M.M., Bufe L.E., Yang A., Zhang Y., Zhang H., Gao P., Chen T., Cavanaugh M.E., Rode A.J., Haines E., Roberts P.J., Strum J.C., Richards W.G., Lorch J.H., Parangi S., Gunda V., Boland G.M., Bueno R., Palakurthi S., Freeman G.J., Ritz J., Haining W.N., Sharpless N.E., Arthanari H., Shapiro G.I., Barbie D.A., Gray N.S, & Wong K.K. (2017). CDK4/6 Inhibition Augments Anti-Tumor Immunity by Enhancing T Cell Activation. Cancer discovery, 8(2), 216-233.
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3

Western Blot Analysis of Cellular Proteins

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Cells were lysed in RIPA buffer (Pierce) containing protease/phosphatase inhibitor cocktail (Thermo Scientific). Protein concentration was measured using a BCA assay (Pierce). Equivalent amounts of each sample were loaded on 4%–12% Bis-Tris gels (Invitrogen), transferred to nitrocellulose membranes, and immunoblotted with antibodies directed against Npm1 (sc-271737, SantaCruz), Pgk1 (PA5–28612, ThermoFisher), Pdk2 (PA5–28612, ThermoFisher), VINC (4650s, CST), and β-actin (Ab8227, Abcam). IRDye 800-labeled goat anti-rabbit IgG and IRDye 680-labeled goat anti-mouse IgG secondary antibodies were purchased from LI-COR Biosciences, and membranes were subjected to an Odyssey detection system (LI-COR Biosciences).
Li F., Ng W.L., Luster T.A., Hu H., Sviderskiy V.O., Dowling C.M., Hollinshead K.E., Zouitine P., Zhang H., Huang Q., Ranieri M., Wang W., Fang Z., Chen T., Deng J., Zhao K., So H.C., Khodadadi-Jamayran A., Xu M., Karatza A., Pyon V., Li S., Pan Y., Labbe K., Almonte C., Poirier J.T., Miller G., Possemato R., Qi J, & Wong K.K. (2020). Epigenetic CRISPR screens identify Npm1 as a therapeutic vulnerability in non-small cell lung cancer. Cancer research, 80(17), 3556-3567.
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4

Western Blot Analysis of Apoptosis Regulators

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Cells were lysed in M-PER buffer (Thermo Scientific) containing protease/phosphatase inhibitor cocktail (Roche). After assessing protein concentration by BCA assay (Pierce), equal amounts of protein for each sample were loaded into 4–12% Bis-Tris gels (Invitrogen), transferred to nitrocellulose membranes, and immunoblotted with antibodies against Ikaros, Helios, cleaved Caspase-3, and Actin (Cell Signaling); and eRF3/GSPT1 (Abcam). Membranes were detected on an Odyssey detection system (LI-COR Biosciences) after incubation with IRDye®800-labeled goat anti-rabbit IgG and IRDye®680-labeled goat anti-mouse IgG (LI-COR) secondary antibodies.
Wang E.S., Verano A.L., Nowak R.P., Yuan J.C., Donovan K.A., Eleuteri N.A., Yue H., Ngo K.H., Lizotte P.H., Gokhale P.C., Gray N.S, & Fischer E.S. (2021). Acute pharmacological degradation of Helios destabilizes regulatory T cells. Nature chemical biology, 17(6), 711-717.
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5

Immunoblotting Analysis of Chromatin Regulators

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Cells were lysed in RIPA buffer (Pierce) containing protease/phosphatase inhibitor cocktail (Thermo Scientific). Protein concentration was measured using the BCA assay (Pierce). Equivalent amounts of each sample were loaded on 4%–12% Bis-Tris gels (Invitrogen), transferred to nitrocellulose membranes, and immunoblotted with antibodies directed against Cas9 (MA1–202, ThermoFisher), Asf1a (2990s, Cell Signaling Technology), Asf1b (PA5–67639, Invitrogen), γH2AX (9718s, CST) and β-actin (Ab8227, Abcam). IRDye 800-labeled goat anti-rabbit IgG and IRDye 680-labeled goat anti-mouse IgG secondary antibodies were purchased from LI-COR Biosciences, and membranes were detected with an Odyssey detection system (LI-COR Biosciences).
Li F., Huang Q., Luster T.A., Hu H., Zhang H., Ng W.L., Khodadadi-Jamayran A., Wang W., Chen T., Deng J., Ranieri M., Fang Z., Pyon V., Dowling C.M., Bagdatlioglu E., Almonte C., Labbe K., Silver H., Rabin A.R., Jani K., Tsirigos A., Papagiannakopoulos T., Hammerman P.S., Velcheti V., Freeman G.J., Qi J., Miller G, & Wong K.K. (2019). In vivo epigenetic CRISPR screen identifies Asf1a as an immunotherapeutic target in Kras-mutant lung adenocarcinoma. Cancer discovery, 10(2), 270-287.
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6

Sts-1 Western Blot Analysis

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For Sts-1 Western blot analysis, cells were lysed in M-PER buffer (Thermo Scientific) containing protease/phosphatase inhibitor cocktail (Roche). Protein concentration was measured using a BCA assay (Pierce). Equivalent amounts of each sample were loaded on 4–12% Bis-Tris gels (Invitrogen), transferred to nitrocellulose membranes, and immunoblotted with antibodies against Sts-1 (Proteintech #19563–1-AP) and β-Actin (Cell Signaling #3700). IRDye®800-labeled goat anti-rabbit IgG and IRDye®680-labeled goat anti-mouse IgG (LI-COR) secondary antibodies were used and detected on an Odyssey CLxsystem.
Hope J.L., Otero D.C., Bae E.A., Stairiker C.J., Palete A.B., Faso H.A., Lin M., Henriquez M.L., Roy S., Seo H., Lei X., Wang E.S., Chow S., Tinoco R., Daniels G.A., Yip K., Campos A.R., Yin J., Adams P.D., Rao A, & Bradley L.M. (2023). PSGL-1 attenuates early TCR signaling to suppress CD8+ T cell progenitor differentiation and elicit terminal CD8+ T cell exhaustion. Cell reports, 42(5), 112436.
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7

Sts-1 Western Blot Analysis

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For Sts-1 Western blot analysis, cells were lysed in M-PER buffer (Thermo Scientific) containing protease/phosphatase inhibitor cocktail (Roche). Protein concentration was measured using a BCA assay (Pierce). Equivalent amounts of each sample were loaded on 4–12% Bis-Tris gels (Invitrogen), transferred to nitrocellulose membranes, and immunoblotted with antibodies against Sts-1 (Proteintech #19563–1-AP) and β-Actin (Cell Signaling #3700). IRDye®800-labeled goat anti-rabbit IgG and IRDye®680-labeled goat anti-mouse IgG (LI-COR) secondary antibodies were used and detected on an Odyssey CLxsystem.
Hope J.L., Otero D.C., Bae E.A., Stairiker C.J., Palete A.B., Faso H.A., Lin M., Henriquez M.L., Roy S., Seo H., Lei X., Wang E.S., Chow S., Tinoco R., Daniels G.A., Yip K., Campos A.R., Yin J., Adams P.D., Rao A, & Bradley L.M. (2023). PSGL-1 attenuates early TCR signaling to suppress CD8+ T cell progenitor differentiation and elicit terminal CD8+ T cell exhaustion. Cell reports, 42(5), 112436.
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8

Western Blot Analysis of Protein Targets

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Cells were lysed in M-PER buffer (Thermo Scientific) containing protease and phosphatase inhibitor cocktail (Roche). Lysate concentrations were measured and normalized using a BCA assay (Pierce). Equal amounts of lysates were loaded onto 4–12% Bis-Tris gels (Invitrogen), transferred to nitrocellulose membranes (BioRad), and blocked with Intercept blocking buffer (LI-COR). Membranes were then incubated with primary antibodies against ERK5 (Cell Signaling, #3372S), AURKA (Cell Signaling, # 14475S), BRD4 (Fortis Life Sciences, # A301-985A-M), and Actin (Cell Signaling, # 3700S) overnight at 4 °C, followed by incubation with IRDye®800-labeled goat anti-rabbit IgG and IRDye®680-labeled goat anti-mouse IgG (LI-COR) secondary antibodies for detection on an Odyssey CLx System.
You I., Donovan K.A., Krupnick N.M., Boghossian A.S., Rees M.G., Ronan M.M., Roth J.A., Fischer E.S., Wang E.S, & Gray N.S. (2022). Acute pharmacological degradation of ERK5 does not inhibit cellular immune response or proliferation. Cell chemical biology, 29(11), 1630-1638.e7.
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